Oxidative Stress and Metabolic Perturbations in Wooden Breast Disorder in Chickens
This study was conducted to characterize metabolic features of the breast muscle (pectoralis major) in chickens affected with the Wooden Breast myopathy. Live birds from two purebred chicken lines and one crossbred commercial broiler population were clinically examined by manual palpation of the bre...
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| Format: | Online |
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Public Library of Science
2016
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4838225/ |
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pubmed-48382252016-04-29 Oxidative Stress and Metabolic Perturbations in Wooden Breast Disorder in Chickens Abasht, Behnam Mutryn, Marie F. Michalek, Ryan D. Lee, William R. Research Article This study was conducted to characterize metabolic features of the breast muscle (pectoralis major) in chickens affected with the Wooden Breast myopathy. Live birds from two purebred chicken lines and one crossbred commercial broiler population were clinically examined by manual palpation of the breast muscle (pectoralis major) at 47–48 days of age. Metabolite abundance was determined by gas chromatography/mass spectrometry (GC/MS) and liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) using breast muscle tissue samples from 16 affected and 16 unaffected chickens. Muscle glycogen content was also quantified in breast muscle tissue samples from affected and unaffected chickens. In total, levels of 140 biochemicals were significantly different (FDR < 0.1 and fold-change A/U > 1.3 or < 0.77) between affected and unaffected chickens. Glycogen content measurements were considerably lower (1.7-fold) in samples taken from Wooden Breast affected birds when compared with samples from unaffected birds. Affected tissues exhibited biomarkers related to increased oxidative stress, elevated protein levels, muscle degradation, and altered glucose utilization. Affected muscle also showed elevated levels of hypoxanthine, xanthine, and urate molecules, the generation of which can contribute to altered redox homeostasis. In conclusion, our findings show that Wooden Breast affected tissues possess a unique metabolic signature. This unique profile may identify candidate biomarkers for diagnostic utilization and provide mechanistic insight into altered biochemical processes contributing to tissue hardening associated with the Wooden Breast myopathy in commercial chickens. Public Library of Science 2016-04-20 /pmc/articles/PMC4838225/ /pubmed/27097013 http://dx.doi.org/10.1371/journal.pone.0153750 Text en © 2016 Abasht et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Abasht, Behnam Mutryn, Marie F. Michalek, Ryan D. Lee, William R. |
| spellingShingle |
Abasht, Behnam Mutryn, Marie F. Michalek, Ryan D. Lee, William R. Oxidative Stress and Metabolic Perturbations in Wooden Breast Disorder in Chickens |
| author_facet |
Abasht, Behnam Mutryn, Marie F. Michalek, Ryan D. Lee, William R. |
| author_sort |
Abasht, Behnam |
| title |
Oxidative Stress and Metabolic Perturbations in Wooden Breast Disorder in Chickens |
| title_short |
Oxidative Stress and Metabolic Perturbations in Wooden Breast Disorder in Chickens |
| title_full |
Oxidative Stress and Metabolic Perturbations in Wooden Breast Disorder in Chickens |
| title_fullStr |
Oxidative Stress and Metabolic Perturbations in Wooden Breast Disorder in Chickens |
| title_full_unstemmed |
Oxidative Stress and Metabolic Perturbations in Wooden Breast Disorder in Chickens |
| title_sort |
oxidative stress and metabolic perturbations in wooden breast disorder in chickens |
| description |
This study was conducted to characterize metabolic features of the breast muscle (pectoralis major) in chickens affected with the Wooden Breast myopathy. Live birds from two purebred chicken lines and one crossbred commercial broiler population were clinically examined by manual palpation of the breast muscle (pectoralis major) at 47–48 days of age. Metabolite abundance was determined by gas chromatography/mass spectrometry (GC/MS) and liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) using breast muscle tissue samples from 16 affected and 16 unaffected chickens. Muscle glycogen content was also quantified in breast muscle tissue samples from affected and unaffected chickens. In total, levels of 140 biochemicals were significantly different (FDR < 0.1 and fold-change A/U > 1.3 or < 0.77) between affected and unaffected chickens. Glycogen content measurements were considerably lower (1.7-fold) in samples taken from Wooden Breast affected birds when compared with samples from unaffected birds. Affected tissues exhibited biomarkers related to increased oxidative stress, elevated protein levels, muscle degradation, and altered glucose utilization. Affected muscle also showed elevated levels of hypoxanthine, xanthine, and urate molecules, the generation of which can contribute to altered redox homeostasis. In conclusion, our findings show that Wooden Breast affected tissues possess a unique metabolic signature. This unique profile may identify candidate biomarkers for diagnostic utilization and provide mechanistic insight into altered biochemical processes contributing to tissue hardening associated with the Wooden Breast myopathy in commercial chickens. |
| publisher |
Public Library of Science |
| publishDate |
2016 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4838225/ |
| _version_ |
1613568502135783424 |