An in vitro assay to study the recruitment and substrate specificity of chromatin modifying enzymes
Post-translational modifications of core histones play an important role in regulating fundamental biological processes such as DNA repair, transcription and replication. In this paper, we describe a novel assay that allows sequential targeting of distinct histone modifying enzymes to immobilized nu...
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| Format: | Online |
| Language: | English |
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Biological Procedures Online
2004
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC491765/ |
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pubmed-4917652004-07-28 An in vitro assay to study the recruitment and substrate specificity of chromatin modifying enzymes Vermeulen, Michiel Stunnenberg, Hendrik G. Research Article Post-translational modifications of core histones play an important role in regulating fundamental biological processes such as DNA repair, transcription and replication. In this paper, we describe a novel assay that allows sequential targeting of distinct histone modifying enzymes to immobilized nucleosomal templates using recombinant chimeric targeting molecules. The assay can be used to study the histone substrate specificity of chromatin modifying enzymes as well as whether and how certain enzymes affect each other's histone modifying activities. As such the assay can help to understand how a certain histone code is established and interpreted. Biological Procedures Online 2004-07-27 /pmc/articles/PMC491765/ /pubmed/15282629 http://dx.doi.org/10.1251/bpo85 Text en Copyright © July 07, 2004, M Vermeulen et al. Published in Biological Procedures Online under license from the authors. Copying, printing, redistribution and storage permitted. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Vermeulen, Michiel Stunnenberg, Hendrik G. |
| spellingShingle |
Vermeulen, Michiel Stunnenberg, Hendrik G. An in vitro assay to study the recruitment and substrate specificity of chromatin modifying enzymes |
| author_facet |
Vermeulen, Michiel Stunnenberg, Hendrik G. |
| author_sort |
Vermeulen, Michiel |
| title |
An in vitro assay to study the recruitment and substrate specificity of chromatin modifying enzymes |
| title_short |
An in vitro assay to study the recruitment and substrate specificity of chromatin modifying enzymes |
| title_full |
An in vitro assay to study the recruitment and substrate specificity of chromatin modifying enzymes |
| title_fullStr |
An in vitro assay to study the recruitment and substrate specificity of chromatin modifying enzymes |
| title_full_unstemmed |
An in vitro assay to study the recruitment and substrate specificity of chromatin modifying enzymes |
| title_sort |
in vitro assay to study the recruitment and substrate specificity of chromatin modifying enzymes |
| description |
Post-translational modifications of core histones play an important role in regulating fundamental biological processes such as DNA repair, transcription and replication. In this paper, we describe a novel assay that allows sequential targeting of distinct histone modifying enzymes to immobilized nucleosomal templates using recombinant chimeric targeting molecules. The assay can be used to study the histone substrate specificity of chromatin modifying enzymes as well as whether and how certain enzymes affect each other's histone modifying activities. As such the assay can help to understand how a certain histone code is established and interpreted. |
| publisher |
Biological Procedures Online |
| publishDate |
2004 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC491765/ |
| _version_ |
1611369139380682752 |