Prodigiosin inhibits motility and activates bacterial cell death revealing molecular biomarkers of programmed cell death

The antimicrobial activity of prodigiosin from Serratia nematodiphila darsh1, a bacterial pigment was tested against few food borne bacterial pathogens Bacillus cereus, Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli. The mode of action of prodigiosin was studied. Prodigiosin indu...

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Main Authors:	Darshan, N., Manonmani, H. K.
Format:	Online
Language:	English
Published:	Springer Berlin Heidelberg 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4961660/

id	pubmed-4961660
recordtype	oai_dc
spelling	pubmed-49616602016-08-10 Prodigiosin inhibits motility and activates bacterial cell death revealing molecular biomarkers of programmed cell death Darshan, N. Manonmani, H. K. Original Article The antimicrobial activity of prodigiosin from Serratia nematodiphila darsh1, a bacterial pigment was tested against few food borne bacterial pathogens Bacillus cereus, Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli. The mode of action of prodigiosin was studied. Prodigiosin induced bactericidal activity indicating a stereotypical set of biochemical and morphological feature of Programmed cell death (PCD). PCD involves DNA fragmentation, generation of ROS, and expression of a protein with caspase-like substrate specificity in bacterial cells. Prodigiosin was observed to be internalized into bacterial cells and was localized predominantly in the membrane and the nuclear fraction, thus, facilitating intracellular trafficking and then binding of prodigiosin to the bacterial DNA. Corresponding to an increasing concentration of prodigiosin, the level of certain proteases were observed to increase in bacteria studied, thus initiating the onset of PCD. Prodigiosin at a sub-inhibitory concentration inhibits motility of pathogens. Our observations indicated that prodigiosin could be a promising antibacterial agent and could be used in the prevention of bacterial infections. Springer Berlin Heidelberg 2016-07-26 /pmc/articles/PMC4961660/ /pubmed/27460563 http://dx.doi.org/10.1186/s13568-016-0222-z Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Darshan, N. Manonmani, H. K.
spellingShingle	Darshan, N. Manonmani, H. K. Prodigiosin inhibits motility and activates bacterial cell death revealing molecular biomarkers of programmed cell death
author_facet	Darshan, N. Manonmani, H. K.
author_sort	Darshan, N.
title	Prodigiosin inhibits motility and activates bacterial cell death revealing molecular biomarkers of programmed cell death
title_short	Prodigiosin inhibits motility and activates bacterial cell death revealing molecular biomarkers of programmed cell death
title_full	Prodigiosin inhibits motility and activates bacterial cell death revealing molecular biomarkers of programmed cell death
title_fullStr	Prodigiosin inhibits motility and activates bacterial cell death revealing molecular biomarkers of programmed cell death
title_full_unstemmed	Prodigiosin inhibits motility and activates bacterial cell death revealing molecular biomarkers of programmed cell death
title_sort	prodigiosin inhibits motility and activates bacterial cell death revealing molecular biomarkers of programmed cell death
description	The antimicrobial activity of prodigiosin from Serratia nematodiphila darsh1, a bacterial pigment was tested against few food borne bacterial pathogens Bacillus cereus, Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli. The mode of action of prodigiosin was studied. Prodigiosin induced bactericidal activity indicating a stereotypical set of biochemical and morphological feature of Programmed cell death (PCD). PCD involves DNA fragmentation, generation of ROS, and expression of a protein with caspase-like substrate specificity in bacterial cells. Prodigiosin was observed to be internalized into bacterial cells and was localized predominantly in the membrane and the nuclear fraction, thus, facilitating intracellular trafficking and then binding of prodigiosin to the bacterial DNA. Corresponding to an increasing concentration of prodigiosin, the level of certain proteases were observed to increase in bacteria studied, thus initiating the onset of PCD. Prodigiosin at a sub-inhibitory concentration inhibits motility of pathogens. Our observations indicated that prodigiosin could be a promising antibacterial agent and could be used in the prevention of bacterial infections.
publisher	Springer Berlin Heidelberg
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4961660/
_version_	1613615375028584448

Prodigiosin inhibits motility and activates bacterial cell death revealing molecular biomarkers of programmed cell death

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