Pro-inflammatory effects of a litchi protein extract in murine RAW264.7 macrophages

It has been observed that the consumption of litchi often causes symptoms characterized by itching or sore throat, gum swelling, oral cavity ulcers and even fever and inflammation, which significantly impair the quality of life of a large population. Using the RAW264.7 cell line, a step-by-step stra...

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Main Authors:	Wang, Xiaoli, Hu, Xiaorong, Yan, Huiqing, Ma, Zhaocheng, Deng, Xiuxin
Format:	Online
Language:	English
Published:	Nature Publishing Group 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4855250/

id	pubmed-4855250
recordtype	oai_dc
spelling	pubmed-48552502016-05-18 Pro-inflammatory effects of a litchi protein extract in murine RAW264.7 macrophages Wang, Xiaoli Hu, Xiaorong Yan, Huiqing Ma, Zhaocheng Deng, Xiuxin Article It has been observed that the consumption of litchi often causes symptoms characterized by itching or sore throat, gum swelling, oral cavity ulcers and even fever and inflammation, which significantly impair the quality of life of a large population. Using the RAW264.7 cell line, a step-by-step strategy was used to screen for the components in litchi fruits that elicited adverse reactions. The adverse reaction fractions were identified by mass spectrometry and analyzed using the SMART program, and a sequence alignment of the homologous proteins was performed. MTT tests were used to determine the cytotoxicity of a litchi protein extract in RAW264.7 macrophages, and real-time PCR was applied to analyze the expression of inflammatory genes in the RAW264.7 cells treated with lipopolysaccharide or the litchi protein extract. The results showed that the litchi water-soluble protein extract could increase the production of the pro-inflammatory mediators IL-1β, iNOS and COX-2, and the anti-inflammatory mediator HO-1 in the RAW264.7 cell line. The 14-3-3-like proteins GF14 lambda, GF14 omega and GF14 upsilon were likely the candidate proteins that caused the adverse effects. Nature Publishing Group 2016-05-04 /pmc/articles/PMC4855250/ /pubmed/27195125 http://dx.doi.org/10.1038/hortres.2016.17 Text en Copyright © 2016 Nanjing Agricultural University http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Wang, Xiaoli Hu, Xiaorong Yan, Huiqing Ma, Zhaocheng Deng, Xiuxin
spellingShingle	Wang, Xiaoli Hu, Xiaorong Yan, Huiqing Ma, Zhaocheng Deng, Xiuxin Pro-inflammatory effects of a litchi protein extract in murine RAW264.7 macrophages
author_facet	Wang, Xiaoli Hu, Xiaorong Yan, Huiqing Ma, Zhaocheng Deng, Xiuxin
author_sort	Wang, Xiaoli
title	Pro-inflammatory effects of a litchi protein extract in murine RAW264.7 macrophages
title_short	Pro-inflammatory effects of a litchi protein extract in murine RAW264.7 macrophages
title_full	Pro-inflammatory effects of a litchi protein extract in murine RAW264.7 macrophages
title_fullStr	Pro-inflammatory effects of a litchi protein extract in murine RAW264.7 macrophages
title_full_unstemmed	Pro-inflammatory effects of a litchi protein extract in murine RAW264.7 macrophages
title_sort	pro-inflammatory effects of a litchi protein extract in murine raw264.7 macrophages
description	It has been observed that the consumption of litchi often causes symptoms characterized by itching or sore throat, gum swelling, oral cavity ulcers and even fever and inflammation, which significantly impair the quality of life of a large population. Using the RAW264.7 cell line, a step-by-step strategy was used to screen for the components in litchi fruits that elicited adverse reactions. The adverse reaction fractions were identified by mass spectrometry and analyzed using the SMART program, and a sequence alignment of the homologous proteins was performed. MTT tests were used to determine the cytotoxicity of a litchi protein extract in RAW264.7 macrophages, and real-time PCR was applied to analyze the expression of inflammatory genes in the RAW264.7 cells treated with lipopolysaccharide or the litchi protein extract. The results showed that the litchi water-soluble protein extract could increase the production of the pro-inflammatory mediators IL-1β, iNOS and COX-2, and the anti-inflammatory mediator HO-1 in the RAW264.7 cell line. The 14-3-3-like proteins GF14 lambda, GF14 omega and GF14 upsilon were likely the candidate proteins that caused the adverse effects.
publisher	Nature Publishing Group
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4855250/
_version_	1613574892916047872

Pro-inflammatory effects of a litchi protein extract in murine RAW264.7 macrophages

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