BC1 RNA motifs required for dendritic transport in vivo

BC1 RNA is a small brain specific non-protein coding RNA. It is transported from the cell body into dendrites where it is involved in the fine-tuning translational control. Due to its compactness and established secondary structure, BC1 RNA is an ideal model for investigating the motifs necessary fo...

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Main Authors:	Robeck, Thomas, Skryabin, Boris V., Rozhdestvensky, Timofey S., Skryabin, Anastasiya B., Brosius, Jürgen
Format:	Online
Language:	English
Published:	Nature Publishing Group 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4923876/

id	pubmed-4923876
recordtype	oai_dc
spelling	pubmed-49238762016-06-28 BC1 RNA motifs required for dendritic transport in vivo Robeck, Thomas Skryabin, Boris V. Rozhdestvensky, Timofey S. Skryabin, Anastasiya B. Brosius, Jürgen Article BC1 RNA is a small brain specific non-protein coding RNA. It is transported from the cell body into dendrites where it is involved in the fine-tuning translational control. Due to its compactness and established secondary structure, BC1 RNA is an ideal model for investigating the motifs necessary for dendritic localization. Previously, microinjection of in vitro transcribed BC1 RNA mutants into the soma of cultured primary neurons suggested the importance of RNA motifs for dendritic targeting. These ex vivo experiments identified a single bulged nucleotide (U22) and a putative K-turn (GA motif) structure required for dendritic localization or distal transport, respectively. We generated six transgenic mouse lines (three founders each) containing neuronally expressing BC1 RNA variants on a BC1 RNA knockout mouse background. In contrast to ex vivo data, we did not find indications of reduction or abolition of dendritic BC1 RNA localization in the mutants devoid of the GA motif or the bulged nucleotide. We confirmed the ex vivo data, which showed that the triloop terminal sequence had no consequence on dendritic transport. Interestingly, changing the triloop supporting structure completely abolished dendritic localization of BC1 RNA. We propose a novel RNA motif important for dendritic transport in vivo. Nature Publishing Group 2016-06-28 /pmc/articles/PMC4923876/ /pubmed/27350115 http://dx.doi.org/10.1038/srep28300 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Robeck, Thomas Skryabin, Boris V. Rozhdestvensky, Timofey S. Skryabin, Anastasiya B. Brosius, Jürgen
spellingShingle	Robeck, Thomas Skryabin, Boris V. Rozhdestvensky, Timofey S. Skryabin, Anastasiya B. Brosius, Jürgen BC1 RNA motifs required for dendritic transport in vivo
author_facet	Robeck, Thomas Skryabin, Boris V. Rozhdestvensky, Timofey S. Skryabin, Anastasiya B. Brosius, Jürgen
author_sort	Robeck, Thomas
title	BC1 RNA motifs required for dendritic transport in vivo
title_short	BC1 RNA motifs required for dendritic transport in vivo
title_full	BC1 RNA motifs required for dendritic transport in vivo
title_fullStr	BC1 RNA motifs required for dendritic transport in vivo
title_full_unstemmed	BC1 RNA motifs required for dendritic transport in vivo
title_sort	bc1 rna motifs required for dendritic transport in vivo
description	BC1 RNA is a small brain specific non-protein coding RNA. It is transported from the cell body into dendrites where it is involved in the fine-tuning translational control. Due to its compactness and established secondary structure, BC1 RNA is an ideal model for investigating the motifs necessary for dendritic localization. Previously, microinjection of in vitro transcribed BC1 RNA mutants into the soma of cultured primary neurons suggested the importance of RNA motifs for dendritic targeting. These ex vivo experiments identified a single bulged nucleotide (U22) and a putative K-turn (GA motif) structure required for dendritic localization or distal transport, respectively. We generated six transgenic mouse lines (three founders each) containing neuronally expressing BC1 RNA variants on a BC1 RNA knockout mouse background. In contrast to ex vivo data, we did not find indications of reduction or abolition of dendritic BC1 RNA localization in the mutants devoid of the GA motif or the bulged nucleotide. We confirmed the ex vivo data, which showed that the triloop terminal sequence had no consequence on dendritic transport. Interestingly, changing the triloop supporting structure completely abolished dendritic localization of BC1 RNA. We propose a novel RNA motif important for dendritic transport in vivo.
publisher	Nature Publishing Group
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4923876/
_version_	1613600519745437696

BC1 RNA motifs required for dendritic transport in vivo

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