Biodegradation of butyronitrile and demonstration of its mineralization by Rhodococcus sp. MTB5

A nitrile utilizing bacterium Rhodococcus sp. MTB5 was previously isolated in our laboratory by the enrichment culture technique. It is able to utilize butyronitrile as sole carbon, nitrogen, and energy source. Maximum butyronitrile degrading property of this strain has been investigated. Results re...

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Main Authors:	Mukram, Ismailsab, Ramesh, Masarbo, Monisha, T. R., Nayak, Anand S., Karegoudar, T. B.
Format:	Online
Language:	English
Published:	Springer Berlin Heidelberg 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4917499/

id	pubmed-4917499
recordtype	oai_dc
spelling	pubmed-49174992016-06-23 Biodegradation of butyronitrile and demonstration of its mineralization by Rhodococcus sp. MTB5 Mukram, Ismailsab Ramesh, Masarbo Monisha, T. R. Nayak, Anand S. Karegoudar, T. B. Original Article A nitrile utilizing bacterium Rhodococcus sp. MTB5 was previously isolated in our laboratory by the enrichment culture technique. It is able to utilize butyronitrile as sole carbon, nitrogen, and energy source. Maximum butyronitrile degrading property of this strain has been investigated. Results reveal that 100, 98, and 88 % degradation was achieved for 2, 2.5, and 3 % butyronitrile, respectively. The strain is capable of growing in as high as 5 % butyronitrile concentration. A two-step pathway involving nitrile hydratase (NHase) and amidase was observed for the biodegradation of butyronitrile. Complete degradation (mineralization) of butyronitrile with the help of metabolite feeding experiment was reported. The significance of this investigation was the capability of the strain to completely degrade and its ability to grow on higher concentrations of butyronitrile. These potential features make it a suitable candidate for practical field application for effective in situ bioremediation of butyronitrile contaminated sites. Springer Berlin Heidelberg 2016-06-22 2016-12 /pmc/articles/PMC4917499/ /pubmed/28330213 http://dx.doi.org/10.1007/s13205-016-0456-0 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Mukram, Ismailsab Ramesh, Masarbo Monisha, T. R. Nayak, Anand S. Karegoudar, T. B.
spellingShingle	Mukram, Ismailsab Ramesh, Masarbo Monisha, T. R. Nayak, Anand S. Karegoudar, T. B. Biodegradation of butyronitrile and demonstration of its mineralization by Rhodococcus sp. MTB5
author_facet	Mukram, Ismailsab Ramesh, Masarbo Monisha, T. R. Nayak, Anand S. Karegoudar, T. B.
author_sort	Mukram, Ismailsab
title	Biodegradation of butyronitrile and demonstration of its mineralization by Rhodococcus sp. MTB5
title_short	Biodegradation of butyronitrile and demonstration of its mineralization by Rhodococcus sp. MTB5
title_full	Biodegradation of butyronitrile and demonstration of its mineralization by Rhodococcus sp. MTB5
title_fullStr	Biodegradation of butyronitrile and demonstration of its mineralization by Rhodococcus sp. MTB5
title_full_unstemmed	Biodegradation of butyronitrile and demonstration of its mineralization by Rhodococcus sp. MTB5
title_sort	biodegradation of butyronitrile and demonstration of its mineralization by rhodococcus sp. mtb5
description	A nitrile utilizing bacterium Rhodococcus sp. MTB5 was previously isolated in our laboratory by the enrichment culture technique. It is able to utilize butyronitrile as sole carbon, nitrogen, and energy source. Maximum butyronitrile degrading property of this strain has been investigated. Results reveal that 100, 98, and 88 % degradation was achieved for 2, 2.5, and 3 % butyronitrile, respectively. The strain is capable of growing in as high as 5 % butyronitrile concentration. A two-step pathway involving nitrile hydratase (NHase) and amidase was observed for the biodegradation of butyronitrile. Complete degradation (mineralization) of butyronitrile with the help of metabolite feeding experiment was reported. The significance of this investigation was the capability of the strain to completely degrade and its ability to grow on higher concentrations of butyronitrile. These potential features make it a suitable candidate for practical field application for effective in situ bioremediation of butyronitrile contaminated sites.
publisher	Springer Berlin Heidelberg
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4917499/
_version_	1613598301670604800

Biodegradation of butyronitrile and demonstration of its mineralization by Rhodococcus sp. MTB5

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