Large-scale identification of small noncoding RNA with strand-specific deep sequencing and characterization of a novel virulence-related sRNA in Brucella melitensis
Brucella is the causative agent of brucellosis, a worldwide epidemic zoonosis. Small noncoding RNAs (sRNAs) are important modulators of gene expression and involved in pathogenesis and stress adaptation of Brucella. In this study, using a strand-specific RNA deep-sequencing approach, we identified a...
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| Format: | Online |
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Nature Publishing Group
2016
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4845025/ |
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pubmed-48450252016-04-29 Large-scale identification of small noncoding RNA with strand-specific deep sequencing and characterization of a novel virulence-related sRNA in Brucella melitensis Zhong, Zhijun Xu, Xiaoyang Li, Xinran Liu, Shiwei Lei, Shuangshuang Yang, Mingjuan Yu, Jiuxuan Yuan, Jiuyun Ke, Yuehua Du, Xinying Wang, Zhoujia Ren, Zhihua Peng, Guangneng Wang, Yufei Chen, Zeliang Article Brucella is the causative agent of brucellosis, a worldwide epidemic zoonosis. Small noncoding RNAs (sRNAs) are important modulators of gene expression and involved in pathogenesis and stress adaptation of Brucella. In this study, using a strand-specific RNA deep-sequencing approach, we identified a global set of sRNAs expressed by B. melitensis 16M. In total, 1321 sRNAs were identified, ranging from 100 to 600 nucleotides. These sRNAs differ in their expression levels and strand and chromosomal distributions. The role of BSR0441, one of these sRNAs, in the virulence of B. melitensis 16M was further characterized. BSR0441 was highly induced during the infection of macrophages and mice. The deletion mutant of BSR0441 showed significantly reduced spleen colonization in the middle and late phases of infection. The expression of the BSR0441 target mRNA genes was also altered in the BSR0441 mutant strain during macrophage and mice infection, which is consistent with its reduced intracellular survival capacity. In summary, Brucella encodes a large number of sRNAs, which may be involved in the stress adaptation and virulence of Brucella. Further investigation of these regulators will extend our understanding of the Brucella pathogenesis mechanism and the interactions between Brucella and its hosts. Nature Publishing Group 2016-04-26 /pmc/articles/PMC4845025/ /pubmed/27112796 http://dx.doi.org/10.1038/srep25123 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Zhong, Zhijun Xu, Xiaoyang Li, Xinran Liu, Shiwei Lei, Shuangshuang Yang, Mingjuan Yu, Jiuxuan Yuan, Jiuyun Ke, Yuehua Du, Xinying Wang, Zhoujia Ren, Zhihua Peng, Guangneng Wang, Yufei Chen, Zeliang |
| spellingShingle |
Zhong, Zhijun Xu, Xiaoyang Li, Xinran Liu, Shiwei Lei, Shuangshuang Yang, Mingjuan Yu, Jiuxuan Yuan, Jiuyun Ke, Yuehua Du, Xinying Wang, Zhoujia Ren, Zhihua Peng, Guangneng Wang, Yufei Chen, Zeliang Large-scale identification of small noncoding RNA with strand-specific deep sequencing and characterization of a novel virulence-related sRNA in Brucella melitensis |
| author_facet |
Zhong, Zhijun Xu, Xiaoyang Li, Xinran Liu, Shiwei Lei, Shuangshuang Yang, Mingjuan Yu, Jiuxuan Yuan, Jiuyun Ke, Yuehua Du, Xinying Wang, Zhoujia Ren, Zhihua Peng, Guangneng Wang, Yufei Chen, Zeliang |
| author_sort |
Zhong, Zhijun |
| title |
Large-scale identification of small noncoding RNA with strand-specific deep sequencing and characterization of a novel virulence-related sRNA in Brucella melitensis |
| title_short |
Large-scale identification of small noncoding RNA with strand-specific deep sequencing and characterization of a novel virulence-related sRNA in Brucella melitensis |
| title_full |
Large-scale identification of small noncoding RNA with strand-specific deep sequencing and characterization of a novel virulence-related sRNA in Brucella melitensis |
| title_fullStr |
Large-scale identification of small noncoding RNA with strand-specific deep sequencing and characterization of a novel virulence-related sRNA in Brucella melitensis |
| title_full_unstemmed |
Large-scale identification of small noncoding RNA with strand-specific deep sequencing and characterization of a novel virulence-related sRNA in Brucella melitensis |
| title_sort |
large-scale identification of small noncoding rna with strand-specific deep sequencing and characterization of a novel virulence-related srna in brucella melitensis |
| description |
Brucella is the causative agent of brucellosis, a worldwide epidemic zoonosis. Small noncoding RNAs (sRNAs) are important modulators of gene expression and involved in pathogenesis and stress adaptation of Brucella. In this study, using a strand-specific RNA deep-sequencing approach, we identified a global set of sRNAs expressed by B. melitensis 16M. In total, 1321 sRNAs were identified, ranging from 100 to 600 nucleotides. These sRNAs differ in their expression levels and strand and chromosomal distributions. The role of BSR0441, one of these sRNAs, in the virulence of B. melitensis 16M was further characterized. BSR0441 was highly induced during the infection of macrophages and mice. The deletion mutant of BSR0441 showed significantly reduced spleen colonization in the middle and late phases of infection. The expression of the BSR0441 target mRNA genes was also altered in the BSR0441 mutant strain during macrophage and mice infection, which is consistent with its reduced intracellular survival capacity. In summary, Brucella encodes a large number of sRNAs, which may be involved in the stress adaptation and virulence of Brucella. Further investigation of these regulators will extend our understanding of the Brucella pathogenesis mechanism and the interactions between Brucella and its hosts. |
| publisher |
Nature Publishing Group |
| publishDate |
2016 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4845025/ |
| _version_ |
1613570944336396288 |