Acclimation of metabolism to light in A rabidopsis thaliana: the glucose 6‐phosphate/phosphate translocator GPT2 directs metabolic acclimation

Acclimation of metabolism to light in A rabidopsis thaliana: the glucose 6‐phosphate/phosphate translocator GPT2 directs metabolic acclimation

Mature leaves of plants transferred from low to high light typically increase their photosynthetic capacity. In A rabidopsis thaliana, this dynamic acclimation requires expression of GPT2, a glucose 6‐phosphate/phosphate translocator. Here, we examine the impact of GPT2 on leaf metabolism and photos...

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Main Authors: DYSON, BETH C., ALLWOOD, J. WILLIAM, FEIL, REGINA, XU, YUN, MILLER, MATTHEW, BOWSHER, CAROLINE G., GOODACRE, ROYSTON, LUNN, JOHN E., JOHNSON, GILES N.
Format: Online
Language:English
Published: John Wiley and Sons Inc. 2015
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4949648/
id pubmed-4949648
recordtype oai_dc
spelling pubmed-49496482016-07-28 Acclimation of metabolism to light in A rabidopsis thaliana: the glucose 6‐phosphate/phosphate translocator GPT2 directs metabolic acclimation DYSON, BETH C. ALLWOOD, J. WILLIAM FEIL, REGINA XU, YUN MILLER, MATTHEW BOWSHER, CAROLINE G. GOODACRE, ROYSTON LUNN, JOHN E. JOHNSON, GILES N. Original Articles Mature leaves of plants transferred from low to high light typically increase their photosynthetic capacity. In A rabidopsis thaliana, this dynamic acclimation requires expression of GPT2, a glucose 6‐phosphate/phosphate translocator. Here, we examine the impact of GPT2 on leaf metabolism and photosynthesis. Plants of wild type and of a GPT2 knockout (gpt2.2) grown under low light achieved the same photosynthetic rate despite having different metabolic and transcriptomic strategies. Immediately upon transfer to high light, gpt2.2 plants showed a higher rate of photosynthesis than wild‐type plants (35%); however, over subsequent days, wild‐type plants acclimated photosynthetic capacity, increasing the photosynthesis rate by 100% after 7 d. Wild‐type plants accumulated more starch than gpt2.2 plants throughout acclimation. We suggest that GPT2 activity results in the net import of glucose 6‐phosphate from cytosol to chloroplast, increasing starch synthesis. There was clear acclimation of metabolism, with short‐term changes typically being reversed as plants acclimated. Distinct responses to light were observed in wild‐type and gpt2.2 leaves. Significantly higher levels of sugar phosphates were observed in gpt2.2. We suggest that GPT2 alters the distribution of metabolites between compartments and that this plays an essential role in allowing the cell to interpret environmental signals. John Wiley and Sons Inc. 2015-01-25 2015-07 /pmc/articles/PMC4949648/ /pubmed/25474495 http://dx.doi.org/10.1111/pce.12495 Text en © 2014 The Authors. Plant, Cell & Environment published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author DYSON, BETH C.
ALLWOOD, J. WILLIAM
FEIL, REGINA
XU, YUN
MILLER, MATTHEW
BOWSHER, CAROLINE G.
GOODACRE, ROYSTON
LUNN, JOHN E.
JOHNSON, GILES N.
spellingShingle DYSON, BETH C.
ALLWOOD, J. WILLIAM
FEIL, REGINA
XU, YUN
MILLER, MATTHEW
BOWSHER, CAROLINE G.
GOODACRE, ROYSTON
LUNN, JOHN E.
JOHNSON, GILES N.
Acclimation of metabolism to light in A rabidopsis thaliana: the glucose 6‐phosphate/phosphate translocator GPT2 directs metabolic acclimation
author_facet DYSON, BETH C.
ALLWOOD, J. WILLIAM
FEIL, REGINA
XU, YUN
MILLER, MATTHEW
BOWSHER, CAROLINE G.
GOODACRE, ROYSTON
LUNN, JOHN E.
JOHNSON, GILES N.
author_sort DYSON, BETH C.
title Acclimation of metabolism to light in A rabidopsis thaliana: the glucose 6‐phosphate/phosphate translocator GPT2 directs metabolic acclimation
title_short Acclimation of metabolism to light in A rabidopsis thaliana: the glucose 6‐phosphate/phosphate translocator GPT2 directs metabolic acclimation
title_full Acclimation of metabolism to light in A rabidopsis thaliana: the glucose 6‐phosphate/phosphate translocator GPT2 directs metabolic acclimation
title_fullStr Acclimation of metabolism to light in A rabidopsis thaliana: the glucose 6‐phosphate/phosphate translocator GPT2 directs metabolic acclimation
title_full_unstemmed Acclimation of metabolism to light in A rabidopsis thaliana: the glucose 6‐phosphate/phosphate translocator GPT2 directs metabolic acclimation
title_sort acclimation of metabolism to light in a rabidopsis thaliana: the glucose 6‐phosphate/phosphate translocator gpt2 directs metabolic acclimation
description Mature leaves of plants transferred from low to high light typically increase their photosynthetic capacity. In A rabidopsis thaliana, this dynamic acclimation requires expression of GPT2, a glucose 6‐phosphate/phosphate translocator. Here, we examine the impact of GPT2 on leaf metabolism and photosynthesis. Plants of wild type and of a GPT2 knockout (gpt2.2) grown under low light achieved the same photosynthetic rate despite having different metabolic and transcriptomic strategies. Immediately upon transfer to high light, gpt2.2 plants showed a higher rate of photosynthesis than wild‐type plants (35%); however, over subsequent days, wild‐type plants acclimated photosynthetic capacity, increasing the photosynthesis rate by 100% after 7 d. Wild‐type plants accumulated more starch than gpt2.2 plants throughout acclimation. We suggest that GPT2 activity results in the net import of glucose 6‐phosphate from cytosol to chloroplast, increasing starch synthesis. There was clear acclimation of metabolism, with short‐term changes typically being reversed as plants acclimated. Distinct responses to light were observed in wild‐type and gpt2.2 leaves. Significantly higher levels of sugar phosphates were observed in gpt2.2. We suggest that GPT2 alters the distribution of metabolites between compartments and that this plays an essential role in allowing the cell to interpret environmental signals.
publisher John Wiley and Sons Inc.
publishDate 2015
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4949648/
_version_ 1613611330433974272

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