Properties of Astragalus sp. microsymbionts and their putative role in plant growth promotion
The plant growth-promoting rhizobacteria have developed many different (indirect and direct) mechanisms that have a positive effect on plant growth and development. Strains isolated from Astragaluscicer and Astragalusglycyphyllos root nodules were investigated for their plant growth-promoting proper...
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| Format: | Online |
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Springer Berlin Heidelberg
2016
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4995237/ |
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pubmed-49952372016-09-08 Properties of Astragalus sp. microsymbionts and their putative role in plant growth promotion Wdowiak-Wróbel, Sylwia Małek, Wanda Original Paper The plant growth-promoting rhizobacteria have developed many different (indirect and direct) mechanisms that have a positive effect on plant growth and development. Strains isolated from Astragaluscicer and Astragalusglycyphyllos root nodules were investigated for their plant growth-promoting properties such as production of indole-3-acetic acid (IAA) and siderophores, phosphate solubilization, ACC deaminase activity, and tolerance to heavy metals. IAA production and P-solubilization were frequent features in the analysed strains, while siderophores were not produced by any of them. In this work, we investigated the presence of the acdS genes and ACC deaminase activities in Astragalauscicer and A. glycyphyllos microsymbionts, classified within the genus Mesorhizobium. The results demonstrated that the acdS gene is widespread in the genome of Astragalus sp. microsymbionts; however, none of the tested strains showed ACC deaminase activity. The acdS gene sequence similarity of the analysed strains to each other was in the range from 84 to 99 %. On the phylogram of acdS gene sequences of milkvetch, the symbionts clustered tightly with the genus Mesorhizobium bacteria. Springer Berlin Heidelberg 2016-05-21 2016 /pmc/articles/PMC4995237/ /pubmed/27209414 http://dx.doi.org/10.1007/s00203-016-1243-3 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Wdowiak-Wróbel, Sylwia Małek, Wanda |
| spellingShingle |
Wdowiak-Wróbel, Sylwia Małek, Wanda Properties of Astragalus sp. microsymbionts and their putative role in plant growth promotion |
| author_facet |
Wdowiak-Wróbel, Sylwia Małek, Wanda |
| author_sort |
Wdowiak-Wróbel, Sylwia |
| title |
Properties of Astragalus sp. microsymbionts and their putative role in plant growth promotion |
| title_short |
Properties of Astragalus sp. microsymbionts and their putative role in plant growth promotion |
| title_full |
Properties of Astragalus sp. microsymbionts and their putative role in plant growth promotion |
| title_fullStr |
Properties of Astragalus sp. microsymbionts and their putative role in plant growth promotion |
| title_full_unstemmed |
Properties of Astragalus sp. microsymbionts and their putative role in plant growth promotion |
| title_sort |
properties of astragalus sp. microsymbionts and their putative role in plant growth promotion |
| description |
The plant growth-promoting rhizobacteria have developed many different (indirect and direct) mechanisms that have a positive effect on plant growth and development. Strains isolated from Astragaluscicer and Astragalusglycyphyllos root nodules were investigated for their plant growth-promoting properties such as production of indole-3-acetic acid (IAA) and siderophores, phosphate solubilization, ACC deaminase activity, and tolerance to heavy metals. IAA production and P-solubilization were frequent features in the analysed strains, while siderophores were not produced by any of them. In this work, we investigated the presence of the acdS genes and ACC deaminase activities in Astragalauscicer and A. glycyphyllos microsymbionts, classified within the genus Mesorhizobium. The results demonstrated that the acdS gene is widespread in the genome of Astragalus sp. microsymbionts; however, none of the tested strains showed ACC deaminase activity. The acdS gene sequence similarity of the analysed strains to each other was in the range from 84 to 99 %. On the phylogram of acdS gene sequences of milkvetch, the symbionts clustered tightly with the genus Mesorhizobium bacteria. |
| publisher |
Springer Berlin Heidelberg |
| publishDate |
2016 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4995237/ |
| _version_ |
1613633186003156992 |