Rickettsial Disease in the Peruvian Amazon Basin

Using a large, passive, clinic-based surveillance program in Iquitos, Peru, we characterized the prevalence of rickettsial infections among undifferentiated febrile cases and obtained evidence of pathogen transmission in potential domestic reservoir contacts and their ectoparasites. Blood specimens...

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Main Authors:	Kocher, Claudine, Morrison, Amy C., Leguia, Mariana, Loyola, Steev, Castillo, Roger M., Galvez, Hugo A., Astete, Helvio, Flores-Mendoza, Carmen, Ampuero, Julia S., Bausch, Daniel G., Halsey, Eric S., Cespedes, Manuel, Zevallos, Karine, Jiang, Ju, Richards, Allen L.
Format:	Online
Language:	English
Published:	Public Library of Science 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4944934/

id	pubmed-4944934
recordtype	oai_dc
spelling	pubmed-49449342016-08-08 Rickettsial Disease in the Peruvian Amazon Basin Kocher, Claudine Morrison, Amy C. Leguia, Mariana Loyola, Steev Castillo, Roger M. Galvez, Hugo A. Astete, Helvio Flores-Mendoza, Carmen Ampuero, Julia S. Bausch, Daniel G. Halsey, Eric S. Cespedes, Manuel Zevallos, Karine Jiang, Ju Richards, Allen L. Research Article Using a large, passive, clinic-based surveillance program in Iquitos, Peru, we characterized the prevalence of rickettsial infections among undifferentiated febrile cases and obtained evidence of pathogen transmission in potential domestic reservoir contacts and their ectoparasites. Blood specimens from humans and animals were assayed for spotted fever group rickettsiae (SFGR) and typhus group rickettsiae (TGR) by ELISA and/or PCR; ectoparasites were screened by PCR. Logistic regression was used to determine associations between patient history, demographic characteristics of participants and symptoms, clinical findings and outcome of rickettsial infection. Of the 2,054 enrolled participants, almost 2% showed evidence of seroconversion or a 4-fold rise in antibody titers specific for rickettsiae between acute and convalescent blood samples. Of 190 fleas (Ctenocephalides felis) and 60 ticks (Rhipicephalus sanguineus) tested, 185 (97.4%) and 3 (5%), respectively, were positive for Rickettsia spp. Candidatus Rickettsia asemboensis was identified in 100% and 33% of the fleas and ticks tested, respectively. Collectively, our serologic data indicates that human pathogenic SFGR are present in the Peruvian Amazon and pose a significant risk of infection to individuals exposed to wild, domestic and peri-domestic animals and their ectoparasites. Public Library of Science 2016-07-14 /pmc/articles/PMC4944934/ /pubmed/27416029 http://dx.doi.org/10.1371/journal.pntd.0004843 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Kocher, Claudine Morrison, Amy C. Leguia, Mariana Loyola, Steev Castillo, Roger M. Galvez, Hugo A. Astete, Helvio Flores-Mendoza, Carmen Ampuero, Julia S. Bausch, Daniel G. Halsey, Eric S. Cespedes, Manuel Zevallos, Karine Jiang, Ju Richards, Allen L.
spellingShingle	Kocher, Claudine Morrison, Amy C. Leguia, Mariana Loyola, Steev Castillo, Roger M. Galvez, Hugo A. Astete, Helvio Flores-Mendoza, Carmen Ampuero, Julia S. Bausch, Daniel G. Halsey, Eric S. Cespedes, Manuel Zevallos, Karine Jiang, Ju Richards, Allen L. Rickettsial Disease in the Peruvian Amazon Basin
author_facet	Kocher, Claudine Morrison, Amy C. Leguia, Mariana Loyola, Steev Castillo, Roger M. Galvez, Hugo A. Astete, Helvio Flores-Mendoza, Carmen Ampuero, Julia S. Bausch, Daniel G. Halsey, Eric S. Cespedes, Manuel Zevallos, Karine Jiang, Ju Richards, Allen L.
author_sort	Kocher, Claudine
title	Rickettsial Disease in the Peruvian Amazon Basin
title_short	Rickettsial Disease in the Peruvian Amazon Basin
title_full	Rickettsial Disease in the Peruvian Amazon Basin
title_fullStr	Rickettsial Disease in the Peruvian Amazon Basin
title_full_unstemmed	Rickettsial Disease in the Peruvian Amazon Basin
title_sort	rickettsial disease in the peruvian amazon basin
description	Using a large, passive, clinic-based surveillance program in Iquitos, Peru, we characterized the prevalence of rickettsial infections among undifferentiated febrile cases and obtained evidence of pathogen transmission in potential domestic reservoir contacts and their ectoparasites. Blood specimens from humans and animals were assayed for spotted fever group rickettsiae (SFGR) and typhus group rickettsiae (TGR) by ELISA and/or PCR; ectoparasites were screened by PCR. Logistic regression was used to determine associations between patient history, demographic characteristics of participants and symptoms, clinical findings and outcome of rickettsial infection. Of the 2,054 enrolled participants, almost 2% showed evidence of seroconversion or a 4-fold rise in antibody titers specific for rickettsiae between acute and convalescent blood samples. Of 190 fleas (Ctenocephalides felis) and 60 ticks (Rhipicephalus sanguineus) tested, 185 (97.4%) and 3 (5%), respectively, were positive for Rickettsia spp. Candidatus Rickettsia asemboensis was identified in 100% and 33% of the fleas and ticks tested, respectively. Collectively, our serologic data indicates that human pathogenic SFGR are present in the Peruvian Amazon and pose a significant risk of infection to individuals exposed to wild, domestic and peri-domestic animals and their ectoparasites.
publisher	Public Library of Science
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4944934/
_version_	1613609367465099264

Rickettsial Disease in the Peruvian Amazon Basin

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