Identification and tissue expression profile of genes from three chemoreceptor families in an urban pest, Periplaneta americana

Periplaneta americana is a notorious urban pest prevalent in human habitats; very little is known about its chemosensory mechanism. Employing the advanced next-generation sequencing technique, in the present study, we conducted transcriptome sequencing and analysis of the antennae of the adult males...

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Main Authors:	Chen, Yan, He, Ming, Li, Zhao-Qun, Zhang, Ya-Nan, He, Peng
Format:	Online
Language:	English
Published:	Nature Publishing Group 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4899716/

id	pubmed-4899716
recordtype	oai_dc
spelling	pubmed-48997162016-06-13 Identification and tissue expression profile of genes from three chemoreceptor families in an urban pest, Periplaneta americana Chen, Yan He, Ming Li, Zhao-Qun Zhang, Ya-Nan He, Peng Article Periplaneta americana is a notorious urban pest prevalent in human habitats; very little is known about its chemosensory mechanism. Employing the advanced next-generation sequencing technique, in the present study, we conducted transcriptome sequencing and analysis of the antennae of the adult males and females as well as their mouthparts using an Illumina platform. This resulted in the discovery of a huge number of the members of all major known chemosensory receptor families in P. americana, including 96 odorant receptors (ORs), 53 ionotropic receptors (IRs), and 33 gustatory receptors (GRs). Tissue expression profiles showed most of them mainly expressed in antennae and phylogenetic analysis demonstrated the expansion in the clade distinguishing them from other functionally well-known Lepidoptera species. A high percentage of chemosensory receptor genes (ORs in particular) showing female antenna bias in mRNA expression was observed. Our results provide a basis for further investigations on how P. americana coordinates its chemosensory receptor genes in chemical communication with environments, and for development of novel pest management approaches. Nature Publishing Group 2016-06-09 /pmc/articles/PMC4899716/ /pubmed/27279336 http://dx.doi.org/10.1038/srep27495 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Chen, Yan He, Ming Li, Zhao-Qun Zhang, Ya-Nan He, Peng
spellingShingle	Chen, Yan He, Ming Li, Zhao-Qun Zhang, Ya-Nan He, Peng Identification and tissue expression profile of genes from three chemoreceptor families in an urban pest, Periplaneta americana
author_facet	Chen, Yan He, Ming Li, Zhao-Qun Zhang, Ya-Nan He, Peng
author_sort	Chen, Yan
title	Identification and tissue expression profile of genes from three chemoreceptor families in an urban pest, Periplaneta americana
title_short	Identification and tissue expression profile of genes from three chemoreceptor families in an urban pest, Periplaneta americana
title_full	Identification and tissue expression profile of genes from three chemoreceptor families in an urban pest, Periplaneta americana
title_fullStr	Identification and tissue expression profile of genes from three chemoreceptor families in an urban pest, Periplaneta americana
title_full_unstemmed	Identification and tissue expression profile of genes from three chemoreceptor families in an urban pest, Periplaneta americana
title_sort	identification and tissue expression profile of genes from three chemoreceptor families in an urban pest, periplaneta americana
description	Periplaneta americana is a notorious urban pest prevalent in human habitats; very little is known about its chemosensory mechanism. Employing the advanced next-generation sequencing technique, in the present study, we conducted transcriptome sequencing and analysis of the antennae of the adult males and females as well as their mouthparts using an Illumina platform. This resulted in the discovery of a huge number of the members of all major known chemosensory receptor families in P. americana, including 96 odorant receptors (ORs), 53 ionotropic receptors (IRs), and 33 gustatory receptors (GRs). Tissue expression profiles showed most of them mainly expressed in antennae and phylogenetic analysis demonstrated the expansion in the clade distinguishing them from other functionally well-known Lepidoptera species. A high percentage of chemosensory receptor genes (ORs in particular) showing female antenna bias in mRNA expression was observed. Our results provide a basis for further investigations on how P. americana coordinates its chemosensory receptor genes in chemical communication with environments, and for development of novel pest management approaches.
publisher	Nature Publishing Group
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4899716/
_version_	1613591685285019648

Identification and tissue expression profile of genes from three chemoreceptor families in an urban pest, Periplaneta americana

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