Efficient Photocatalytic Disinfection of Escherichia coli O157:H7 using C70-TiO2 Hybrid under Visible Light Irradiation

Efficient photocatalytic disinfection of Escherichia coli O157:H7 was achieved by using a C70 modified TiO2 (C70-TiO2) hybrid as a photocatalyst under visible light (λ > 420 nm) irradiation. Disinfection experiments showed that 73% of E. coli O157:H7 died within 2 h with a disinfection rate const...

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Main Authors:	Ouyang, Kai, Dai, Ke, Walker, Sharon L., Huang, Qiaoyun, Yin, Xixiang, Cai, Peng
Format:	Online
Language:	English
Published:	Nature Publishing Group 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4861983/

id	pubmed-4861983
recordtype	oai_dc
spelling	pubmed-48619832016-05-23 Efficient Photocatalytic Disinfection of Escherichia coli O157:H7 using C70-TiO2 Hybrid under Visible Light Irradiation Ouyang, Kai Dai, Ke Walker, Sharon L. Huang, Qiaoyun Yin, Xixiang Cai, Peng Article Efficient photocatalytic disinfection of Escherichia coli O157:H7 was achieved by using a C70 modified TiO2 (C70-TiO2) hybrid as a photocatalyst under visible light (λ > 420 nm) irradiation. Disinfection experiments showed that 73% of E. coli O157:H7 died within 2 h with a disinfection rate constant of k = 0.01 min−1, which is three times that measured for TiO2. The mechanism of cell death was investigated by using several scavengers combined with a partition system. The results revealed that diffusing hydroxyl radicals play an important role in the photocatalytically initiated bacterial death, and direct contact between C70-TiO2 hybrid and bacteria is not indispensable in the photocatalytic disinfection process. Extracellular polymeric substances (EPS) of bacteria have little effect on the disinfection efficiency. Analyses of the inhibitory effect of C70-TiO2 thin films on E. coli O157:H7 showed a decrease of the bacterial concentration from 3 × 108 to 38 cfu mL−1 in the solution with C70-TiO2 thin film in the first 2 h of irradiation and a complete inhibition of the growth of E. coli O157:H7 in the later 24 h irradiation. Nature Publishing Group 2016-05-10 /pmc/articles/PMC4861983/ /pubmed/27161821 http://dx.doi.org/10.1038/srep25702 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Ouyang, Kai Dai, Ke Walker, Sharon L. Huang, Qiaoyun Yin, Xixiang Cai, Peng
spellingShingle	Ouyang, Kai Dai, Ke Walker, Sharon L. Huang, Qiaoyun Yin, Xixiang Cai, Peng Efficient Photocatalytic Disinfection of Escherichia coli O157:H7 using C70-TiO2 Hybrid under Visible Light Irradiation
author_facet	Ouyang, Kai Dai, Ke Walker, Sharon L. Huang, Qiaoyun Yin, Xixiang Cai, Peng
author_sort	Ouyang, Kai
title	Efficient Photocatalytic Disinfection of Escherichia coli O157:H7 using C70-TiO2 Hybrid under Visible Light Irradiation
title_short	Efficient Photocatalytic Disinfection of Escherichia coli O157:H7 using C70-TiO2 Hybrid under Visible Light Irradiation
title_full	Efficient Photocatalytic Disinfection of Escherichia coli O157:H7 using C70-TiO2 Hybrid under Visible Light Irradiation
title_fullStr	Efficient Photocatalytic Disinfection of Escherichia coli O157:H7 using C70-TiO2 Hybrid under Visible Light Irradiation
title_full_unstemmed	Efficient Photocatalytic Disinfection of Escherichia coli O157:H7 using C70-TiO2 Hybrid under Visible Light Irradiation
title_sort	efficient photocatalytic disinfection of escherichia coli o157:h7 using c70-tio2 hybrid under visible light irradiation
description	Efficient photocatalytic disinfection of Escherichia coli O157:H7 was achieved by using a C70 modified TiO2 (C70-TiO2) hybrid as a photocatalyst under visible light (λ > 420 nm) irradiation. Disinfection experiments showed that 73% of E. coli O157:H7 died within 2 h with a disinfection rate constant of k = 0.01 min−1, which is three times that measured for TiO2. The mechanism of cell death was investigated by using several scavengers combined with a partition system. The results revealed that diffusing hydroxyl radicals play an important role in the photocatalytically initiated bacterial death, and direct contact between C70-TiO2 hybrid and bacteria is not indispensable in the photocatalytic disinfection process. Extracellular polymeric substances (EPS) of bacteria have little effect on the disinfection efficiency. Analyses of the inhibitory effect of C70-TiO2 thin films on E. coli O157:H7 showed a decrease of the bacterial concentration from 3 × 108 to 38 cfu mL−1 in the solution with C70-TiO2 thin film in the first 2 h of irradiation and a complete inhibition of the growth of E. coli O157:H7 in the later 24 h irradiation.
publisher	Nature Publishing Group
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4861983/
_version_	1613577445088165888

Efficient Photocatalytic Disinfection of Escherichia coli O157:H7 using C70-TiO2 Hybrid under Visible Light Irradiation

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