First Description of the Extended Spectrum-Beta-Lactamase Gene blaCTX-M-109 in Salmonella Grumpensis Strains Isolated from Neonatal Nosocomial Infections in Dakar, Senegal

Nosocomial infections are very common in African hospitals, particularly in neonatal units. These infections are most often caused by bacteria such as Escherichia coli, Klebsiella spp and Staphylococcus spp. Salmonella strains are rarely involved in nosocomial infections. Here, we report the first d...

Full description

Bibliographic Details
Main Authors:	Diop, Amadou, Sambe-Ba, Bissoume, Seck, Abdoulaye, Dia, Mouhamadou Lamine, Timbiné, Lassina Gadi, Niang, Aïssatou Ameth, Ndiaye, El Hadji Momar, Sonko, Mouhamadou Abdoulaye, Wane, Abdoul Aziz, Bercion, Raymond, Ndiaye, Ousmane, Cissé, Moussa Fafa, Gassama-Sow, Amy
Format:	Online
Language:	English
Published:	Public Library of Science 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4927072/

id	pubmed-4927072
recordtype	oai_dc
spelling	pubmed-49270722016-07-18 First Description of the Extended Spectrum-Beta-Lactamase Gene blaCTX-M-109 in Salmonella Grumpensis Strains Isolated from Neonatal Nosocomial Infections in Dakar, Senegal Diop, Amadou Sambe-Ba, Bissoume Seck, Abdoulaye Dia, Mouhamadou Lamine Timbiné, Lassina Gadi Niang, Aïssatou Ameth Ndiaye, El Hadji Momar Sonko, Mouhamadou Abdoulaye Wane, Abdoul Aziz Bercion, Raymond Ndiaye, Ousmane Cissé, Moussa Fafa Gassama-Sow, Amy Research Article Nosocomial infections are very common in African hospitals, particularly in neonatal units. These infections are most often caused by bacteria such as Escherichia coli, Klebsiella spp and Staphylococcus spp. Salmonella strains are rarely involved in nosocomial infections. Here, we report the first description of S. Grumpensis in neonatal infections in Senegal. Seventeen Salmonella strains were isolated from hospitalized infants’ stool samples. The following resistance phenotype was described in strains: AMXRTICRCFR FOXRCFXRCTXRCAZRIMPSATMRNARNORRCIPRTMRGMRTERSXTR. All isolates were susceptible to imipenem, 15 out of 17 produced an extended spectrum ß-lactamase (ESBL). blaOXA-1, blaSHV-1, blaTEM-1, blaCTX-M1 genes were detected in strains 8, 13, 5 and 8, respectively. blaCTX-M1 sequencing revealed the presence of blaCTX-M-109. Thirteen of the 17 Salmonella Grumpensis strains were analyzed by PFGE. These 13 isolates belonged to a single pulsotype and were genotypically identical. This is the first report of neonatal S. Grumpensis infections in Senegal, and the first report of blaCTX-M-109 in the genus Salmonella. Public Library of Science 2016-06-29 /pmc/articles/PMC4927072/ /pubmed/27355480 http://dx.doi.org/10.1371/journal.pone.0157683 Text en © 2016 Diop et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Diop, Amadou Sambe-Ba, Bissoume Seck, Abdoulaye Dia, Mouhamadou Lamine Timbiné, Lassina Gadi Niang, Aïssatou Ameth Ndiaye, El Hadji Momar Sonko, Mouhamadou Abdoulaye Wane, Abdoul Aziz Bercion, Raymond Ndiaye, Ousmane Cissé, Moussa Fafa Gassama-Sow, Amy
spellingShingle	Diop, Amadou Sambe-Ba, Bissoume Seck, Abdoulaye Dia, Mouhamadou Lamine Timbiné, Lassina Gadi Niang, Aïssatou Ameth Ndiaye, El Hadji Momar Sonko, Mouhamadou Abdoulaye Wane, Abdoul Aziz Bercion, Raymond Ndiaye, Ousmane Cissé, Moussa Fafa Gassama-Sow, Amy First Description of the Extended Spectrum-Beta-Lactamase Gene blaCTX-M-109 in Salmonella Grumpensis Strains Isolated from Neonatal Nosocomial Infections in Dakar, Senegal
author_facet	Diop, Amadou Sambe-Ba, Bissoume Seck, Abdoulaye Dia, Mouhamadou Lamine Timbiné, Lassina Gadi Niang, Aïssatou Ameth Ndiaye, El Hadji Momar Sonko, Mouhamadou Abdoulaye Wane, Abdoul Aziz Bercion, Raymond Ndiaye, Ousmane Cissé, Moussa Fafa Gassama-Sow, Amy
author_sort	Diop, Amadou
title	First Description of the Extended Spectrum-Beta-Lactamase Gene blaCTX-M-109 in Salmonella Grumpensis Strains Isolated from Neonatal Nosocomial Infections in Dakar, Senegal
title_short	First Description of the Extended Spectrum-Beta-Lactamase Gene blaCTX-M-109 in Salmonella Grumpensis Strains Isolated from Neonatal Nosocomial Infections in Dakar, Senegal
title_full	First Description of the Extended Spectrum-Beta-Lactamase Gene blaCTX-M-109 in Salmonella Grumpensis Strains Isolated from Neonatal Nosocomial Infections in Dakar, Senegal
title_fullStr	First Description of the Extended Spectrum-Beta-Lactamase Gene blaCTX-M-109 in Salmonella Grumpensis Strains Isolated from Neonatal Nosocomial Infections in Dakar, Senegal
title_full_unstemmed	First Description of the Extended Spectrum-Beta-Lactamase Gene blaCTX-M-109 in Salmonella Grumpensis Strains Isolated from Neonatal Nosocomial Infections in Dakar, Senegal
title_sort	first description of the extended spectrum-beta-lactamase gene blactx-m-109 in salmonella grumpensis strains isolated from neonatal nosocomial infections in dakar, senegal
description	Nosocomial infections are very common in African hospitals, particularly in neonatal units. These infections are most often caused by bacteria such as Escherichia coli, Klebsiella spp and Staphylococcus spp. Salmonella strains are rarely involved in nosocomial infections. Here, we report the first description of S. Grumpensis in neonatal infections in Senegal. Seventeen Salmonella strains were isolated from hospitalized infants’ stool samples. The following resistance phenotype was described in strains: AMXRTICRCFR FOXRCFXRCTXRCAZRIMPSATMRNARNORRCIPRTMRGMRTERSXTR. All isolates were susceptible to imipenem, 15 out of 17 produced an extended spectrum ß-lactamase (ESBL). blaOXA-1, blaSHV-1, blaTEM-1, blaCTX-M1 genes were detected in strains 8, 13, 5 and 8, respectively. blaCTX-M1 sequencing revealed the presence of blaCTX-M-109. Thirteen of the 17 Salmonella Grumpensis strains were analyzed by PFGE. These 13 isolates belonged to a single pulsotype and were genotypically identical. This is the first report of neonatal S. Grumpensis infections in Senegal, and the first report of blaCTX-M-109 in the genus Salmonella.
publisher	Public Library of Science
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4927072/
_version_	1613601744869130240

First Description of the Extended Spectrum-Beta-Lactamase Gene blaCTX-M-109 in Salmonella Grumpensis Strains Isolated from Neonatal Nosocomial Infections in Dakar, Senegal

Similar Items