Comprehensive and computational analysis of genes in human umbilical vein endothelial cells responsive to X-irradiation

Radiation exposure such as A-bomb or radiation therapy is considered a major health-risk factor for cardiovascular disease. In order to understand the molecular mechanisms underlying the inflammatory reaction frequently encountered in the vascular system after exposure to ionizing radiation, we carr...

Full description

Bibliographic Details
Main Authors:	Furusawa, Yukihiro, Zhao, Qing-Li, Hattori, Yuichi, Tabuchi, Yoshiaki, Iwasaki, Toshiyasu, Nomura, Takaharu, Kondo, Takashi
Format:	Online
Language:	English
Published:	Elsevier 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4880795/

id	pubmed-4880795
recordtype	oai_dc
spelling	pubmed-48807952016-06-06 Comprehensive and computational analysis of genes in human umbilical vein endothelial cells responsive to X-irradiation Furusawa, Yukihiro Zhao, Qing-Li Hattori, Yuichi Tabuchi, Yoshiaki Iwasaki, Toshiyasu Nomura, Takaharu Kondo, Takashi Regular Article Radiation exposure such as A-bomb or radiation therapy is considered a major health-risk factor for cardiovascular disease. In order to understand the molecular mechanisms underlying the inflammatory reaction frequently encountered in the vascular system after exposure to ionizing radiation, we carried out a global scale microarray and computational gene expression analyses on human umbilical endothelial cells (HUVECs) exposed to X-ray (2.5 Gy). The gene ontology analysis revealed that the down-regulated genes were associated with cell cycle regulation, whereas the up-regulated genes were associated with inflammatory responses, in particular, the type 1 interferon response. The computational analysis using ingenuity pathway analysis also identified a gene network containing the interferon response factor 7 (IRF7) and its transcriptional targets such as interferon-induced transcripts (IFITs) and Mx1, which have been known to be associated with inflammation in endothelial cells. The up-regulated genes and the gene network identified here may explain the inflammatory response induced by X-irradiation. These findings uncover part of the molecular basis of the mechanism(s) of the inflammatory disorder in response to X-irradiation in HUVECs. The dataset is publicly available at the Gene Expression Omnibus (GEO) repository (http://www.ncbi.nlm.nih.gov/geo/) with accession number GSE76484. Elsevier 2016-05-16 /pmc/articles/PMC4880795/ /pubmed/27275413 http://dx.doi.org/10.1016/j.gdata.2016.05.007 Text en © 2016 The Authors. Published by Elsevier Inc. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Furusawa, Yukihiro Zhao, Qing-Li Hattori, Yuichi Tabuchi, Yoshiaki Iwasaki, Toshiyasu Nomura, Takaharu Kondo, Takashi
spellingShingle	Furusawa, Yukihiro Zhao, Qing-Li Hattori, Yuichi Tabuchi, Yoshiaki Iwasaki, Toshiyasu Nomura, Takaharu Kondo, Takashi Comprehensive and computational analysis of genes in human umbilical vein endothelial cells responsive to X-irradiation
author_facet	Furusawa, Yukihiro Zhao, Qing-Li Hattori, Yuichi Tabuchi, Yoshiaki Iwasaki, Toshiyasu Nomura, Takaharu Kondo, Takashi
author_sort	Furusawa, Yukihiro
title	Comprehensive and computational analysis of genes in human umbilical vein endothelial cells responsive to X-irradiation
title_short	Comprehensive and computational analysis of genes in human umbilical vein endothelial cells responsive to X-irradiation
title_full	Comprehensive and computational analysis of genes in human umbilical vein endothelial cells responsive to X-irradiation
title_fullStr	Comprehensive and computational analysis of genes in human umbilical vein endothelial cells responsive to X-irradiation
title_full_unstemmed	Comprehensive and computational analysis of genes in human umbilical vein endothelial cells responsive to X-irradiation
title_sort	comprehensive and computational analysis of genes in human umbilical vein endothelial cells responsive to x-irradiation
description	Radiation exposure such as A-bomb or radiation therapy is considered a major health-risk factor for cardiovascular disease. In order to understand the molecular mechanisms underlying the inflammatory reaction frequently encountered in the vascular system after exposure to ionizing radiation, we carried out a global scale microarray and computational gene expression analyses on human umbilical endothelial cells (HUVECs) exposed to X-ray (2.5 Gy). The gene ontology analysis revealed that the down-regulated genes were associated with cell cycle regulation, whereas the up-regulated genes were associated with inflammatory responses, in particular, the type 1 interferon response. The computational analysis using ingenuity pathway analysis also identified a gene network containing the interferon response factor 7 (IRF7) and its transcriptional targets such as interferon-induced transcripts (IFITs) and Mx1, which have been known to be associated with inflammation in endothelial cells. The up-regulated genes and the gene network identified here may explain the inflammatory response induced by X-irradiation. These findings uncover part of the molecular basis of the mechanism(s) of the inflammatory disorder in response to X-irradiation in HUVECs. The dataset is publicly available at the Gene Expression Omnibus (GEO) repository (http://www.ncbi.nlm.nih.gov/geo/) with accession number GSE76484.
publisher	Elsevier
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4880795/
_version_	1613584320691175424

Comprehensive and computational analysis of genes in human umbilical vein endothelial cells responsive to X-irradiation

Similar Items