Profile of the Spleen Transcriptome in Beef Steers with Variation in Gain and Feed Intake

We have previously identified components of the immune system contributing to feed intake and gain in both the rumen and small intestine of beef steers. In this study, we examined the spleen, a major lymphatic organ near the digestive tract, to determine whether it was also influencing individual fe...

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Main Authors:	Lindholm-Perry, Amanda K., Kern, Rebecca J., Keel, Brittney N., Snelling, Warren M., Kuehn, Larry A., Freetly, Harvey C.
Format:	Online
Language:	English
Published:	Frontiers Media S.A. 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4958634/

id	pubmed-4958634
recordtype	oai_dc
spelling	pubmed-49586342016-08-08 Profile of the Spleen Transcriptome in Beef Steers with Variation in Gain and Feed Intake Lindholm-Perry, Amanda K. Kern, Rebecca J. Keel, Brittney N. Snelling, Warren M. Kuehn, Larry A. Freetly, Harvey C. Genetics We have previously identified components of the immune system contributing to feed intake and gain in both the rumen and small intestine of beef steers. In this study, we examined the spleen, a major lymphatic organ near the digestive tract, to determine whether it was also influencing individual feed efficiency status through immune responses. Animals (n = 16) that were divergent for gain and intake were selected for tissue sampling. The spleen transcriptomes were evaluated by microarray. A total of 1216 genes were identified as differentially expressed. Genes were over-represented in Kyoto encyclopedia of genes and genomes (KEGG) pathways including biological regulation, protein folding, cell communication, immune systems process, response to stress, and RNA metabolic process. Several stress response or heat shock genes including HSPH1, HSPA1A, HSPA4, DNAJB4, DNAJA4, etc., were identified as a stress response functional gene cluster in the low gain-low intake animals. These genes were up-regulated amongst the low gain-low intake animals compared to all other groups. Canonical pathways associated with the differentially expressed genes included the coagulation system, extrinsic prothrombin activation, protein ubiquitination, unfolded protein response, and aldosterone signaling in epithelial cells. An analysis of expressed copy number variable (CNV) genes in the spleen produced some of the same genes and gene families that were differentially expressed. Our data suggests the splenic contribution to some of the underlying variation among gain and intake within this group of animals may be a result of immune function and stress response. In addition, some of the differences in immune response functions may be related to gene copy number. Frontiers Media S.A. 2016-07-25 /pmc/articles/PMC4958634/ /pubmed/27504120 http://dx.doi.org/10.3389/fgene.2016.00127 Text en Copyright © 2016 Lindholm-Perry, Kern, Keel, Snelling, Kuehn and Freetly. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Lindholm-Perry, Amanda K. Kern, Rebecca J. Keel, Brittney N. Snelling, Warren M. Kuehn, Larry A. Freetly, Harvey C.
spellingShingle	Lindholm-Perry, Amanda K. Kern, Rebecca J. Keel, Brittney N. Snelling, Warren M. Kuehn, Larry A. Freetly, Harvey C. Profile of the Spleen Transcriptome in Beef Steers with Variation in Gain and Feed Intake
author_facet	Lindholm-Perry, Amanda K. Kern, Rebecca J. Keel, Brittney N. Snelling, Warren M. Kuehn, Larry A. Freetly, Harvey C.
author_sort	Lindholm-Perry, Amanda K.
title	Profile of the Spleen Transcriptome in Beef Steers with Variation in Gain and Feed Intake
title_short	Profile of the Spleen Transcriptome in Beef Steers with Variation in Gain and Feed Intake
title_full	Profile of the Spleen Transcriptome in Beef Steers with Variation in Gain and Feed Intake
title_fullStr	Profile of the Spleen Transcriptome in Beef Steers with Variation in Gain and Feed Intake
title_full_unstemmed	Profile of the Spleen Transcriptome in Beef Steers with Variation in Gain and Feed Intake
title_sort	profile of the spleen transcriptome in beef steers with variation in gain and feed intake
description	We have previously identified components of the immune system contributing to feed intake and gain in both the rumen and small intestine of beef steers. In this study, we examined the spleen, a major lymphatic organ near the digestive tract, to determine whether it was also influencing individual feed efficiency status through immune responses. Animals (n = 16) that were divergent for gain and intake were selected for tissue sampling. The spleen transcriptomes were evaluated by microarray. A total of 1216 genes were identified as differentially expressed. Genes were over-represented in Kyoto encyclopedia of genes and genomes (KEGG) pathways including biological regulation, protein folding, cell communication, immune systems process, response to stress, and RNA metabolic process. Several stress response or heat shock genes including HSPH1, HSPA1A, HSPA4, DNAJB4, DNAJA4, etc., were identified as a stress response functional gene cluster in the low gain-low intake animals. These genes were up-regulated amongst the low gain-low intake animals compared to all other groups. Canonical pathways associated with the differentially expressed genes included the coagulation system, extrinsic prothrombin activation, protein ubiquitination, unfolded protein response, and aldosterone signaling in epithelial cells. An analysis of expressed copy number variable (CNV) genes in the spleen produced some of the same genes and gene families that were differentially expressed. Our data suggests the splenic contribution to some of the underlying variation among gain and intake within this group of animals may be a result of immune function and stress response. In addition, some of the differences in immune response functions may be related to gene copy number.
publisher	Frontiers Media S.A.
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4958634/
_version_	1613614121554542592

Profile of the Spleen Transcriptome in Beef Steers with Variation in Gain and Feed Intake

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