Overexpression of TAZ promotes cell proliferation, migration and epithelial-mesenchymal transition in ovarian cancer

The Hippo pathway is dysregulated in multiple types of human cancer, including ovarian cancer. Nuclear expression of yes-associated protein 1 (YAP1), a downstream transcription coactivator of the Hippo pathway, has been demonstrated to promote tumorigenesis in ovarian cancer and may serve as a poor...

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Main Authors:	Chen, Guangyuan, Xie, Jiabin, Huang, Ping, Yang, Zhihong
Format:	Online
Language:	English
Published:	D.A. Spandidos 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4997911/

id	pubmed-4997911
recordtype	oai_dc
spelling	pubmed-49979112016-09-01 Overexpression of TAZ promotes cell proliferation, migration and epithelial-mesenchymal transition in ovarian cancer Chen, Guangyuan Xie, Jiabin Huang, Ping Yang, Zhihong Articles The Hippo pathway is dysregulated in multiple types of human cancer, including ovarian cancer. Nuclear expression of yes-associated protein 1 (YAP1), a downstream transcription coactivator of the Hippo pathway, has been demonstrated to promote tumorigenesis in ovarian cancer and may serve as a poor prognostic indicator. However, transcriptional coactivator with PDZ binding motif (TAZ), a downstream target of the Hippo pathway and paralog of YAP in mammalian cells, has not been fully investigated in ovarian cancer. The present study aimed to investigate the dysregulation and biological function of TAZ in ovarian cancer. Reverse transcription-quantitative polymerase chain reaction and western blotting revealed that TAZ mRNA and protein levels, respectively, were upregulated in ovarian cancer, and a meta-analysis of ovarian cancer microarray datasets identified that increased expression of TAZ mRNA is correlated with poor prognosis in patients with ovarian cancer. In addition, TAZ-knockdown in ovarian cancer cells demonstrated that TAZ regulates the migration, proliferation and epithelial-mesenchymal transition of ovarian cancer cells. Furthermore, pharmacological disruption of the YAP/TAZ/TEA domain protein complex resulted in a decrease in ovarian cancer cell migration, proliferation and vimentin expression. The results of the present study indicate that the overexpression of TAZ is important in the development and progression of ovarian cancer, and may function as a potential drug target for treatment of this disease entity. D.A. Spandidos 2016-09 2016-07-08 /pmc/articles/PMC4997911/ /pubmed/27588129 http://dx.doi.org/10.3892/ol.2016.4829 Text en Copyright: © Chen et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Chen, Guangyuan Xie, Jiabin Huang, Ping Yang, Zhihong
spellingShingle	Chen, Guangyuan Xie, Jiabin Huang, Ping Yang, Zhihong Overexpression of TAZ promotes cell proliferation, migration and epithelial-mesenchymal transition in ovarian cancer
author_facet	Chen, Guangyuan Xie, Jiabin Huang, Ping Yang, Zhihong
author_sort	Chen, Guangyuan
title	Overexpression of TAZ promotes cell proliferation, migration and epithelial-mesenchymal transition in ovarian cancer
title_short	Overexpression of TAZ promotes cell proliferation, migration and epithelial-mesenchymal transition in ovarian cancer
title_full	Overexpression of TAZ promotes cell proliferation, migration and epithelial-mesenchymal transition in ovarian cancer
title_fullStr	Overexpression of TAZ promotes cell proliferation, migration and epithelial-mesenchymal transition in ovarian cancer
title_full_unstemmed	Overexpression of TAZ promotes cell proliferation, migration and epithelial-mesenchymal transition in ovarian cancer
title_sort	overexpression of taz promotes cell proliferation, migration and epithelial-mesenchymal transition in ovarian cancer
description	The Hippo pathway is dysregulated in multiple types of human cancer, including ovarian cancer. Nuclear expression of yes-associated protein 1 (YAP1), a downstream transcription coactivator of the Hippo pathway, has been demonstrated to promote tumorigenesis in ovarian cancer and may serve as a poor prognostic indicator. However, transcriptional coactivator with PDZ binding motif (TAZ), a downstream target of the Hippo pathway and paralog of YAP in mammalian cells, has not been fully investigated in ovarian cancer. The present study aimed to investigate the dysregulation and biological function of TAZ in ovarian cancer. Reverse transcription-quantitative polymerase chain reaction and western blotting revealed that TAZ mRNA and protein levels, respectively, were upregulated in ovarian cancer, and a meta-analysis of ovarian cancer microarray datasets identified that increased expression of TAZ mRNA is correlated with poor prognosis in patients with ovarian cancer. In addition, TAZ-knockdown in ovarian cancer cells demonstrated that TAZ regulates the migration, proliferation and epithelial-mesenchymal transition of ovarian cancer cells. Furthermore, pharmacological disruption of the YAP/TAZ/TEA domain protein complex resulted in a decrease in ovarian cancer cell migration, proliferation and vimentin expression. The results of the present study indicate that the overexpression of TAZ is important in the development and progression of ovarian cancer, and may function as a potential drug target for treatment of this disease entity.
publisher	D.A. Spandidos
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4997911/
_version_	1613634917580668928

Overexpression of TAZ promotes cell proliferation, migration and epithelial-mesenchymal transition in ovarian cancer

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