Morphogenic Protein RodZ Interacts with Sporulation Specific SpoIIE in Bacillus subtilis

The first landmark in sporulation of Bacillus subtilis is the formation of an asymmetric septum followed by selective activation of the transcription factor σF in the resulting smaller cell. How the morphological transformations that occur during sporulation are coupled to cell-specific activation o...

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Main Authors:	Muchová, Katarína, Chromiková, Zuzana, Bradshaw, Niels, Wilkinson, Anthony J., Barák, Imrich
Format:	Online
Language:	English
Published:	Public Library of Science 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4945075/

id	pubmed-4945075
recordtype	oai_dc
spelling	pubmed-49450752016-08-08 Morphogenic Protein RodZ Interacts with Sporulation Specific SpoIIE in Bacillus subtilis Muchová, Katarína Chromiková, Zuzana Bradshaw, Niels Wilkinson, Anthony J. Barák, Imrich Research Article The first landmark in sporulation of Bacillus subtilis is the formation of an asymmetric septum followed by selective activation of the transcription factor σF in the resulting smaller cell. How the morphological transformations that occur during sporulation are coupled to cell-specific activation of transcription is largely unknown. The membrane protein SpoIIE is a constituent of the asymmetric sporulation septum and is a crucial determinant of σF activation. Here we report that the morphogenic protein, RodZ, which is essential for cell shape determination, is additionally required for asymmetric septum formation and sporulation. In cells depleted of RodZ, formation of asymmetric septa is disturbed and σF activation is perturbed. During sporulation, we found that SpoIIE recruits RodZ to the asymmetric septum. Moreover, we detected a direct interaction between SpoIIE and RodZ in vitro and in vivo, indicating that SpoIIE-RodZ may form a complex to coordinate asymmetric septum formation and σF activation. We propose that RodZ could provide a link between the cell shape machinery and the coordinated morphological and developmental transitions required to form a resistant spore. Public Library of Science 2016-07-14 /pmc/articles/PMC4945075/ /pubmed/27415800 http://dx.doi.org/10.1371/journal.pone.0159076 Text en © 2016 Muchová et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Muchová, Katarína Chromiková, Zuzana Bradshaw, Niels Wilkinson, Anthony J. Barák, Imrich
spellingShingle	Muchová, Katarína Chromiková, Zuzana Bradshaw, Niels Wilkinson, Anthony J. Barák, Imrich Morphogenic Protein RodZ Interacts with Sporulation Specific SpoIIE in Bacillus subtilis
author_facet	Muchová, Katarína Chromiková, Zuzana Bradshaw, Niels Wilkinson, Anthony J. Barák, Imrich
author_sort	Muchová, Katarína
title	Morphogenic Protein RodZ Interacts with Sporulation Specific SpoIIE in Bacillus subtilis
title_short	Morphogenic Protein RodZ Interacts with Sporulation Specific SpoIIE in Bacillus subtilis
title_full	Morphogenic Protein RodZ Interacts with Sporulation Specific SpoIIE in Bacillus subtilis
title_fullStr	Morphogenic Protein RodZ Interacts with Sporulation Specific SpoIIE in Bacillus subtilis
title_full_unstemmed	Morphogenic Protein RodZ Interacts with Sporulation Specific SpoIIE in Bacillus subtilis
title_sort	morphogenic protein rodz interacts with sporulation specific spoiie in bacillus subtilis
description	The first landmark in sporulation of Bacillus subtilis is the formation of an asymmetric septum followed by selective activation of the transcription factor σF in the resulting smaller cell. How the morphological transformations that occur during sporulation are coupled to cell-specific activation of transcription is largely unknown. The membrane protein SpoIIE is a constituent of the asymmetric sporulation septum and is a crucial determinant of σF activation. Here we report that the morphogenic protein, RodZ, which is essential for cell shape determination, is additionally required for asymmetric septum formation and sporulation. In cells depleted of RodZ, formation of asymmetric septa is disturbed and σF activation is perturbed. During sporulation, we found that SpoIIE recruits RodZ to the asymmetric septum. Moreover, we detected a direct interaction between SpoIIE and RodZ in vitro and in vivo, indicating that SpoIIE-RodZ may form a complex to coordinate asymmetric septum formation and σF activation. We propose that RodZ could provide a link between the cell shape machinery and the coordinated morphological and developmental transitions required to form a resistant spore.
publisher	Public Library of Science
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4945075/
_version_	1613609465342328832

Morphogenic Protein RodZ Interacts with Sporulation Specific SpoIIE in Bacillus subtilis

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