Decreased expression of Beclin-1 is significantly associated with a poor prognosis in oral tongue squamous cell carcinoma

The autophagy-related gene Beclin-1 is critical in the regulation of tumourigenesis and progression, but its role in oral tongue squamous cell carcinoma (OTSCC) has not yet been reported. This study aimed to investigate Beclin-1 expression and its significance in OTSCC. Beclin-1 expression was asses...

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Main Authors:	Hu, Zedong, Zhong, Zhaoming, Huang, Shaohui, Wen, Haojie, Chen, Xue, Chu, Hongying, Li, Qiuli, Sun, Chuanzheng
Format:	Online
Language:	English
Published:	D.A. Spandidos 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4940089/

id	pubmed-4940089
recordtype	oai_dc
spelling	pubmed-49400892016-07-21 Decreased expression of Beclin-1 is significantly associated with a poor prognosis in oral tongue squamous cell carcinoma Hu, Zedong Zhong, Zhaoming Huang, Shaohui Wen, Haojie Chen, Xue Chu, Hongying Li, Qiuli Sun, Chuanzheng Articles The autophagy-related gene Beclin-1 is critical in the regulation of tumourigenesis and progression, but its role in oral tongue squamous cell carcinoma (OTSCC) has not yet been reported. This study aimed to investigate Beclin-1 expression and its significance in OTSCC. Beclin-1 expression was assessed by reverse transcription-quantitative polymerase chain reaction or western blot analysis in 14 OTSCC tissues and matched adjacent noncancerous tissues as well as in 5 OTSCC cell lines and a normal tongue epithelial cell line. Beclin-1 protein expression was examined by immunohistochemistry in 133 OTSCC specimens, and the correlation between Beclin-1 expression and clinicopathological features was investigated. Furthermore, MTT and colony formation assays were performed to investigate the effect of Beclin-1 on the proliferation and clonogenicity of OTSCC cells. It was demonstrated that Beclin-1 expression was significantly decreased in the majority of the 14 OTSCC tissues and the 5 OTSCC cell lines relative to the matched non-cancerous tissues and the normal tongue epithelial cell line, respectively. Immunohistochemistry analysis revealed that decreased Beclin-1 expression was significantly correlated with poor differentiation, lymph node metastasis, advanced clinical tumour-node-metastasis stage, and a poor prognosis in patients with OTSCC. The in vitro assays indicated that the overexpression of Beclin-1 significantly inhibits the proliferation and clonogenicity of OTSCC cells. These results demonstrate that Beclin-1 acts as a tumour suppressor in the development or progression of OTSCC and that Beclin-1 may represent a novel prognostic marker for patients with OTSCC. D.A. Spandidos 2016-08 2016-06-23 /pmc/articles/PMC4940089/ /pubmed/27356955 http://dx.doi.org/10.3892/mmr.2016.5437 Text en Copyright: © Hu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Hu, Zedong Zhong, Zhaoming Huang, Shaohui Wen, Haojie Chen, Xue Chu, Hongying Li, Qiuli Sun, Chuanzheng
spellingShingle	Hu, Zedong Zhong, Zhaoming Huang, Shaohui Wen, Haojie Chen, Xue Chu, Hongying Li, Qiuli Sun, Chuanzheng Decreased expression of Beclin-1 is significantly associated with a poor prognosis in oral tongue squamous cell carcinoma
author_facet	Hu, Zedong Zhong, Zhaoming Huang, Shaohui Wen, Haojie Chen, Xue Chu, Hongying Li, Qiuli Sun, Chuanzheng
author_sort	Hu, Zedong
title	Decreased expression of Beclin-1 is significantly associated with a poor prognosis in oral tongue squamous cell carcinoma
title_short	Decreased expression of Beclin-1 is significantly associated with a poor prognosis in oral tongue squamous cell carcinoma
title_full	Decreased expression of Beclin-1 is significantly associated with a poor prognosis in oral tongue squamous cell carcinoma
title_fullStr	Decreased expression of Beclin-1 is significantly associated with a poor prognosis in oral tongue squamous cell carcinoma
title_full_unstemmed	Decreased expression of Beclin-1 is significantly associated with a poor prognosis in oral tongue squamous cell carcinoma
title_sort	decreased expression of beclin-1 is significantly associated with a poor prognosis in oral tongue squamous cell carcinoma
description	The autophagy-related gene Beclin-1 is critical in the regulation of tumourigenesis and progression, but its role in oral tongue squamous cell carcinoma (OTSCC) has not yet been reported. This study aimed to investigate Beclin-1 expression and its significance in OTSCC. Beclin-1 expression was assessed by reverse transcription-quantitative polymerase chain reaction or western blot analysis in 14 OTSCC tissues and matched adjacent noncancerous tissues as well as in 5 OTSCC cell lines and a normal tongue epithelial cell line. Beclin-1 protein expression was examined by immunohistochemistry in 133 OTSCC specimens, and the correlation between Beclin-1 expression and clinicopathological features was investigated. Furthermore, MTT and colony formation assays were performed to investigate the effect of Beclin-1 on the proliferation and clonogenicity of OTSCC cells. It was demonstrated that Beclin-1 expression was significantly decreased in the majority of the 14 OTSCC tissues and the 5 OTSCC cell lines relative to the matched non-cancerous tissues and the normal tongue epithelial cell line, respectively. Immunohistochemistry analysis revealed that decreased Beclin-1 expression was significantly correlated with poor differentiation, lymph node metastasis, advanced clinical tumour-node-metastasis stage, and a poor prognosis in patients with OTSCC. The in vitro assays indicated that the overexpression of Beclin-1 significantly inhibits the proliferation and clonogenicity of OTSCC cells. These results demonstrate that Beclin-1 acts as a tumour suppressor in the development or progression of OTSCC and that Beclin-1 may represent a novel prognostic marker for patients with OTSCC.
publisher	D.A. Spandidos
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4940089/
_version_	1613607121502339072

Decreased expression of Beclin-1 is significantly associated with a poor prognosis in oral tongue squamous cell carcinoma

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