The Bromodomain Testis-Specific Gene (Brdt) Characterization and Expression in Gilthead Seabream, Sparus Aurata, and European Seabass, Dicentrarchus Labrax

Multiple genes and transcription factors are involved in regulation and control of the complex process of sex determination and differentiation of fish species. Also more, several hormonal factors and some environmental conditions can also be adequate spawning strategies and stimuli for inducing rep...

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Main Authors:	Úbeda-Manzanaro, M., Ortiz-Delgado, J.B., Sarasquete, C.
Format:	Online
Language:	English
Published:	PAGEPress Publications, Pavia, Italy 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4933829/

id	pubmed-4933829
recordtype	oai_dc
spelling	pubmed-49338292016-07-18 The Bromodomain Testis-Specific Gene (Brdt) Characterization and Expression in Gilthead Seabream, Sparus Aurata, and European Seabass, Dicentrarchus Labrax Úbeda-Manzanaro, M. Ortiz-Delgado, J.B. Sarasquete, C. Original Paper Multiple genes and transcription factors are involved in regulation and control of the complex process of sex determination and differentiation of fish species. Also more, several hormonal factors and some environmental conditions can also be adequate spawning strategies and stimuli for inducing reproduction of fish species. Brdt gene belongs to the bromodomain-extraterminal domain (BET) family of transcriptional coregulators. In mammals, Brdt gene is almost exclusively expressed in testis. Furthermore, Brdt protein is involved in elongating spermatids, and is required for proper spermatogenesis and male fertility. However, from our understanding of fish species, the role of this gene as key, during gametogenesis, still remains unknown. In this study, two Brdt mRNA transcripts were isolated from two teleostean fish species, gilt-head seabream and European seabass. In both species the shorter form lacked a functional C-terminal domain, which may involve a different function as transcriptional regulator. The pattern of Brdt expression showed that the highest levels occurred in the gonads. Significantly lower levels of expression were detected in brain, pituitary and different organ systems (heart, kidney, gills, among other somatic tissues) from both studied species. In situ hybridization approach evidenced that Brdt mRNA expression was restricted to specific cell-types of the germ line, during both oogenesis and spermatogenesis processes. PAGEPress Publications, Pavia, Italy 2016-05-02 /pmc/articles/PMC4933829/ /pubmed/27349318 http://dx.doi.org/10.4081/ejh.2016.2638 Text en ©Copyright M. Úbeda-Manzanaro et al. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Úbeda-Manzanaro, M. Ortiz-Delgado, J.B. Sarasquete, C.
spellingShingle	Úbeda-Manzanaro, M. Ortiz-Delgado, J.B. Sarasquete, C. The Bromodomain Testis-Specific Gene (Brdt) Characterization and Expression in Gilthead Seabream, Sparus Aurata, and European Seabass, Dicentrarchus Labrax
author_facet	Úbeda-Manzanaro, M. Ortiz-Delgado, J.B. Sarasquete, C.
author_sort	Úbeda-Manzanaro, M.
title	The Bromodomain Testis-Specific Gene (Brdt) Characterization and Expression in Gilthead Seabream, Sparus Aurata, and European Seabass, Dicentrarchus Labrax
title_short	The Bromodomain Testis-Specific Gene (Brdt) Characterization and Expression in Gilthead Seabream, Sparus Aurata, and European Seabass, Dicentrarchus Labrax
title_full	The Bromodomain Testis-Specific Gene (Brdt) Characterization and Expression in Gilthead Seabream, Sparus Aurata, and European Seabass, Dicentrarchus Labrax
title_fullStr	The Bromodomain Testis-Specific Gene (Brdt) Characterization and Expression in Gilthead Seabream, Sparus Aurata, and European Seabass, Dicentrarchus Labrax
title_full_unstemmed	The Bromodomain Testis-Specific Gene (Brdt) Characterization and Expression in Gilthead Seabream, Sparus Aurata, and European Seabass, Dicentrarchus Labrax
title_sort	bromodomain testis-specific gene (brdt) characterization and expression in gilthead seabream, sparus aurata, and european seabass, dicentrarchus labrax
description	Multiple genes and transcription factors are involved in regulation and control of the complex process of sex determination and differentiation of fish species. Also more, several hormonal factors and some environmental conditions can also be adequate spawning strategies and stimuli for inducing reproduction of fish species. Brdt gene belongs to the bromodomain-extraterminal domain (BET) family of transcriptional coregulators. In mammals, Brdt gene is almost exclusively expressed in testis. Furthermore, Brdt protein is involved in elongating spermatids, and is required for proper spermatogenesis and male fertility. However, from our understanding of fish species, the role of this gene as key, during gametogenesis, still remains unknown. In this study, two Brdt mRNA transcripts were isolated from two teleostean fish species, gilt-head seabream and European seabass. In both species the shorter form lacked a functional C-terminal domain, which may involve a different function as transcriptional regulator. The pattern of Brdt expression showed that the highest levels occurred in the gonads. Significantly lower levels of expression were detected in brain, pituitary and different organ systems (heart, kidney, gills, among other somatic tissues) from both studied species. In situ hybridization approach evidenced that Brdt mRNA expression was restricted to specific cell-types of the germ line, during both oogenesis and spermatogenesis processes.
publisher	PAGEPress Publications, Pavia, Italy
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4933829/
_version_	1613604620655919104

The Bromodomain Testis-Specific Gene (Brdt) Characterization and Expression in Gilthead Seabream, Sparus Aurata, and European Seabass, Dicentrarchus Labrax

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