Lactobacillus crispatus inhibits the infectivity of Chlamydia trachomatis elementary bodies, in vitro study

Lactobacillus species dominate the vaginal microbiota of healthy reproductive-age women and protect the genitourinary tract from the attack of several infectious agents. Chlamydia trachomatis, a leading cause of sexually transmitted disease worldwide, can induce severe sequelae, i.e. pelvic inflamma...

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Main Authors:	Nardini, Paola, Ñahui Palomino, Rogers Alberto, Parolin, Carola, Laghi, Luca, Foschi, Claudio, Cevenini, Roberto, Vitali, Beatrice, Marangoni, Antonella
Format:	Online
Language:	English
Published:	Nature Publishing Group 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4926251/

id	pubmed-4926251
recordtype	oai_dc
spelling	pubmed-49262512016-07-01 Lactobacillus crispatus inhibits the infectivity of Chlamydia trachomatis elementary bodies, in vitro study Nardini, Paola Ñahui Palomino, Rogers Alberto Parolin, Carola Laghi, Luca Foschi, Claudio Cevenini, Roberto Vitali, Beatrice Marangoni, Antonella Article Lactobacillus species dominate the vaginal microbiota of healthy reproductive-age women and protect the genitourinary tract from the attack of several infectious agents. Chlamydia trachomatis, a leading cause of sexually transmitted disease worldwide, can induce severe sequelae, i.e. pelvic inflammatory disease, infertility and ectopic pregnancy. In the present study we investigated the interference of Lactobacillus crispatus, L. gasseri and L. vaginalis, known to be dominant species in the vaginal microbiome, with the infection process of C. trachomatis. Lactobacilli exerted a strong inhibitory effect on Chlamydia infectivity mainly through the action of secreted metabolites in a concentration/pH dependent mode. Short contact times were the most effective in the inhibition, suggesting a protective role of lactobacilli in the early steps of Chlamydia infection. The best anti-Chlamydia profile was shown by L. crispatus species. In order to delineate metabolic profiles related to anti-Chlamydia activity, Lactobacillus supernatants were analysed by 1H-NMR. Production of lactate and acidification of the vaginal environment seemed to be crucial for the activity, in addition to the consumption of the carbonate source represented by glucose. The main conclusion of this study is that high concentrations of L. crispatus inhibit infectivity of C. trachomatis in vitro. Nature Publishing Group 2016-06-29 /pmc/articles/PMC4926251/ /pubmed/27354249 http://dx.doi.org/10.1038/srep29024 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Nardini, Paola Ñahui Palomino, Rogers Alberto Parolin, Carola Laghi, Luca Foschi, Claudio Cevenini, Roberto Vitali, Beatrice Marangoni, Antonella
spellingShingle	Nardini, Paola Ñahui Palomino, Rogers Alberto Parolin, Carola Laghi, Luca Foschi, Claudio Cevenini, Roberto Vitali, Beatrice Marangoni, Antonella Lactobacillus crispatus inhibits the infectivity of Chlamydia trachomatis elementary bodies, in vitro study
author_facet	Nardini, Paola Ñahui Palomino, Rogers Alberto Parolin, Carola Laghi, Luca Foschi, Claudio Cevenini, Roberto Vitali, Beatrice Marangoni, Antonella
author_sort	Nardini, Paola
title	Lactobacillus crispatus inhibits the infectivity of Chlamydia trachomatis elementary bodies, in vitro study
title_short	Lactobacillus crispatus inhibits the infectivity of Chlamydia trachomatis elementary bodies, in vitro study
title_full	Lactobacillus crispatus inhibits the infectivity of Chlamydia trachomatis elementary bodies, in vitro study
title_fullStr	Lactobacillus crispatus inhibits the infectivity of Chlamydia trachomatis elementary bodies, in vitro study
title_full_unstemmed	Lactobacillus crispatus inhibits the infectivity of Chlamydia trachomatis elementary bodies, in vitro study
title_sort	lactobacillus crispatus inhibits the infectivity of chlamydia trachomatis elementary bodies, in vitro study
description	Lactobacillus species dominate the vaginal microbiota of healthy reproductive-age women and protect the genitourinary tract from the attack of several infectious agents. Chlamydia trachomatis, a leading cause of sexually transmitted disease worldwide, can induce severe sequelae, i.e. pelvic inflammatory disease, infertility and ectopic pregnancy. In the present study we investigated the interference of Lactobacillus crispatus, L. gasseri and L. vaginalis, known to be dominant species in the vaginal microbiome, with the infection process of C. trachomatis. Lactobacilli exerted a strong inhibitory effect on Chlamydia infectivity mainly through the action of secreted metabolites in a concentration/pH dependent mode. Short contact times were the most effective in the inhibition, suggesting a protective role of lactobacilli in the early steps of Chlamydia infection. The best anti-Chlamydia profile was shown by L. crispatus species. In order to delineate metabolic profiles related to anti-Chlamydia activity, Lactobacillus supernatants were analysed by 1H-NMR. Production of lactate and acidification of the vaginal environment seemed to be crucial for the activity, in addition to the consumption of the carbonate source represented by glucose. The main conclusion of this study is that high concentrations of L. crispatus inhibit infectivity of C. trachomatis in vitro.
publisher	Nature Publishing Group
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4926251/
_version_	1613601360334290944

Lactobacillus crispatus inhibits the infectivity of Chlamydia trachomatis elementary bodies, in vitro study

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