Metabolic Context of the Competence-Induced Checkpoint for Cell Replication in Streptococcus suis

Natural genetic transformation is a transient, rapidly progressing energy-consuming process characterized by expression of the transformasome and competence-associated regulatory genes. This transient state is tightly controlled to avoid potentially adverse effects of genetic recombination on genome...

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Main Authors:	Zaccaria, Edoardo, Wells, Jerry M., van Baarlen, Peter
Format:	Online
Language:	English
Published:	Public Library of Science 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4858297/

id	pubmed-4858297
recordtype	oai_dc
spelling	pubmed-48582972016-05-13 Metabolic Context of the Competence-Induced Checkpoint for Cell Replication in Streptococcus suis Zaccaria, Edoardo Wells, Jerry M. van Baarlen, Peter Research Article Natural genetic transformation is a transient, rapidly progressing energy-consuming process characterized by expression of the transformasome and competence-associated regulatory genes. This transient state is tightly controlled to avoid potentially adverse effects of genetic recombination on genome integrity during cell division. We investigated the global response of Streptococcus suis to exposure to the SigX competence-inducing peptide (XIP), and thus to the activation of the competence machinery, using time series analysis together with PCA analysis, gene clustering followed by heatmap visualisation, and GO enrichment analysis. We explored the possible regulatory link between metabolism and competence, and predicted the physiological adaptation of S. suis during competence induction, progression and exit using transcriptome analysis. We showed that competence development is associated with a suppression of basal metabolism, which may have consequences for the microbe's resilience to fluctuations in the environment, as competence is costly in terms of use of energy and protein translation. Furthermore our data suggest that several basal metabolic pathways are incompatible with activation of competence in S. suis. This study also showed that targeting specific pathways during the development of competence, might render S. suis more vulnerable toward novel antibiotic therapies. Public Library of Science 2016-05-05 /pmc/articles/PMC4858297/ /pubmed/27149631 http://dx.doi.org/10.1371/journal.pone.0153571 Text en © 2016 Zaccaria et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Zaccaria, Edoardo Wells, Jerry M. van Baarlen, Peter
spellingShingle	Zaccaria, Edoardo Wells, Jerry M. van Baarlen, Peter Metabolic Context of the Competence-Induced Checkpoint for Cell Replication in Streptococcus suis
author_facet	Zaccaria, Edoardo Wells, Jerry M. van Baarlen, Peter
author_sort	Zaccaria, Edoardo
title	Metabolic Context of the Competence-Induced Checkpoint for Cell Replication in Streptococcus suis
title_short	Metabolic Context of the Competence-Induced Checkpoint for Cell Replication in Streptococcus suis
title_full	Metabolic Context of the Competence-Induced Checkpoint for Cell Replication in Streptococcus suis
title_fullStr	Metabolic Context of the Competence-Induced Checkpoint for Cell Replication in Streptococcus suis
title_full_unstemmed	Metabolic Context of the Competence-Induced Checkpoint for Cell Replication in Streptococcus suis
title_sort	metabolic context of the competence-induced checkpoint for cell replication in streptococcus suis
description	Natural genetic transformation is a transient, rapidly progressing energy-consuming process characterized by expression of the transformasome and competence-associated regulatory genes. This transient state is tightly controlled to avoid potentially adverse effects of genetic recombination on genome integrity during cell division. We investigated the global response of Streptococcus suis to exposure to the SigX competence-inducing peptide (XIP), and thus to the activation of the competence machinery, using time series analysis together with PCA analysis, gene clustering followed by heatmap visualisation, and GO enrichment analysis. We explored the possible regulatory link between metabolism and competence, and predicted the physiological adaptation of S. suis during competence induction, progression and exit using transcriptome analysis. We showed that competence development is associated with a suppression of basal metabolism, which may have consequences for the microbe's resilience to fluctuations in the environment, as competence is costly in terms of use of energy and protein translation. Furthermore our data suggest that several basal metabolic pathways are incompatible with activation of competence in S. suis. This study also showed that targeting specific pathways during the development of competence, might render S. suis more vulnerable toward novel antibiotic therapies.
publisher	Public Library of Science
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4858297/
_version_	1613576114103386112

Metabolic Context of the Competence-Induced Checkpoint for Cell Replication in Streptococcus suis

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