Temporal Regulation of the Bacillus subtilis Acetylome and Evidence for a Role of MreB Acetylation in Cell Wall Growth

Temporal Regulation of the Bacillus subtilis Acetylome and Evidence for a Role of MreB Acetylation in Cell Wall Growth

The past decade highlighted Nε-lysine acetylation as a prevalent posttranslational modification in bacteria. However, knowledge regarding the physiological importance and temporal regulation of acetylation has remained limited. To uncover potential regulatory roles for acetylation, we analyzed how a...

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Main Authors: Carabetta, Valerie J., Greco, Todd M., Tanner, Andrew W., Cristea, Ileana M., Dubnau, David
Format: Online
Language:English
Published: American Society for Microbiology 2016
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4927096/
id pubmed-4927096
recordtype oai_dc
spelling pubmed-49270962016-06-29 Temporal Regulation of the Bacillus subtilis Acetylome and Evidence for a Role of MreB Acetylation in Cell Wall Growth Carabetta, Valerie J. Greco, Todd M. Tanner, Andrew W. Cristea, Ileana M. Dubnau, David Research Article The past decade highlighted Nε-lysine acetylation as a prevalent posttranslational modification in bacteria. However, knowledge regarding the physiological importance and temporal regulation of acetylation has remained limited. To uncover potential regulatory roles for acetylation, we analyzed how acetylation patterns and abundances change between growth phases in B. subtilis. To demonstrate that the identification of cell growth-dependent modifications can point to critical regulatory acetylation events, we further characterized MreB, the cell shape-determining protein. Our findings led us to propose a role for MreB acetylation in controlling cell width by restricting cell wall growth. American Society for Microbiology 2016-05-31 /pmc/articles/PMC4927096/ /pubmed/27376153 http://dx.doi.org/10.1128/mSystems.00005-16 Text en Copyright © 2016 Carabetta et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (http://creativecommons.org/licenses/by/4.0/) .
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Carabetta, Valerie J.
Greco, Todd M.
Tanner, Andrew W.
Cristea, Ileana M.
Dubnau, David
spellingShingle Carabetta, Valerie J.
Greco, Todd M.
Tanner, Andrew W.
Cristea, Ileana M.
Dubnau, David
Temporal Regulation of the Bacillus subtilis Acetylome and Evidence for a Role of MreB Acetylation in Cell Wall Growth
author_facet Carabetta, Valerie J.
Greco, Todd M.
Tanner, Andrew W.
Cristea, Ileana M.
Dubnau, David
author_sort Carabetta, Valerie J.
title Temporal Regulation of the Bacillus subtilis Acetylome and Evidence for a Role of MreB Acetylation in Cell Wall Growth
title_short Temporal Regulation of the Bacillus subtilis Acetylome and Evidence for a Role of MreB Acetylation in Cell Wall Growth
title_full Temporal Regulation of the Bacillus subtilis Acetylome and Evidence for a Role of MreB Acetylation in Cell Wall Growth
title_fullStr Temporal Regulation of the Bacillus subtilis Acetylome and Evidence for a Role of MreB Acetylation in Cell Wall Growth
title_full_unstemmed Temporal Regulation of the Bacillus subtilis Acetylome and Evidence for a Role of MreB Acetylation in Cell Wall Growth
title_sort temporal regulation of the bacillus subtilis acetylome and evidence for a role of mreb acetylation in cell wall growth
description The past decade highlighted Nε-lysine acetylation as a prevalent posttranslational modification in bacteria. However, knowledge regarding the physiological importance and temporal regulation of acetylation has remained limited. To uncover potential regulatory roles for acetylation, we analyzed how acetylation patterns and abundances change between growth phases in B. subtilis. To demonstrate that the identification of cell growth-dependent modifications can point to critical regulatory acetylation events, we further characterized MreB, the cell shape-determining protein. Our findings led us to propose a role for MreB acetylation in controlling cell width by restricting cell wall growth.
publisher American Society for Microbiology
publishDate 2016
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4927096/
_version_ 1613601753519882240

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