Aquaporin-11 (AQP11) Expression in the Mouse Brain
Aquaporin-11 (AQP11) is an intracellular aquaporin expressed in various tissues, including brain tissues in mammals. While AQP11-deficient mice have developed fatal polycystic kidneys at one month old, the role of AQP11 in the brain was not well appreciated. In this study, we examined the AQP11 expr...
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| Format: | Online |
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MDPI
2016
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4926395/ |
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pubmed-49263952016-07-06 Aquaporin-11 (AQP11) Expression in the Mouse Brain Koike, Shin Tanaka, Yasuko Matsuzaki, Toshiyuki Morishita, Yoshiyuki Ishibashi, Kenichi Article Aquaporin-11 (AQP11) is an intracellular aquaporin expressed in various tissues, including brain tissues in mammals. While AQP11-deficient mice have developed fatal polycystic kidneys at one month old, the role of AQP11 in the brain was not well appreciated. In this study, we examined the AQP11 expression in the mouse brain and the brain phenotype of AQP11-deficient mice. AQP11 messenger ribonucleic acid (mRNA) and protein were expressed in the brain, but much less than in the thymus and kidney. Immunostaining showed that AQP11 was localized at the epithelium of the choroid plexus and at the endothelium of the brain capillary, suggesting that AQP11 may be involved in water transport at the choroid plexus and blood-brain barrier (BBB) in the brain. The expression of AQP4, another brain AQP expressed at the BBB, was decreased by half in AQP11-deficient mice, thereby suggesting the presence of the interaction between AQP11 and AQP4. The brain of AQP11-deficient mice, however, did not show any morphological abnormalities and the function of the BBB was intact. Our findings provide a novel insight into a water transport mechanism mediated by AQPs in the brain, which may lead to a new therapy for brain edema. MDPI 2016-06-01 /pmc/articles/PMC4926395/ /pubmed/27258268 http://dx.doi.org/10.3390/ijms17060861 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Koike, Shin Tanaka, Yasuko Matsuzaki, Toshiyuki Morishita, Yoshiyuki Ishibashi, Kenichi |
| spellingShingle |
Koike, Shin Tanaka, Yasuko Matsuzaki, Toshiyuki Morishita, Yoshiyuki Ishibashi, Kenichi Aquaporin-11 (AQP11) Expression in the Mouse Brain |
| author_facet |
Koike, Shin Tanaka, Yasuko Matsuzaki, Toshiyuki Morishita, Yoshiyuki Ishibashi, Kenichi |
| author_sort |
Koike, Shin |
| title |
Aquaporin-11 (AQP11) Expression in the Mouse Brain |
| title_short |
Aquaporin-11 (AQP11) Expression in the Mouse Brain |
| title_full |
Aquaporin-11 (AQP11) Expression in the Mouse Brain |
| title_fullStr |
Aquaporin-11 (AQP11) Expression in the Mouse Brain |
| title_full_unstemmed |
Aquaporin-11 (AQP11) Expression in the Mouse Brain |
| title_sort |
aquaporin-11 (aqp11) expression in the mouse brain |
| description |
Aquaporin-11 (AQP11) is an intracellular aquaporin expressed in various tissues, including brain tissues in mammals. While AQP11-deficient mice have developed fatal polycystic kidneys at one month old, the role of AQP11 in the brain was not well appreciated. In this study, we examined the AQP11 expression in the mouse brain and the brain phenotype of AQP11-deficient mice. AQP11 messenger ribonucleic acid (mRNA) and protein were expressed in the brain, but much less than in the thymus and kidney. Immunostaining showed that AQP11 was localized at the epithelium of the choroid plexus and at the endothelium of the brain capillary, suggesting that AQP11 may be involved in water transport at the choroid plexus and blood-brain barrier (BBB) in the brain. The expression of AQP4, another brain AQP expressed at the BBB, was decreased by half in AQP11-deficient mice, thereby suggesting the presence of the interaction between AQP11 and AQP4. The brain of AQP11-deficient mice, however, did not show any morphological abnormalities and the function of the BBB was intact. Our findings provide a novel insight into a water transport mechanism mediated by AQPs in the brain, which may lead to a new therapy for brain edema. |
| publisher |
MDPI |
| publishDate |
2016 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4926395/ |
| _version_ |
1613601444334665728 |