The induction and identification of novel Colistin resistance mutations in Acinetobacter baumannii and their implications
Acinetobacter baumannii is a significant cause of opportunistic hospital acquired infection and has been identified as an important emerging infection due to its high levels of antimicrobial resistance. Multidrug resistant A. baumannii has risen rapidly in Vietnam, where colistin is becoming the dru...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Online |
| Language: | English |
| Published: |
Nature Publishing Group
2016
|
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4916428/ |
| id |
pubmed-4916428 |
|---|---|
| recordtype |
oai_dc |
| spelling |
pubmed-49164282016-06-27 The induction and identification of novel Colistin resistance mutations in Acinetobacter baumannii and their implications Thi Khanh Nhu, Nguyen Riordan, David W. Do Hoang Nhu, Tran Thanh, Duy Pham Thwaites, Guy Huong Lan, Nguyen Phu Wren, Brendan W. Baker, Stephen Stabler, Richard A Article Acinetobacter baumannii is a significant cause of opportunistic hospital acquired infection and has been identified as an important emerging infection due to its high levels of antimicrobial resistance. Multidrug resistant A. baumannii has risen rapidly in Vietnam, where colistin is becoming the drug of last resort for many infections. In this study we generated spontaneous colistin resistant progeny (up to >256 μg/μl) from four colistin susceptible Vietnamese isolates and one susceptible reference strain (MIC <1.5 μg/μl). Whole genome sequencing was used to identify single nucleotide mutations that could be attributed to the reduced colistin susceptibility. We identified six lpxACD and three pmrB mutations, the majority of which were novel. In addition, we identified further mutations in six A. baumannii genes (vacJ, pldA, ttg2C, pheS and conserved hypothetical protein) that we hypothesise have a role in reduced colistin susceptibility. This study has identified additional mutations that may be associated with colistin resistance through novel resistance mechanisms. Our work further demonstrates how rapidly A. baumannii can generate resistance to a last resort antimicrobial and highlights the need for improved surveillance to identified A. baumannii with an extensive drug resistance profile. Nature Publishing Group 2016-06-22 /pmc/articles/PMC4916428/ /pubmed/27329501 http://dx.doi.org/10.1038/srep28291 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Thi Khanh Nhu, Nguyen Riordan, David W. Do Hoang Nhu, Tran Thanh, Duy Pham Thwaites, Guy Huong Lan, Nguyen Phu Wren, Brendan W. Baker, Stephen Stabler, Richard A |
| spellingShingle |
Thi Khanh Nhu, Nguyen Riordan, David W. Do Hoang Nhu, Tran Thanh, Duy Pham Thwaites, Guy Huong Lan, Nguyen Phu Wren, Brendan W. Baker, Stephen Stabler, Richard A The induction and identification of novel Colistin resistance mutations in Acinetobacter baumannii and their implications |
| author_facet |
Thi Khanh Nhu, Nguyen Riordan, David W. Do Hoang Nhu, Tran Thanh, Duy Pham Thwaites, Guy Huong Lan, Nguyen Phu Wren, Brendan W. Baker, Stephen Stabler, Richard A |
| author_sort |
Thi Khanh Nhu, Nguyen |
| title |
The induction and identification of novel Colistin resistance mutations in Acinetobacter baumannii and their implications |
| title_short |
The induction and identification of novel Colistin resistance mutations in Acinetobacter baumannii and their implications |
| title_full |
The induction and identification of novel Colistin resistance mutations in Acinetobacter baumannii and their implications |
| title_fullStr |
The induction and identification of novel Colistin resistance mutations in Acinetobacter baumannii and their implications |
| title_full_unstemmed |
The induction and identification of novel Colistin resistance mutations in Acinetobacter baumannii and their implications |
| title_sort |
induction and identification of novel colistin resistance mutations in acinetobacter baumannii and their implications |
| description |
Acinetobacter baumannii is a significant cause of opportunistic hospital acquired infection and has been identified as an important emerging infection due to its high levels of antimicrobial resistance. Multidrug resistant A. baumannii has risen rapidly in Vietnam, where colistin is becoming the drug of last resort for many infections. In this study we generated spontaneous colistin resistant progeny (up to >256 μg/μl) from four colistin susceptible Vietnamese isolates and one susceptible reference strain (MIC <1.5 μg/μl). Whole genome sequencing was used to identify single nucleotide mutations that could be attributed to the reduced colistin susceptibility. We identified six lpxACD and three pmrB mutations, the majority of which were novel. In addition, we identified further mutations in six A. baumannii genes (vacJ, pldA, ttg2C, pheS and conserved hypothetical protein) that we hypothesise have a role in reduced colistin susceptibility. This study has identified additional mutations that may be associated with colistin resistance through novel resistance mechanisms. Our work further demonstrates how rapidly A. baumannii can generate resistance to a last resort antimicrobial and highlights the need for improved surveillance to identified A. baumannii with an extensive drug resistance profile. |
| publisher |
Nature Publishing Group |
| publishDate |
2016 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4916428/ |
| _version_ |
1613597976384503808 |