Simple isocratic method for simultaneous determination of caffeine and catechins in tea products by HPLC

Tea is a popular beverage almost all over the world. Many studies show that tea consumption is closely associated with positive health impact. Most of the HPLC methods used for the determination of tea constituents include gradient elution systems which involve expensive instrumentation. The objecti...

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Main Authors:	Fernando, Chamira Dilanka, Soysa, Preethi
Format:	Online
Language:	English
Published:	Springer International Publishing 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4932014/

id	pubmed-4932014
recordtype	oai_dc
spelling	pubmed-49320142016-07-16 Simple isocratic method for simultaneous determination of caffeine and catechins in tea products by HPLC Fernando, Chamira Dilanka Soysa, Preethi Research Tea is a popular beverage almost all over the world. Many studies show that tea consumption is closely associated with positive health impact. Most of the HPLC methods used for the determination of tea constituents include gradient elution systems which involve expensive instrumentation. The objective of this study was to develop a simple, rapid precise and low cost HPLC method for the separation and quantification of catechins and caffeine in tea (Camellia sinensis). The method utilizes a phenyl column (2.1 × 150 mm) with a UV-detector (280 nm) where excellent chromatographic separation of tea components i.e. gallic acid (GA), caffeine (Caf), epicatechin (EC) and (−)-epigallocatechin gallate (EGCG) was achieved. The isocratic elution system of acetonitrile, glacial acetic acid and deionized water (8:1:91 v/v/v) at a flow rate of 0.5 mL/min was involved. This method produced excellent accuracy and precision. Within run and between run precision was less than 7.5 %. The equations for calibration curves were y = 0.117 (±0.010)x + 0.173 (±0.024), y = 0.100 (±0.003)x + 0.045 (±0.019), y = 0.016 (±0.001)x + 0.006 (±0.004), y = 0.025 (±0.001)x−0.025 (±0.007) for GA, Caf, EC and EGCG respectively. The method validation parameters prove that the method is efficient, a simple and adequate for the quantitative determination of principal components in tea samples. Springer International Publishing 2016-07-04 /pmc/articles/PMC4932014/ /pubmed/27429880 http://dx.doi.org/10.1186/s40064-016-2672-9 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Fernando, Chamira Dilanka Soysa, Preethi
spellingShingle	Fernando, Chamira Dilanka Soysa, Preethi Simple isocratic method for simultaneous determination of caffeine and catechins in tea products by HPLC
author_facet	Fernando, Chamira Dilanka Soysa, Preethi
author_sort	Fernando, Chamira Dilanka
title	Simple isocratic method for simultaneous determination of caffeine and catechins in tea products by HPLC
title_short	Simple isocratic method for simultaneous determination of caffeine and catechins in tea products by HPLC
title_full	Simple isocratic method for simultaneous determination of caffeine and catechins in tea products by HPLC
title_fullStr	Simple isocratic method for simultaneous determination of caffeine and catechins in tea products by HPLC
title_full_unstemmed	Simple isocratic method for simultaneous determination of caffeine and catechins in tea products by HPLC
title_sort	simple isocratic method for simultaneous determination of caffeine and catechins in tea products by hplc
description	Tea is a popular beverage almost all over the world. Many studies show that tea consumption is closely associated with positive health impact. Most of the HPLC methods used for the determination of tea constituents include gradient elution systems which involve expensive instrumentation. The objective of this study was to develop a simple, rapid precise and low cost HPLC method for the separation and quantification of catechins and caffeine in tea (Camellia sinensis). The method utilizes a phenyl column (2.1 × 150 mm) with a UV-detector (280 nm) where excellent chromatographic separation of tea components i.e. gallic acid (GA), caffeine (Caf), epicatechin (EC) and (−)-epigallocatechin gallate (EGCG) was achieved. The isocratic elution system of acetonitrile, glacial acetic acid and deionized water (8:1:91 v/v/v) at a flow rate of 0.5 mL/min was involved. This method produced excellent accuracy and precision. Within run and between run precision was less than 7.5 %. The equations for calibration curves were y = 0.117 (±0.010)x + 0.173 (±0.024), y = 0.100 (±0.003)x + 0.045 (±0.019), y = 0.016 (±0.001)x + 0.006 (±0.004), y = 0.025 (±0.001)x−0.025 (±0.007) for GA, Caf, EC and EGCG respectively. The method validation parameters prove that the method is efficient, a simple and adequate for the quantitative determination of principal components in tea samples.
publisher	Springer International Publishing
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4932014/
_version_	1613603924893237248

Simple isocratic method for simultaneous determination of caffeine and catechins in tea products by HPLC

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