Decreased TPD52 expression is associated with poor prognosis in primary hepatocellular carcinoma
Tumor protein D52 (TPD52) has been indicated to be involved in tumorigenesis of various malignancies. But its role in hepatocellular carcinoma (HCC) is unknown. This study aimed to explore the expression of TPD52 in HCC samples and cell lines using real-time quantitative PCR, western blotting, and i...
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Impact Journals LLC
2015
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4868759/ |
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pubmed-4868759 |
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pubmed-48687592016-05-20 Decreased TPD52 expression is associated with poor prognosis in primary hepatocellular carcinoma Wang, Ying Chen, Chang-Long Pan, Qiu-Zhong Wu, Ying-Yuan Zhao, Jing-Jing Jiang, Shan-Shan Chao, Jie Zhang, Xiao-Fei Zhang, Hong-Xia Zhou, Zi-Qi Tang, Yan Huang, Xu-Qiong Zhang, Jian-Hua Xia, Jian-Chuan Clinical Research Paper Tumor protein D52 (TPD52) has been indicated to be involved in tumorigenesis of various malignancies. But its role in hepatocellular carcinoma (HCC) is unknown. This study aimed to explore the expression of TPD52 in HCC samples and cell lines using real-time quantitative PCR, western blotting, and immunohistochemistry. The prognostic value of TPD52 in HCC was also analysed. Meanwhile, the mechanism of TPD52 in hepatocarcinogenesis was further investigated by western blotting, immunohistochemistry, over-express and knockdown studies. We found that TPD52 expression was significantly decreased in the HCC tissues and HCC cell lines. TPD52 expression was significantly correlated with tumor-nodes-metastasis (TNM) stage. Kaplan–Meier survival curves showed that high TPD52 expression was associated with improved overall survival (OS) and disease-free survival (DFS) in HCC patients. Multivariate analysis indicated that TPD52 expression was an independent prognostic marker for the OS and DFS of patients. In addition, TPD52 expression was positively correlated with p21 and p53 expression, and was negatively correlated with MDM2, BCL2 and P-GSK-3β expression in HCC. In conclusions, our findings suggested that TPD52 is a potential tumor suppressor in HCC. It may be a novel prognostic biomarker and molecular therapy target for HCC. Impact Journals LLC 2015-11-13 /pmc/articles/PMC4868759/ /pubmed/26575170 http://dx.doi.org/10.18632/oncotarget.6319 Text en Copyright: © 2016 Wang et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Wang, Ying Chen, Chang-Long Pan, Qiu-Zhong Wu, Ying-Yuan Zhao, Jing-Jing Jiang, Shan-Shan Chao, Jie Zhang, Xiao-Fei Zhang, Hong-Xia Zhou, Zi-Qi Tang, Yan Huang, Xu-Qiong Zhang, Jian-Hua Xia, Jian-Chuan |
| spellingShingle |
Wang, Ying Chen, Chang-Long Pan, Qiu-Zhong Wu, Ying-Yuan Zhao, Jing-Jing Jiang, Shan-Shan Chao, Jie Zhang, Xiao-Fei Zhang, Hong-Xia Zhou, Zi-Qi Tang, Yan Huang, Xu-Qiong Zhang, Jian-Hua Xia, Jian-Chuan Decreased TPD52 expression is associated with poor prognosis in primary hepatocellular carcinoma |
| author_facet |
Wang, Ying Chen, Chang-Long Pan, Qiu-Zhong Wu, Ying-Yuan Zhao, Jing-Jing Jiang, Shan-Shan Chao, Jie Zhang, Xiao-Fei Zhang, Hong-Xia Zhou, Zi-Qi Tang, Yan Huang, Xu-Qiong Zhang, Jian-Hua Xia, Jian-Chuan |
| author_sort |
Wang, Ying |
| title |
Decreased TPD52 expression is associated with poor prognosis in primary hepatocellular carcinoma |
| title_short |
Decreased TPD52 expression is associated with poor prognosis in primary hepatocellular carcinoma |
| title_full |
Decreased TPD52 expression is associated with poor prognosis in primary hepatocellular carcinoma |
| title_fullStr |
Decreased TPD52 expression is associated with poor prognosis in primary hepatocellular carcinoma |
| title_full_unstemmed |
Decreased TPD52 expression is associated with poor prognosis in primary hepatocellular carcinoma |
| title_sort |
decreased tpd52 expression is associated with poor prognosis in primary hepatocellular carcinoma |
| description |
Tumor protein D52 (TPD52) has been indicated to be involved in tumorigenesis of various malignancies. But its role in hepatocellular carcinoma (HCC) is unknown. This study aimed to explore the expression of TPD52 in HCC samples and cell lines using real-time quantitative PCR, western blotting, and immunohistochemistry. The prognostic value of TPD52 in HCC was also analysed. Meanwhile, the mechanism of TPD52 in hepatocarcinogenesis was further investigated by western blotting, immunohistochemistry, over-express and knockdown studies. We found that TPD52 expression was significantly decreased in the HCC tissues and HCC cell lines. TPD52 expression was significantly correlated with tumor-nodes-metastasis (TNM) stage. Kaplan–Meier survival curves showed that high TPD52 expression was associated with improved overall survival (OS) and disease-free survival (DFS) in HCC patients. Multivariate analysis indicated that TPD52 expression was an independent prognostic marker for the OS and DFS of patients. In addition, TPD52 expression was positively correlated with p21 and p53 expression, and was negatively correlated with MDM2, BCL2 and P-GSK-3β expression in HCC. In conclusions, our findings suggested that TPD52 is a potential tumor suppressor in HCC. It may be a novel prognostic biomarker and molecular therapy target for HCC. |
| publisher |
Impact Journals LLC |
| publishDate |
2015 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4868759/ |
| _version_ |
1613579908069457920 |