Energy metabolism in intestinal epithelial cells during maturation along the crypt-villus axis

Intestinal epithelial cells continuously migrate and mature along crypt-villus axis (CVA), while the changes in energy metabolism during maturation are unclear in neonates. The present study was conducted to test the hypothesis that the energy metabolism in intestinal epithelial cells would be chang...

Full description

Bibliographic Details
Main Authors:	Yang, Huansheng, Wang, Xiaocheng, Xiong, Xia, Yin, Yulong
Format:	Online
Language:	English
Published:	Nature Publishing Group 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4997266/

id	pubmed-4997266
recordtype	oai_dc
spelling	pubmed-49972662016-08-30 Energy metabolism in intestinal epithelial cells during maturation along the crypt-villus axis Yang, Huansheng Wang, Xiaocheng Xiong, Xia Yin, Yulong Article Intestinal epithelial cells continuously migrate and mature along crypt-villus axis (CVA), while the changes in energy metabolism during maturation are unclear in neonates. The present study was conducted to test the hypothesis that the energy metabolism in intestinal epithelial cells would be changed during maturation along CVA in neonates. Eight 21-day-old suckling piglets were used. Intestinal epithelial cells were isolated sequentially along CVA, and proteomics was used to analyze the changes in proteins expression in epithelial cells along CVA. The identified differentially expressed proteins were mainly involved in cellular process, metabolic process, biological regulation, pigmentation, multicellular organizational process and so on. The energy metabolism in intestinal epithelial cells of piglets was increased from the bottom of crypt to the top of villi. Moreover, the expression of proteins related to the metabolism of glucose, most of amino acids, and fatty acids was increased in intestinal epithelial cells during maturation along CVA, while the expression of proteins related to glutamine metabolism was decreased from crypt to villus tip. The expression of proteins involved in citrate cycle was also increased intestinal epithelial cells during maturation along CVA. Moreover, dietary supplementation with different energy sources had different effects on intestinal structure of weaned piglets. Nature Publishing Group 2016-08-25 /pmc/articles/PMC4997266/ /pubmed/27558220 http://dx.doi.org/10.1038/srep31917 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Yang, Huansheng Wang, Xiaocheng Xiong, Xia Yin, Yulong
spellingShingle	Yang, Huansheng Wang, Xiaocheng Xiong, Xia Yin, Yulong Energy metabolism in intestinal epithelial cells during maturation along the crypt-villus axis
author_facet	Yang, Huansheng Wang, Xiaocheng Xiong, Xia Yin, Yulong
author_sort	Yang, Huansheng
title	Energy metabolism in intestinal epithelial cells during maturation along the crypt-villus axis
title_short	Energy metabolism in intestinal epithelial cells during maturation along the crypt-villus axis
title_full	Energy metabolism in intestinal epithelial cells during maturation along the crypt-villus axis
title_fullStr	Energy metabolism in intestinal epithelial cells during maturation along the crypt-villus axis
title_full_unstemmed	Energy metabolism in intestinal epithelial cells during maturation along the crypt-villus axis
title_sort	energy metabolism in intestinal epithelial cells during maturation along the crypt-villus axis
description	Intestinal epithelial cells continuously migrate and mature along crypt-villus axis (CVA), while the changes in energy metabolism during maturation are unclear in neonates. The present study was conducted to test the hypothesis that the energy metabolism in intestinal epithelial cells would be changed during maturation along CVA in neonates. Eight 21-day-old suckling piglets were used. Intestinal epithelial cells were isolated sequentially along CVA, and proteomics was used to analyze the changes in proteins expression in epithelial cells along CVA. The identified differentially expressed proteins were mainly involved in cellular process, metabolic process, biological regulation, pigmentation, multicellular organizational process and so on. The energy metabolism in intestinal epithelial cells of piglets was increased from the bottom of crypt to the top of villi. Moreover, the expression of proteins related to the metabolism of glucose, most of amino acids, and fatty acids was increased in intestinal epithelial cells during maturation along CVA, while the expression of proteins related to glutamine metabolism was decreased from crypt to villus tip. The expression of proteins involved in citrate cycle was also increased intestinal epithelial cells during maturation along CVA. Moreover, dietary supplementation with different energy sources had different effects on intestinal structure of weaned piglets.
publisher	Nature Publishing Group
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4997266/
_version_	1613634443950424064

Energy metabolism in intestinal epithelial cells during maturation along the crypt-villus axis

Similar Items