SOD2 deregulation enhances migration, invasion and has poor prognosis in salivary adenoid cystic carcinoma

This study aimed to investigate the role of SOD2 in the progression and metastasis of salivary adenoid cystic carcinoma (SACC). We analyzed the expression of SOD2 in 50 SACC patients. Then, the effects and mechanism of SOD2 on cell metastasis in a pair of different metastatic potential cell lines wa...

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Main Authors:	Chang, Boyang, Yang, Hang, Jiao, Yuan, Wang, Kefeng, Liu, Zhonghua, Wu, Peihong, Li, Su, Wang, Anxun
Format:	Online
Language:	English
Published:	Nature Publishing Group 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4867643/

id	pubmed-4867643
recordtype	oai_dc
spelling	pubmed-48676432016-05-31 SOD2 deregulation enhances migration, invasion and has poor prognosis in salivary adenoid cystic carcinoma Chang, Boyang Yang, Hang Jiao, Yuan Wang, Kefeng Liu, Zhonghua Wu, Peihong Li, Su Wang, Anxun Article This study aimed to investigate the role of SOD2 in the progression and metastasis of salivary adenoid cystic carcinoma (SACC). We analyzed the expression of SOD2 in 50 SACC patients. Then, the effects and mechanism of SOD2 on cell metastasis in a pair of different metastatic potential cell lines was investigated. SOD2 was deregulated in patients with SACC. Up-regulation of SOD2 was associated with distant metastasis and reduced overall survival and disease free - survival. Compared to SACC-83 cells (lower metastasis ability), SACC-LM cells (higher metastasis ability) had higher SOD2 activity and intracellular H2O2 concentrations, and protein levels of pERK1/2 and Slug, but had similar catalase protein level and activity. In SACC-LM, reducing the expression of SOD2 by SiRNA inhibited the metastasis ability and reduced the SOD2 activities, intracellular H2O2 concentrations, and protein levels of pERK1/2 and Slug. These effects were revised in SACC-83 after SOD2 overexpression. Moreover, in SACC-83, treated with H2O2, the metastasis was enhanced accompanied by increased protein levels of pERK1/2 and Slug. We confirmed that SOD2 play an important role in the development and prognosis of SACC and SOD2-dependent production of H2O2 contributes to metastasis of SACC through the ERK-Slug signaling pathway. Nature Publishing Group 2016-05-16 /pmc/articles/PMC4867643/ /pubmed/27181103 http://dx.doi.org/10.1038/srep25918 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Chang, Boyang Yang, Hang Jiao, Yuan Wang, Kefeng Liu, Zhonghua Wu, Peihong Li, Su Wang, Anxun
spellingShingle	Chang, Boyang Yang, Hang Jiao, Yuan Wang, Kefeng Liu, Zhonghua Wu, Peihong Li, Su Wang, Anxun SOD2 deregulation enhances migration, invasion and has poor prognosis in salivary adenoid cystic carcinoma
author_facet	Chang, Boyang Yang, Hang Jiao, Yuan Wang, Kefeng Liu, Zhonghua Wu, Peihong Li, Su Wang, Anxun
author_sort	Chang, Boyang
title	SOD2 deregulation enhances migration, invasion and has poor prognosis in salivary adenoid cystic carcinoma
title_short	SOD2 deregulation enhances migration, invasion and has poor prognosis in salivary adenoid cystic carcinoma
title_full	SOD2 deregulation enhances migration, invasion and has poor prognosis in salivary adenoid cystic carcinoma
title_fullStr	SOD2 deregulation enhances migration, invasion and has poor prognosis in salivary adenoid cystic carcinoma
title_full_unstemmed	SOD2 deregulation enhances migration, invasion and has poor prognosis in salivary adenoid cystic carcinoma
title_sort	sod2 deregulation enhances migration, invasion and has poor prognosis in salivary adenoid cystic carcinoma
description	This study aimed to investigate the role of SOD2 in the progression and metastasis of salivary adenoid cystic carcinoma (SACC). We analyzed the expression of SOD2 in 50 SACC patients. Then, the effects and mechanism of SOD2 on cell metastasis in a pair of different metastatic potential cell lines was investigated. SOD2 was deregulated in patients with SACC. Up-regulation of SOD2 was associated with distant metastasis and reduced overall survival and disease free - survival. Compared to SACC-83 cells (lower metastasis ability), SACC-LM cells (higher metastasis ability) had higher SOD2 activity and intracellular H2O2 concentrations, and protein levels of pERK1/2 and Slug, but had similar catalase protein level and activity. In SACC-LM, reducing the expression of SOD2 by SiRNA inhibited the metastasis ability and reduced the SOD2 activities, intracellular H2O2 concentrations, and protein levels of pERK1/2 and Slug. These effects were revised in SACC-83 after SOD2 overexpression. Moreover, in SACC-83, treated with H2O2, the metastasis was enhanced accompanied by increased protein levels of pERK1/2 and Slug. We confirmed that SOD2 play an important role in the development and prognosis of SACC and SOD2-dependent production of H2O2 contributes to metastasis of SACC through the ERK-Slug signaling pathway.
publisher	Nature Publishing Group
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4867643/
_version_	1613579512277106688

SOD2 deregulation enhances migration, invasion and has poor prognosis in salivary adenoid cystic carcinoma

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