De novo assembly and characterization of the Chinese three-keeled pond turtle (Mauremys reevesii) transcriptome: presence of longevity-related genes

Mauremys reevesii (Geoemydidae) is one of the most common and widespread semi-aquatic turtles in East Asia. The unusually long lifespan of some individuals makes this turtle species a potentially useful model organism for studying the molecular basis of longevity. In this study, pooled total RNA ext...

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Main Authors:	Yin, Huazong, Nie, Liuwang, Zhao, Feifei, Zhou, Huaxing, Li, Haifeng, Dong, Xianmei, Zhang, Huanhuan, Wang, Yuqin, Shi, Qiong, Li, Jun
Format:	Online
Language:	English
Published:	PeerJ Inc. 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4888314/

id	pubmed-4888314
recordtype	oai_dc
spelling	pubmed-48883142016-06-02 De novo assembly and characterization of the Chinese three-keeled pond turtle (Mauremys reevesii) transcriptome: presence of longevity-related genes Yin, Huazong Nie, Liuwang Zhao, Feifei Zhou, Huaxing Li, Haifeng Dong, Xianmei Zhang, Huanhuan Wang, Yuqin Shi, Qiong Li, Jun Genetics Mauremys reevesii (Geoemydidae) is one of the most common and widespread semi-aquatic turtles in East Asia. The unusually long lifespan of some individuals makes this turtle species a potentially useful model organism for studying the molecular basis of longevity. In this study, pooled total RNA extracted from liver, spleen and skeletal-muscle of three adult individuals were sequenced using Illumina Hiseq 2500 platform. A set of telomere-related genes were found in the transcriptome, including tert, tep1, and six shelterin complex proteins coding genes (trf1, trf2, tpp1, pot1, tin2 and rap1). These genes products protect chromosome ends from deterioration and therefore significantly contribute to turtle longevity. The transcriptome data generated in this study provides a comprehensive reference for future molecular studies in the turtle. PeerJ Inc. 2016-05-24 /pmc/articles/PMC4888314/ /pubmed/27257545 http://dx.doi.org/10.7717/peerj.2062 Text en ©2016 Yin et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Yin, Huazong Nie, Liuwang Zhao, Feifei Zhou, Huaxing Li, Haifeng Dong, Xianmei Zhang, Huanhuan Wang, Yuqin Shi, Qiong Li, Jun
spellingShingle	Yin, Huazong Nie, Liuwang Zhao, Feifei Zhou, Huaxing Li, Haifeng Dong, Xianmei Zhang, Huanhuan Wang, Yuqin Shi, Qiong Li, Jun De novo assembly and characterization of the Chinese three-keeled pond turtle (Mauremys reevesii) transcriptome: presence of longevity-related genes
author_facet	Yin, Huazong Nie, Liuwang Zhao, Feifei Zhou, Huaxing Li, Haifeng Dong, Xianmei Zhang, Huanhuan Wang, Yuqin Shi, Qiong Li, Jun
author_sort	Yin, Huazong
title	De novo assembly and characterization of the Chinese three-keeled pond turtle (Mauremys reevesii) transcriptome: presence of longevity-related genes
title_short	De novo assembly and characterization of the Chinese three-keeled pond turtle (Mauremys reevesii) transcriptome: presence of longevity-related genes
title_full	De novo assembly and characterization of the Chinese three-keeled pond turtle (Mauremys reevesii) transcriptome: presence of longevity-related genes
title_fullStr	De novo assembly and characterization of the Chinese three-keeled pond turtle (Mauremys reevesii) transcriptome: presence of longevity-related genes
title_full_unstemmed	De novo assembly and characterization of the Chinese three-keeled pond turtle (Mauremys reevesii) transcriptome: presence of longevity-related genes
title_sort	de novo assembly and characterization of the chinese three-keeled pond turtle (mauremys reevesii) transcriptome: presence of longevity-related genes
description	Mauremys reevesii (Geoemydidae) is one of the most common and widespread semi-aquatic turtles in East Asia. The unusually long lifespan of some individuals makes this turtle species a potentially useful model organism for studying the molecular basis of longevity. In this study, pooled total RNA extracted from liver, spleen and skeletal-muscle of three adult individuals were sequenced using Illumina Hiseq 2500 platform. A set of telomere-related genes were found in the transcriptome, including tert, tep1, and six shelterin complex proteins coding genes (trf1, trf2, tpp1, pot1, tin2 and rap1). These genes products protect chromosome ends from deterioration and therefore significantly contribute to turtle longevity. The transcriptome data generated in this study provides a comprehensive reference for future molecular studies in the turtle.
publisher	PeerJ Inc.
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4888314/
_version_	1613587064360534016

De novo assembly and characterization of the Chinese three-keeled pond turtle (Mauremys reevesii) transcriptome: presence of longevity-related genes

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