Transfection of insect cell in suspension for efficient baculovirus generation

Baculovirus (BV) mediated insect cell expression system utilizes transfection as a first step to introduce recombinant baculovirus DNA into insect cells. Many labs are still relying on the conventional liposome based transfection method in adherent culture. Here we describe a more efficient method t...

Full description

Bibliographic Details
Main Authors: Roest, S., Kapps-Fouthier, S., Klopp, J., Rieffel, S., Gerhartz, B., Shrestha, B.
Format: Online
Language:English
Published: Elsevier 2016
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4872675/