BK Knockout by TALEN-Mediated Gene Targeting in Osteoblasts: KCNMA1 Determines the Proliferation and Differentiation of Osteoblasts

Large conductance calcium-activated potassium (BK) channels participate in many important physiological functions in excitable tissues such as neurons, cardiac and smooth muscles, whereas the knowledge of BK channels in bone tissues and osteoblasts remains elusive. To investigate the role of BK chan...

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Main Authors:	Hei, Hongya, Gao, Jianjun, Dong, Jibin, Tao, Jie, Tian, Lulu, Pan, Wanma, Wang, Hongyu, Zhang, Xuemei
Format:	Online
Language:	English
Published:	Korean Society for Molecular and Cellular Biology 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4959017/

id	pubmed-4959017
recordtype	oai_dc
spelling	pubmed-49590172016-08-08 BK Knockout by TALEN-Mediated Gene Targeting in Osteoblasts: KCNMA1 Determines the Proliferation and Differentiation of Osteoblasts Hei, Hongya Gao, Jianjun Dong, Jibin Tao, Jie Tian, Lulu Pan, Wanma Wang, Hongyu Zhang, Xuemei Article Large conductance calcium-activated potassium (BK) channels participate in many important physiological functions in excitable tissues such as neurons, cardiac and smooth muscles, whereas the knowledge of BK channels in bone tissues and osteoblasts remains elusive. To investigate the role of BK channels in osteoblasts, we used transcription activator-like effector nuclease (TALEN) to establish a BK knockout cell line on rat ROS17/2.8 osteoblast, and detected the proliferation and mineralization of the BK-knockout cells. Our study found that the BK-knockout cells significantly decreased the ability of proliferation and mineralization as osteoblasts, compared to the wild type cells. The overall expression of osteoblast differentiation marker genes in the BK-knockout cells was significantly lower than that in wild type osteoblast cells. The BK-knockout osteoblast cell line in our study displays a phenotype decrease in osteoblast function which can mimic the pathological state of osteoblast and thus provide a working cell line as a tool for study of osteoblast function and bone related diseases. Korean Society for Molecular and Cellular Biology 2016-07 2016-06-21 /pmc/articles/PMC4959017/ /pubmed/27329042 http://dx.doi.org/10.14348/molcells.2016.0033 Text en © The Korean Society for Molecular and Cellular Biology. All rights reserved. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Hei, Hongya Gao, Jianjun Dong, Jibin Tao, Jie Tian, Lulu Pan, Wanma Wang, Hongyu Zhang, Xuemei
spellingShingle	Hei, Hongya Gao, Jianjun Dong, Jibin Tao, Jie Tian, Lulu Pan, Wanma Wang, Hongyu Zhang, Xuemei BK Knockout by TALEN-Mediated Gene Targeting in Osteoblasts: KCNMA1 Determines the Proliferation and Differentiation of Osteoblasts
author_facet	Hei, Hongya Gao, Jianjun Dong, Jibin Tao, Jie Tian, Lulu Pan, Wanma Wang, Hongyu Zhang, Xuemei
author_sort	Hei, Hongya
title	BK Knockout by TALEN-Mediated Gene Targeting in Osteoblasts: KCNMA1 Determines the Proliferation and Differentiation of Osteoblasts
title_short	BK Knockout by TALEN-Mediated Gene Targeting in Osteoblasts: KCNMA1 Determines the Proliferation and Differentiation of Osteoblasts
title_full	BK Knockout by TALEN-Mediated Gene Targeting in Osteoblasts: KCNMA1 Determines the Proliferation and Differentiation of Osteoblasts
title_fullStr	BK Knockout by TALEN-Mediated Gene Targeting in Osteoblasts: KCNMA1 Determines the Proliferation and Differentiation of Osteoblasts
title_full_unstemmed	BK Knockout by TALEN-Mediated Gene Targeting in Osteoblasts: KCNMA1 Determines the Proliferation and Differentiation of Osteoblasts
title_sort	bk knockout by talen-mediated gene targeting in osteoblasts: kcnma1 determines the proliferation and differentiation of osteoblasts
description	Large conductance calcium-activated potassium (BK) channels participate in many important physiological functions in excitable tissues such as neurons, cardiac and smooth muscles, whereas the knowledge of BK channels in bone tissues and osteoblasts remains elusive. To investigate the role of BK channels in osteoblasts, we used transcription activator-like effector nuclease (TALEN) to establish a BK knockout cell line on rat ROS17/2.8 osteoblast, and detected the proliferation and mineralization of the BK-knockout cells. Our study found that the BK-knockout cells significantly decreased the ability of proliferation and mineralization as osteoblasts, compared to the wild type cells. The overall expression of osteoblast differentiation marker genes in the BK-knockout cells was significantly lower than that in wild type osteoblast cells. The BK-knockout osteoblast cell line in our study displays a phenotype decrease in osteoblast function which can mimic the pathological state of osteoblast and thus provide a working cell line as a tool for study of osteoblast function and bone related diseases.
publisher	Korean Society for Molecular and Cellular Biology
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4959017/
_version_	1613614239290753024

BK Knockout by TALEN-Mediated Gene Targeting in Osteoblasts: KCNMA1 Determines the Proliferation and Differentiation of Osteoblasts

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