Sortilin is associated with the chlamydial inclusion and is modulated during infection

Chlamydia species are obligate intracellular pathogens that have a major impact on human health. The pathogen replicates within an intracellular niche called an inclusion and is thought to rely heavily on host-derived proteins and lipids, including ceramide. Sortilin is a transmembrane receptor impl...

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Main Authors:	Teo, Wei Xuan, Kerr, Markus Charles, Huston, Wilhelmina May, Teasdale, Rohan David
Format:	Online
Language:	English
Published:	The Company of Biologists Ltd 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4890668/

id	pubmed-4890668
recordtype	oai_dc
spelling	pubmed-48906682016-06-03 Sortilin is associated with the chlamydial inclusion and is modulated during infection Teo, Wei Xuan Kerr, Markus Charles Huston, Wilhelmina May Teasdale, Rohan David Research Article Chlamydia species are obligate intracellular pathogens that have a major impact on human health. The pathogen replicates within an intracellular niche called an inclusion and is thought to rely heavily on host-derived proteins and lipids, including ceramide. Sortilin is a transmembrane receptor implicated in the trafficking of acid sphingomyelinase, which is responsible for catalysing the breakdown of sphingomyelin to ceramide. In this study, we examined the role of sortilin in Chlamydia trachomatis L2 development. Western immunoblotting and immunocytochemistry analysis revealed that endogenous sortilin is not only associated with the inclusion, but that protein levels increase in infected cells. RNAi-mediated depletion of sortilin, however, had no detectable impact on ceramide delivery to the inclusion or the production of infectious progeny. This study demonstrates that whilst Chlamydia redirects sortilin trafficking to the chlamydial inclusion, RNAi knockdown of sortilin expression is insufficient to determine if this pathway is requisite for the development of the pathogen. The Company of Biologists Ltd 2016-03-09 /pmc/articles/PMC4890668/ /pubmed/26962046 http://dx.doi.org/10.1242/bio.016485 Text en © 2016. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Teo, Wei Xuan Kerr, Markus Charles Huston, Wilhelmina May Teasdale, Rohan David
spellingShingle	Teo, Wei Xuan Kerr, Markus Charles Huston, Wilhelmina May Teasdale, Rohan David Sortilin is associated with the chlamydial inclusion and is modulated during infection
author_facet	Teo, Wei Xuan Kerr, Markus Charles Huston, Wilhelmina May Teasdale, Rohan David
author_sort	Teo, Wei Xuan
title	Sortilin is associated with the chlamydial inclusion and is modulated during infection
title_short	Sortilin is associated with the chlamydial inclusion and is modulated during infection
title_full	Sortilin is associated with the chlamydial inclusion and is modulated during infection
title_fullStr	Sortilin is associated with the chlamydial inclusion and is modulated during infection
title_full_unstemmed	Sortilin is associated with the chlamydial inclusion and is modulated during infection
title_sort	sortilin is associated with the chlamydial inclusion and is modulated during infection
description	Chlamydia species are obligate intracellular pathogens that have a major impact on human health. The pathogen replicates within an intracellular niche called an inclusion and is thought to rely heavily on host-derived proteins and lipids, including ceramide. Sortilin is a transmembrane receptor implicated in the trafficking of acid sphingomyelinase, which is responsible for catalysing the breakdown of sphingomyelin to ceramide. In this study, we examined the role of sortilin in Chlamydia trachomatis L2 development. Western immunoblotting and immunocytochemistry analysis revealed that endogenous sortilin is not only associated with the inclusion, but that protein levels increase in infected cells. RNAi-mediated depletion of sortilin, however, had no detectable impact on ceramide delivery to the inclusion or the production of infectious progeny. This study demonstrates that whilst Chlamydia redirects sortilin trafficking to the chlamydial inclusion, RNAi knockdown of sortilin expression is insufficient to determine if this pathway is requisite for the development of the pathogen.
publisher	The Company of Biologists Ltd
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4890668/
_version_	1613587846008930304

Sortilin is associated with the chlamydial inclusion and is modulated during infection

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