Molecular Basis for Antigenic Diversity of Genus Betanodavirus

Betanodaviruses are the causative agents of viral nervous necrosis (VNN), a devastating disease for the Mediterranean mariculture. Four different betanodavirus species are recognized, Striped jack-, Redspotted grouper-, Tiger puffer-, and Barfin flounder nervous necrosis virus (SJNNV, RGNNV, TPNNV a...

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Main Authors:	Panzarin, Valentina, Toffan, Anna, Abbadi, Miriam, Buratin, Alessandra, Mancin, Marzia, Braaen, Stine, Olsen, Christel Moræus, Bargelloni, Luca, Rimstad, Espen, Cattoli, Giovanni
Format:	Online
Language:	English
Published:	Public Library of Science 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4954670/

id	pubmed-4954670
recordtype	oai_dc
spelling	pubmed-49546702016-08-08 Molecular Basis for Antigenic Diversity of Genus Betanodavirus Panzarin, Valentina Toffan, Anna Abbadi, Miriam Buratin, Alessandra Mancin, Marzia Braaen, Stine Olsen, Christel Moræus Bargelloni, Luca Rimstad, Espen Cattoli, Giovanni Research Article Betanodaviruses are the causative agents of viral nervous necrosis (VNN), a devastating disease for the Mediterranean mariculture. Four different betanodavirus species are recognized, Striped jack-, Redspotted grouper-, Tiger puffer-, and Barfin flounder nervous necrosis virus (SJNNV, RGNNV, TPNNV and BFNNV), but there is little knowledge on their antigenic properties. In order to describe the serological relationships among different betanodavirus genotypes, serum neutralization assays were performed using rabbit polyclonal antisera against eight fish nodaviruses that cover a wide species-, temporal-, spatial- and genetic range. The results indicate that the SJNNV and RGNNV are antigenically distinct, constituting serotypes A and C, respectively. The TPNNV and BFNNV, the latter representing cold-water betanodaviruses, are antigenically related and cluster within serotype B. The reassortant viruses RGNNV/SJNNV and SJNNV/RGNNV group within serotypes A and C, respectively, indicating that the coat protein encoded by RNA2 acts as major immunoreactivity determinant. Immunostaining of in vitro expressed wild type and chimeric capsid proteins between the RGNNV and the SJNNV species indicated that the C-terminal part of the capsid protein retains the immunoreactive portion. The amino acid (aa) residues determining RGNNV and SJNNV antigenic diversity were mapped to aa residues 217–256 and aa 257–341, respectively. Neutralization of reverse genetics derived chimeric viruses indicated that these areas determine the neutralizing epitopes. The data obtained are crucial for the development of targeted serological tests for the diagnosis of VNN, and informative for development of cross-protective vaccines against various betanodavirus genotypes. Public Library of Science 2016-07-20 /pmc/articles/PMC4954670/ /pubmed/27438093 http://dx.doi.org/10.1371/journal.pone.0158814 Text en © 2016 Panzarin et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Panzarin, Valentina Toffan, Anna Abbadi, Miriam Buratin, Alessandra Mancin, Marzia Braaen, Stine Olsen, Christel Moræus Bargelloni, Luca Rimstad, Espen Cattoli, Giovanni
spellingShingle	Panzarin, Valentina Toffan, Anna Abbadi, Miriam Buratin, Alessandra Mancin, Marzia Braaen, Stine Olsen, Christel Moræus Bargelloni, Luca Rimstad, Espen Cattoli, Giovanni Molecular Basis for Antigenic Diversity of Genus Betanodavirus
author_facet	Panzarin, Valentina Toffan, Anna Abbadi, Miriam Buratin, Alessandra Mancin, Marzia Braaen, Stine Olsen, Christel Moræus Bargelloni, Luca Rimstad, Espen Cattoli, Giovanni
author_sort	Panzarin, Valentina
title	Molecular Basis for Antigenic Diversity of Genus Betanodavirus
title_short	Molecular Basis for Antigenic Diversity of Genus Betanodavirus
title_full	Molecular Basis for Antigenic Diversity of Genus Betanodavirus
title_fullStr	Molecular Basis for Antigenic Diversity of Genus Betanodavirus
title_full_unstemmed	Molecular Basis for Antigenic Diversity of Genus Betanodavirus
title_sort	molecular basis for antigenic diversity of genus betanodavirus
description	Betanodaviruses are the causative agents of viral nervous necrosis (VNN), a devastating disease for the Mediterranean mariculture. Four different betanodavirus species are recognized, Striped jack-, Redspotted grouper-, Tiger puffer-, and Barfin flounder nervous necrosis virus (SJNNV, RGNNV, TPNNV and BFNNV), but there is little knowledge on their antigenic properties. In order to describe the serological relationships among different betanodavirus genotypes, serum neutralization assays were performed using rabbit polyclonal antisera against eight fish nodaviruses that cover a wide species-, temporal-, spatial- and genetic range. The results indicate that the SJNNV and RGNNV are antigenically distinct, constituting serotypes A and C, respectively. The TPNNV and BFNNV, the latter representing cold-water betanodaviruses, are antigenically related and cluster within serotype B. The reassortant viruses RGNNV/SJNNV and SJNNV/RGNNV group within serotypes A and C, respectively, indicating that the coat protein encoded by RNA2 acts as major immunoreactivity determinant. Immunostaining of in vitro expressed wild type and chimeric capsid proteins between the RGNNV and the SJNNV species indicated that the C-terminal part of the capsid protein retains the immunoreactive portion. The amino acid (aa) residues determining RGNNV and SJNNV antigenic diversity were mapped to aa residues 217–256 and aa 257–341, respectively. Neutralization of reverse genetics derived chimeric viruses indicated that these areas determine the neutralizing epitopes. The data obtained are crucial for the development of targeted serological tests for the diagnosis of VNN, and informative for development of cross-protective vaccines against various betanodavirus genotypes.
publisher	Public Library of Science
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4954670/
_version_	1613612412798238720

Molecular Basis for Antigenic Diversity of Genus Betanodavirus

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