Novel Hydrogenosomes in the Microaerophilic Jakobid Stygiella incarcerata

Mitochondrion-related organelles (MROs) have arisen independently in a wide range of anaerobic protist lineages. Only a few of these organelles and their functions have been investigated in detail, and most of what is known about MROs comes from studies of parasitic organisms such as the parabasalid...

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Main Authors:	Leger, Michelle M., Eme, Laura, Hug, Laura A., Roger, Andrew J.
Format:	Online
Language:	English
Published:	Oxford University Press 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4989108/

id	pubmed-4989108
recordtype	oai_dc
spelling	pubmed-49891082016-08-19 Novel Hydrogenosomes in the Microaerophilic Jakobid Stygiella incarcerata Leger, Michelle M. Eme, Laura Hug, Laura A. Roger, Andrew J. Discoveries Mitochondrion-related organelles (MROs) have arisen independently in a wide range of anaerobic protist lineages. Only a few of these organelles and their functions have been investigated in detail, and most of what is known about MROs comes from studies of parasitic organisms such as the parabasalid Trichomonas vaginalis. Here, we describe the MRO of a free-living anaerobic jakobid excavate, Stygiella incarcerata. We report an RNAseq-based reconstruction of S. incarcerata’s MRO proteome, with an associated biochemical map of the pathways predicted to be present in this organelle. The pyruvate metabolism and oxidative stress response pathways are strikingly similar to those found in the MROs of other anaerobic protists, such as Pygsuia and Trichomonas. This elegant example of convergent evolution is suggestive of an anaerobic biochemical ‘module’ of prokaryotic origins that has been laterally transferred among eukaryotes, enabling them to adapt rapidly to anaerobiosis. We also identified genes corresponding to a variety of mitochondrial processes not found in Trichomonas, including intermembrane space components of the mitochondrial protein import apparatus, and enzymes involved in amino acid metabolism and cardiolipin biosynthesis. In this respect, the MROs of S. incarcerata more closely resemble those of the much more distantly related free-living organisms Pygsuia biforma and Cantina marsupialis, likely reflecting these organisms’ shared lifestyle as free-living anaerobes. Oxford University Press 2016-09 2016-06-08 /pmc/articles/PMC4989108/ /pubmed/27280585 http://dx.doi.org/10.1093/molbev/msw103 Text en © The Author 2016. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Leger, Michelle M. Eme, Laura Hug, Laura A. Roger, Andrew J.
spellingShingle	Leger, Michelle M. Eme, Laura Hug, Laura A. Roger, Andrew J. Novel Hydrogenosomes in the Microaerophilic Jakobid Stygiella incarcerata
author_facet	Leger, Michelle M. Eme, Laura Hug, Laura A. Roger, Andrew J.
author_sort	Leger, Michelle M.
title	Novel Hydrogenosomes in the Microaerophilic Jakobid Stygiella incarcerata
title_short	Novel Hydrogenosomes in the Microaerophilic Jakobid Stygiella incarcerata
title_full	Novel Hydrogenosomes in the Microaerophilic Jakobid Stygiella incarcerata
title_fullStr	Novel Hydrogenosomes in the Microaerophilic Jakobid Stygiella incarcerata
title_full_unstemmed	Novel Hydrogenosomes in the Microaerophilic Jakobid Stygiella incarcerata
title_sort	novel hydrogenosomes in the microaerophilic jakobid stygiella incarcerata
description	Mitochondrion-related organelles (MROs) have arisen independently in a wide range of anaerobic protist lineages. Only a few of these organelles and their functions have been investigated in detail, and most of what is known about MROs comes from studies of parasitic organisms such as the parabasalid Trichomonas vaginalis. Here, we describe the MRO of a free-living anaerobic jakobid excavate, Stygiella incarcerata. We report an RNAseq-based reconstruction of S. incarcerata’s MRO proteome, with an associated biochemical map of the pathways predicted to be present in this organelle. The pyruvate metabolism and oxidative stress response pathways are strikingly similar to those found in the MROs of other anaerobic protists, such as Pygsuia and Trichomonas. This elegant example of convergent evolution is suggestive of an anaerobic biochemical ‘module’ of prokaryotic origins that has been laterally transferred among eukaryotes, enabling them to adapt rapidly to anaerobiosis. We also identified genes corresponding to a variety of mitochondrial processes not found in Trichomonas, including intermembrane space components of the mitochondrial protein import apparatus, and enzymes involved in amino acid metabolism and cardiolipin biosynthesis. In this respect, the MROs of S. incarcerata more closely resemble those of the much more distantly related free-living organisms Pygsuia biforma and Cantina marsupialis, likely reflecting these organisms’ shared lifestyle as free-living anaerobes.
publisher	Oxford University Press
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4989108/
_version_	1613629490674532352

Novel Hydrogenosomes in the Microaerophilic Jakobid Stygiella incarcerata

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