Persistence of RNAi-Mediated Knockdown in Drosophila Complicates Mosaic Analysis Yet Enables Highly Sensitive Lineage Tracing
RNA interference (RNAi) has emerged as a powerful way of reducing gene function in Drosophila melanogaster tissues. By expressing synthetic short hairpin RNAs (shRNAs) using the Gal4/UAS system, knockdown is efficiently achieved in specific tissues or in clones of marked cells. Here we show that kno...
| Main Authors: | , , |
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| Format: | Online |
| Language: | English |
| Published: |
Genetics Society of America
2016
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4858766/ |