Genetic Diversity in Gorkhas: an Autosomal STR Study

Genotyping of highly polymorphic autosomal short tandem repeat (STR) markers is a potent tool for elucidating genetic diversity. In the present study, fifteen autosomal STR markers were analyzed in unrelated healthy male Gorkha individuals (n = 98) serving in the Indian Army by using AmpFlSTR Identi...

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Main Authors:	Preet, Kiran, Malhotra, Seema, Shrivastava, Pankaj, Jain, Toshi, Rawat, Shweta, Varte, L. Robert, Singh, Sayar, Singh, Inderjeet, Sarkar, Soma
Format:	Online
Language:	English
Published:	Nature Publishing Group 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5007509/

id	pubmed-5007509
recordtype	oai_dc
spelling	pubmed-50075092016-09-07 Genetic Diversity in Gorkhas: an Autosomal STR Study Preet, Kiran Malhotra, Seema Shrivastava, Pankaj Jain, Toshi Rawat, Shweta Varte, L. Robert Singh, Sayar Singh, Inderjeet Sarkar, Soma Article Genotyping of highly polymorphic autosomal short tandem repeat (STR) markers is a potent tool for elucidating genetic diversity. In the present study, fifteen autosomal STR markers were analyzed in unrelated healthy male Gorkha individuals (n = 98) serving in the Indian Army by using AmpFlSTR Identifiler Plus PCR Amplification Kit. In total, 138 alleles were observed with corresponding allele frequencies ranging from 0.005 to 0.469. The studied loci were in Hardy-Weinberg Equilibrium (HWE). Heterozygosity ranged from 0.602 to 0.867. The most polymorphic locus was Fibrinogen Alpha (FGA) chain which was also the most discriminating locus as expected. Neighbor Joining (NJ) tree and principal component analysis (PCA) plot clustered the Gorkhas with those of Nepal and other Tibeto-Burman population while lowlander Indian population formed separate cluster substantiating the closeness of the Gorkhas with the Tibeto-Burman linguistic phyla. Furthermore, the dataset of STR markers obtained in the study presents a valuable information source of STR DNA profiles from personnel for usage in disaster victim identification in military exigencies and adds to the Indian database of military soldiers and military hospital repository. Nature Publishing Group 2016-09-01 /pmc/articles/PMC5007509/ /pubmed/27580933 http://dx.doi.org/10.1038/srep32494 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Preet, Kiran Malhotra, Seema Shrivastava, Pankaj Jain, Toshi Rawat, Shweta Varte, L. Robert Singh, Sayar Singh, Inderjeet Sarkar, Soma
spellingShingle	Preet, Kiran Malhotra, Seema Shrivastava, Pankaj Jain, Toshi Rawat, Shweta Varte, L. Robert Singh, Sayar Singh, Inderjeet Sarkar, Soma Genetic Diversity in Gorkhas: an Autosomal STR Study
author_facet	Preet, Kiran Malhotra, Seema Shrivastava, Pankaj Jain, Toshi Rawat, Shweta Varte, L. Robert Singh, Sayar Singh, Inderjeet Sarkar, Soma
author_sort	Preet, Kiran
title	Genetic Diversity in Gorkhas: an Autosomal STR Study
title_short	Genetic Diversity in Gorkhas: an Autosomal STR Study
title_full	Genetic Diversity in Gorkhas: an Autosomal STR Study
title_fullStr	Genetic Diversity in Gorkhas: an Autosomal STR Study
title_full_unstemmed	Genetic Diversity in Gorkhas: an Autosomal STR Study
title_sort	genetic diversity in gorkhas: an autosomal str study
description	Genotyping of highly polymorphic autosomal short tandem repeat (STR) markers is a potent tool for elucidating genetic diversity. In the present study, fifteen autosomal STR markers were analyzed in unrelated healthy male Gorkha individuals (n = 98) serving in the Indian Army by using AmpFlSTR Identifiler Plus PCR Amplification Kit. In total, 138 alleles were observed with corresponding allele frequencies ranging from 0.005 to 0.469. The studied loci were in Hardy-Weinberg Equilibrium (HWE). Heterozygosity ranged from 0.602 to 0.867. The most polymorphic locus was Fibrinogen Alpha (FGA) chain which was also the most discriminating locus as expected. Neighbor Joining (NJ) tree and principal component analysis (PCA) plot clustered the Gorkhas with those of Nepal and other Tibeto-Burman population while lowlander Indian population formed separate cluster substantiating the closeness of the Gorkhas with the Tibeto-Burman linguistic phyla. Furthermore, the dataset of STR markers obtained in the study presents a valuable information source of STR DNA profiles from personnel for usage in disaster victim identification in military exigencies and adds to the Indian database of military soldiers and military hospital repository.
publisher	Nature Publishing Group
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5007509/
_version_	1613640810937450496

Genetic Diversity in Gorkhas: an Autosomal STR Study

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