Microfluidics for simultaneous quantification of platelet adhesion and blood viscosity

Platelet functions, including adhesion, activation, and aggregation have an influence on thrombosis and the progression of atherosclerosis. In the present study, a new microfluidic-based method is proposed to estimate platelet adhesion and blood viscosity simultaneously. Blood sample flows into an H...

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Main Authors:	Yeom, Eunseop, Park, Jun Hong, Kang, Yang Jun, Lee, Sang Joon
Format:	Online
Language:	English
Published:	Nature Publishing Group 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4846989/

id	pubmed-4846989
recordtype	oai_dc
spelling	pubmed-48469892016-05-04 Microfluidics for simultaneous quantification of platelet adhesion and blood viscosity Yeom, Eunseop Park, Jun Hong Kang, Yang Jun Lee, Sang Joon Article Platelet functions, including adhesion, activation, and aggregation have an influence on thrombosis and the progression of atherosclerosis. In the present study, a new microfluidic-based method is proposed to estimate platelet adhesion and blood viscosity simultaneously. Blood sample flows into an H-shaped microfluidic device with a peristaltic pump. Since platelet aggregation may be initiated by the compression of rotors inside the peristaltic pump, platelet aggregates may adhere to the H-shaped channel. Through correlation mapping, which visualizes decorrelation of the streaming blood flow, the area of adhered platelets (APlatelet) can be estimated without labeling platelets. The platelet function is estimated by determining the representative index IA·T based on APlatelet and contact time. Blood viscosity is measured by monitoring the flow conditions in the one side channel of the H-shaped device. Based on the relation between interfacial width (W) and pressure ratio of sample flows to the reference, blood sample viscosity (μ) can be estimated by measuring W. Biophysical parameters (IA·T, μ) are compared for normal and diabetic rats using an ex vivo extracorporeal model. This microfluidic-based method can be used for evaluating variations in the platelet adhesion and blood viscosity of animal models with cardiovascular diseases under ex vivo conditions. Nature Publishing Group 2016-04-27 /pmc/articles/PMC4846989/ /pubmed/27118101 http://dx.doi.org/10.1038/srep24994 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Yeom, Eunseop Park, Jun Hong Kang, Yang Jun Lee, Sang Joon
spellingShingle	Yeom, Eunseop Park, Jun Hong Kang, Yang Jun Lee, Sang Joon Microfluidics for simultaneous quantification of platelet adhesion and blood viscosity
author_facet	Yeom, Eunseop Park, Jun Hong Kang, Yang Jun Lee, Sang Joon
author_sort	Yeom, Eunseop
title	Microfluidics for simultaneous quantification of platelet adhesion and blood viscosity
title_short	Microfluidics for simultaneous quantification of platelet adhesion and blood viscosity
title_full	Microfluidics for simultaneous quantification of platelet adhesion and blood viscosity
title_fullStr	Microfluidics for simultaneous quantification of platelet adhesion and blood viscosity
title_full_unstemmed	Microfluidics for simultaneous quantification of platelet adhesion and blood viscosity
title_sort	microfluidics for simultaneous quantification of platelet adhesion and blood viscosity
description	Platelet functions, including adhesion, activation, and aggregation have an influence on thrombosis and the progression of atherosclerosis. In the present study, a new microfluidic-based method is proposed to estimate platelet adhesion and blood viscosity simultaneously. Blood sample flows into an H-shaped microfluidic device with a peristaltic pump. Since platelet aggregation may be initiated by the compression of rotors inside the peristaltic pump, platelet aggregates may adhere to the H-shaped channel. Through correlation mapping, which visualizes decorrelation of the streaming blood flow, the area of adhered platelets (APlatelet) can be estimated without labeling platelets. The platelet function is estimated by determining the representative index IA·T based on APlatelet and contact time. Blood viscosity is measured by monitoring the flow conditions in the one side channel of the H-shaped device. Based on the relation between interfacial width (W) and pressure ratio of sample flows to the reference, blood sample viscosity (μ) can be estimated by measuring W. Biophysical parameters (IA·T, μ) are compared for normal and diabetic rats using an ex vivo extracorporeal model. This microfluidic-based method can be used for evaluating variations in the platelet adhesion and blood viscosity of animal models with cardiovascular diseases under ex vivo conditions.
publisher	Nature Publishing Group
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4846989/
_version_	1613571624849637376

Microfluidics for simultaneous quantification of platelet adhesion and blood viscosity

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