Chemical Ligation and Isotope Labeling to Locate Dynamic Effects during Catalysis by Dihydrofolate Reductase†
Chemical ligation has been used to alter motions in specific regions of dihydrofolate reductase from E. coli and to investigate the effects of localized motional changes on enzyme catalysis. Two isotopic hybrids were prepared; one with the mobile N‐terminal segment containing heavy isotopes (2H, 13C...
| Main Authors: | , , , , , , |
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| Format: | Online |
| Language: | English |
| Published: |
WILEY‐VCH Verlag
2015
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4985705/ |