Fluorescence-excitation and Emission Spectroscopy on Single FMO Complexes

In green-sulfur bacteria sunlight is absorbed by antenna structures termed chlorosomes, and transferred to the RC via the Fenna-Matthews-Olson (FMO) complex. FMO consists of three monomers arranged in C3 symmetry where each monomer accommodates eight Bacteriochlorophyll a (BChl a) molecules. It was...

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Main Authors:	Löhner, Alexander, Ashraf , Khuram, Cogdell, Richard J., Köhler, Jürgen
Format:	Online
Language:	English
Published:	Nature Publishing Group 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4992959/

id	pubmed-4992959
recordtype	oai_dc
spelling	pubmed-49929592016-08-30 Fluorescence-excitation and Emission Spectroscopy on Single FMO Complexes Löhner, Alexander Ashraf , Khuram Cogdell, Richard J. Köhler, Jürgen Article In green-sulfur bacteria sunlight is absorbed by antenna structures termed chlorosomes, and transferred to the RC via the Fenna-Matthews-Olson (FMO) complex. FMO consists of three monomers arranged in C3 symmetry where each monomer accommodates eight Bacteriochlorophyll a (BChl a) molecules. It was the first pigment-protein complex for which the structure has been determined with high resolution and since then this complex has been the subject of numerous studies both experimentally and theoretically. Here we report about fluorescence-excitation spectroscopy as well as emission spectroscopy from individual FMO complexes at low temperatures. The individual FMO complexes are subjected to very fast spectral fluctuations smearing out any possible different information from the ensemble data that were recorded under the same experimental conditions. In other words, on the time scales that are experimentally accessible by single-molecule techniques, the FMO complex exhibits ergodic behaviour. Nature Publishing Group 2016-08-22 /pmc/articles/PMC4992959/ /pubmed/27545197 http://dx.doi.org/10.1038/srep31875 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Löhner, Alexander Ashraf , Khuram Cogdell, Richard J. Köhler, Jürgen
spellingShingle	Löhner, Alexander Ashraf , Khuram Cogdell, Richard J. Köhler, Jürgen Fluorescence-excitation and Emission Spectroscopy on Single FMO Complexes
author_facet	Löhner, Alexander Ashraf , Khuram Cogdell, Richard J. Köhler, Jürgen
author_sort	Löhner, Alexander
title	Fluorescence-excitation and Emission Spectroscopy on Single FMO Complexes
title_short	Fluorescence-excitation and Emission Spectroscopy on Single FMO Complexes
title_full	Fluorescence-excitation and Emission Spectroscopy on Single FMO Complexes
title_fullStr	Fluorescence-excitation and Emission Spectroscopy on Single FMO Complexes
title_full_unstemmed	Fluorescence-excitation and Emission Spectroscopy on Single FMO Complexes
title_sort	fluorescence-excitation and emission spectroscopy on single fmo complexes
description	In green-sulfur bacteria sunlight is absorbed by antenna structures termed chlorosomes, and transferred to the RC via the Fenna-Matthews-Olson (FMO) complex. FMO consists of three monomers arranged in C3 symmetry where each monomer accommodates eight Bacteriochlorophyll a (BChl a) molecules. It was the first pigment-protein complex for which the structure has been determined with high resolution and since then this complex has been the subject of numerous studies both experimentally and theoretically. Here we report about fluorescence-excitation spectroscopy as well as emission spectroscopy from individual FMO complexes at low temperatures. The individual FMO complexes are subjected to very fast spectral fluctuations smearing out any possible different information from the ensemble data that were recorded under the same experimental conditions. In other words, on the time scales that are experimentally accessible by single-molecule techniques, the FMO complex exhibits ergodic behaviour.
publisher	Nature Publishing Group
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4992959/
_version_	1613631744517341184

Fluorescence-excitation and Emission Spectroscopy on Single FMO Complexes

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