Non-Triple Helical Form of Type IV Collagen α1 Chain

Non-Triple Helical Form of Type IV Collagen α1 Chain

Type IV collagen with a triple-helical structure composed of three α chains is a major component of basement membrane. Previously, we reported that non-triple helical form of type IV collagen α1 chain (NTHα1(IV)) was isolated from human placenta and the culture media of human cells. In the present s...

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Main Authors: Sugiyama, Hiroaki, Tokunaka, Kazuhiro, Hayashi, Toshihiko, Imamura, Yasutada, Morita, Makoto, Yamato, Masayuki
Format: Online
Language:English
Published: Elsevier 2015
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4945737/
id pubmed-4945737
recordtype oai_dc
spelling pubmed-49457372016-07-20 Non-Triple Helical Form of Type IV Collagen α1 Chain Sugiyama, Hiroaki Tokunaka, Kazuhiro Hayashi, Toshihiko Imamura, Yasutada Morita, Makoto Yamato, Masayuki Article Type IV collagen with a triple-helical structure composed of three α chains is a major component of basement membrane. Previously, we reported that non-triple helical form of type IV collagen α1 chain (NTHα1(IV)) was isolated from human placenta and the culture media of human cells. In the present study, we report on the localization of NTH α1(IV) with a monoclonal antibody #370, exclusively reactive for the nascent chain, in the rabbit tissues. The staining was found on the basement membrane of blood vessels, of endomysium, of nerve, and of kidney but not on epithelial basement membrane. In a rabbit angiogenic model, #370 antibody staining was exclusively observed in neovascular tip region of endothelial cells, where no staining with anti-type IV collagen antibody was seen. Distinct localizations suggest that NTHα1(IV) is produced and stably deposited in endothelial cells and the surroundings under physiological conditions with some physiological roles in relation to the dynamics of vascular system. Elsevier 2015-12-09 /pmc/articles/PMC4945737/ /pubmed/27441234 http://dx.doi.org/10.1016/j.heliyon.2015.e00051 Text en © 2015 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Sugiyama, Hiroaki
Tokunaka, Kazuhiro
Hayashi, Toshihiko
Imamura, Yasutada
Morita, Makoto
Yamato, Masayuki
spellingShingle Sugiyama, Hiroaki
Tokunaka, Kazuhiro
Hayashi, Toshihiko
Imamura, Yasutada
Morita, Makoto
Yamato, Masayuki
Non-Triple Helical Form of Type IV Collagen α1 Chain
author_facet Sugiyama, Hiroaki
Tokunaka, Kazuhiro
Hayashi, Toshihiko
Imamura, Yasutada
Morita, Makoto
Yamato, Masayuki
author_sort Sugiyama, Hiroaki
title Non-Triple Helical Form of Type IV Collagen α1 Chain
title_short Non-Triple Helical Form of Type IV Collagen α1 Chain
title_full Non-Triple Helical Form of Type IV Collagen α1 Chain
title_fullStr Non-Triple Helical Form of Type IV Collagen α1 Chain
title_full_unstemmed Non-Triple Helical Form of Type IV Collagen α1 Chain
title_sort non-triple helical form of type iv collagen α1 chain
description Type IV collagen with a triple-helical structure composed of three α chains is a major component of basement membrane. Previously, we reported that non-triple helical form of type IV collagen α1 chain (NTHα1(IV)) was isolated from human placenta and the culture media of human cells. In the present study, we report on the localization of NTH α1(IV) with a monoclonal antibody #370, exclusively reactive for the nascent chain, in the rabbit tissues. The staining was found on the basement membrane of blood vessels, of endomysium, of nerve, and of kidney but not on epithelial basement membrane. In a rabbit angiogenic model, #370 antibody staining was exclusively observed in neovascular tip region of endothelial cells, where no staining with anti-type IV collagen antibody was seen. Distinct localizations suggest that NTHα1(IV) is produced and stably deposited in endothelial cells and the surroundings under physiological conditions with some physiological roles in relation to the dynamics of vascular system.
publisher Elsevier
publishDate 2015
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4945737/
_version_ 1613609611159404544

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