MiR-367 negatively regulates apoptosis induced by adriamycin in osteosarcoma cells by targeting KLF4

Diverse functions of microRNAs have been investigated in tumorigenesis in osteosarcoma (OS), involving the regulation of proliferation, invasion, migration, apoptosis and drug resistance. MiR-367 was found to be an oncogene and increased in OS. However, the function of miR-367 in drug resistance in...

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Main Authors:	Wang, Guang-Chao, He, Qian-Yun, Tong, Da-Ke, Wang, Chuan-Feng, Liu, Kang, Ding, Chen, Ji, Fang, Zhang, Hao
Format:	Online
Language:	English
Published:	Elsevier 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4908187/

id	pubmed-4908187
recordtype	oai_dc
spelling	pubmed-49081872016-06-22 MiR-367 negatively regulates apoptosis induced by adriamycin in osteosarcoma cells by targeting KLF4 Wang, Guang-Chao He, Qian-Yun Tong, Da-Ke Wang, Chuan-Feng Liu, Kang Ding, Chen Ji, Fang Zhang, Hao Research Paper Diverse functions of microRNAs have been investigated in tumorigenesis in osteosarcoma (OS), involving the regulation of proliferation, invasion, migration, apoptosis and drug resistance. MiR-367 was found to be an oncogene and increased in OS. However, the function of miR-367 in drug resistance in OS cells is still unknown. In this study, we found that miR-367 was up-regulated in OS tissues and OS cell cultures. Meanwhile, treatment with adriamycin (ADR) induced apoptosis of OS cells with upregulation of miR-367. Notably, KLF4 was demonstrated to be a direct target of miR-367 by gene reporter assay, and miR-367 significantly blocked both mRNA and protein level of KLF4. In addition, overexpression of miR-367 markedly suppressed the increase of KLF4 induced by ADR in OS cells, as well as Bax and cleaved caspase-3, which were significantly reversed by anti-miR-367 transfection. Taken together, our data demonstrates that miR-367 and KLF4 play important roles in OS treatment and ADR resistance, suggesting that miR-367 is a potential biomarker of chemotherapy resistance in OS and also probably a novel therapeutic target against OS. Elsevier 2016-02-17 /pmc/articles/PMC4908187/ /pubmed/27335771 http://dx.doi.org/10.1016/j.jbo.2016.02.002 Text en © 2016 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Wang, Guang-Chao He, Qian-Yun Tong, Da-Ke Wang, Chuan-Feng Liu, Kang Ding, Chen Ji, Fang Zhang, Hao
spellingShingle	Wang, Guang-Chao He, Qian-Yun Tong, Da-Ke Wang, Chuan-Feng Liu, Kang Ding, Chen Ji, Fang Zhang, Hao MiR-367 negatively regulates apoptosis induced by adriamycin in osteosarcoma cells by targeting KLF4
author_facet	Wang, Guang-Chao He, Qian-Yun Tong, Da-Ke Wang, Chuan-Feng Liu, Kang Ding, Chen Ji, Fang Zhang, Hao
author_sort	Wang, Guang-Chao
title	MiR-367 negatively regulates apoptosis induced by adriamycin in osteosarcoma cells by targeting KLF4
title_short	MiR-367 negatively regulates apoptosis induced by adriamycin in osteosarcoma cells by targeting KLF4
title_full	MiR-367 negatively regulates apoptosis induced by adriamycin in osteosarcoma cells by targeting KLF4
title_fullStr	MiR-367 negatively regulates apoptosis induced by adriamycin in osteosarcoma cells by targeting KLF4
title_full_unstemmed	MiR-367 negatively regulates apoptosis induced by adriamycin in osteosarcoma cells by targeting KLF4
title_sort	mir-367 negatively regulates apoptosis induced by adriamycin in osteosarcoma cells by targeting klf4
description	Diverse functions of microRNAs have been investigated in tumorigenesis in osteosarcoma (OS), involving the regulation of proliferation, invasion, migration, apoptosis and drug resistance. MiR-367 was found to be an oncogene and increased in OS. However, the function of miR-367 in drug resistance in OS cells is still unknown. In this study, we found that miR-367 was up-regulated in OS tissues and OS cell cultures. Meanwhile, treatment with adriamycin (ADR) induced apoptosis of OS cells with upregulation of miR-367. Notably, KLF4 was demonstrated to be a direct target of miR-367 by gene reporter assay, and miR-367 significantly blocked both mRNA and protein level of KLF4. In addition, overexpression of miR-367 markedly suppressed the increase of KLF4 induced by ADR in OS cells, as well as Bax and cleaved caspase-3, which were significantly reversed by anti-miR-367 transfection. Taken together, our data demonstrates that miR-367 and KLF4 play important roles in OS treatment and ADR resistance, suggesting that miR-367 is a potential biomarker of chemotherapy resistance in OS and also probably a novel therapeutic target against OS.
publisher	Elsevier
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4908187/
_version_	1613594766221508608

MiR-367 negatively regulates apoptosis induced by adriamycin in osteosarcoma cells by targeting KLF4

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