Immune Protection of Retroviral Vectors Upon Molecular Painting with the Complement Regulatory Protein CD59

Glycosylphosphatidylinositol anchoring is a type of post-translational modification that allows proteins to be presented on the exterior side of the cell membrane. Purified glycosylphosphatidylinositol-anchored protein can spontaneously re-insert into lipid bilayer membranes in a process termed Mole...

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Main Authors:	Heider, Susanne, Kleinberger, Sandra, Kochan, Feliks, Dangerfield, John A., Metzner, Christoph
Format:	Online
Language:	English
Published:	Springer US 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4899500/

id	pubmed-4899500
recordtype	oai_dc
spelling	pubmed-48995002016-06-27 Immune Protection of Retroviral Vectors Upon Molecular Painting with the Complement Regulatory Protein CD59 Heider, Susanne Kleinberger, Sandra Kochan, Feliks Dangerfield, John A. Metzner, Christoph Original Paper Glycosylphosphatidylinositol anchoring is a type of post-translational modification that allows proteins to be presented on the exterior side of the cell membrane. Purified glycosylphosphatidylinositol-anchored protein can spontaneously re-insert into lipid bilayer membranes in a process termed Molecular Painting. Here, we demonstrate the possibility of inserting purified, recombinant CD59 into virus particles produced from a murine retroviral producer cell line. CD59 is a regulator of the complement system that helps protect healthy cells from the lytic activity of the complement cascade. In this study, we could show that Molecular Painting confers protection from complement activity upon murine retroviral vector particles. Indeed, increased infectivity of CD59-modified virus particles was observed upon challenge with human serum, indicating that Molecular Painting is suitable for modulating the immune system in gene therapy or vaccination applications. Springer US 2016-05-11 2016 /pmc/articles/PMC4899500/ /pubmed/27170144 http://dx.doi.org/10.1007/s12033-016-9944-z Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Heider, Susanne Kleinberger, Sandra Kochan, Feliks Dangerfield, John A. Metzner, Christoph
spellingShingle	Heider, Susanne Kleinberger, Sandra Kochan, Feliks Dangerfield, John A. Metzner, Christoph Immune Protection of Retroviral Vectors Upon Molecular Painting with the Complement Regulatory Protein CD59
author_facet	Heider, Susanne Kleinberger, Sandra Kochan, Feliks Dangerfield, John A. Metzner, Christoph
author_sort	Heider, Susanne
title	Immune Protection of Retroviral Vectors Upon Molecular Painting with the Complement Regulatory Protein CD59
title_short	Immune Protection of Retroviral Vectors Upon Molecular Painting with the Complement Regulatory Protein CD59
title_full	Immune Protection of Retroviral Vectors Upon Molecular Painting with the Complement Regulatory Protein CD59
title_fullStr	Immune Protection of Retroviral Vectors Upon Molecular Painting with the Complement Regulatory Protein CD59
title_full_unstemmed	Immune Protection of Retroviral Vectors Upon Molecular Painting with the Complement Regulatory Protein CD59
title_sort	immune protection of retroviral vectors upon molecular painting with the complement regulatory protein cd59
description	Glycosylphosphatidylinositol anchoring is a type of post-translational modification that allows proteins to be presented on the exterior side of the cell membrane. Purified glycosylphosphatidylinositol-anchored protein can spontaneously re-insert into lipid bilayer membranes in a process termed Molecular Painting. Here, we demonstrate the possibility of inserting purified, recombinant CD59 into virus particles produced from a murine retroviral producer cell line. CD59 is a regulator of the complement system that helps protect healthy cells from the lytic activity of the complement cascade. In this study, we could show that Molecular Painting confers protection from complement activity upon murine retroviral vector particles. Indeed, increased infectivity of CD59-modified virus particles was observed upon challenge with human serum, indicating that Molecular Painting is suitable for modulating the immune system in gene therapy or vaccination applications.
publisher	Springer US
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4899500/
_version_	1613591561095872512

Immune Protection of Retroviral Vectors Upon Molecular Painting with the Complement Regulatory Protein CD59

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