Standard melanoma-associated markers do not identify the MM127 metastatic melanoma cell line
Reliable identification of different melanoma cell lines is important for many aspects of melanoma research. Common markers used to identify melanoma cell lines include: S100; HMB-45; and Melan-A. We explore the expression of these three markers in four different melanoma cell lines: WM35; WM793; SK...
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| Format: | Online |
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Nature Publishing Group
2016
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4834532/ |
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pubmed-48345322016-04-27 Standard melanoma-associated markers do not identify the MM127 metastatic melanoma cell line Haridas, Parvathi McGovern, Jacqui A. Kashyap, Abhishek S. McElwain, D. L. Sean Simpson, Matthew J. Article Reliable identification of different melanoma cell lines is important for many aspects of melanoma research. Common markers used to identify melanoma cell lines include: S100; HMB-45; and Melan-A. We explore the expression of these three markers in four different melanoma cell lines: WM35; WM793; SK-MEL-28; and MM127. The expression of these markers is examined at both the mRNA and protein level. Our results show that the metastatic cell line, MM127, cannot be detected using any of the commonly used melanoma-associated markers. This implies that it would be very difficult to identify this particular cell line in a heterogeneous sample, and as a result this cell line should be used with care. Nature Publishing Group 2016-04-18 /pmc/articles/PMC4834532/ /pubmed/27087056 http://dx.doi.org/10.1038/srep24569 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Haridas, Parvathi McGovern, Jacqui A. Kashyap, Abhishek S. McElwain, D. L. Sean Simpson, Matthew J. |
| spellingShingle |
Haridas, Parvathi McGovern, Jacqui A. Kashyap, Abhishek S. McElwain, D. L. Sean Simpson, Matthew J. Standard melanoma-associated markers do not identify the MM127 metastatic melanoma cell line |
| author_facet |
Haridas, Parvathi McGovern, Jacqui A. Kashyap, Abhishek S. McElwain, D. L. Sean Simpson, Matthew J. |
| author_sort |
Haridas, Parvathi |
| title |
Standard melanoma-associated markers do not identify the MM127 metastatic melanoma cell line |
| title_short |
Standard melanoma-associated markers do not identify the MM127 metastatic melanoma cell line |
| title_full |
Standard melanoma-associated markers do not identify the MM127 metastatic melanoma cell line |
| title_fullStr |
Standard melanoma-associated markers do not identify the MM127 metastatic melanoma cell line |
| title_full_unstemmed |
Standard melanoma-associated markers do not identify the MM127 metastatic melanoma cell line |
| title_sort |
standard melanoma-associated markers do not identify the mm127 metastatic melanoma cell line |
| description |
Reliable identification of different melanoma cell lines is important for many aspects of melanoma research. Common markers used to identify melanoma cell lines include: S100; HMB-45; and Melan-A. We explore the expression of these three markers in four different melanoma cell lines: WM35; WM793; SK-MEL-28; and MM127. The expression of these markers is examined at both the mRNA and protein level. Our results show that the metastatic cell line, MM127, cannot be detected using any of the commonly used melanoma-associated markers. This implies that it would be very difficult to identify this particular cell line in a heterogeneous sample, and as a result this cell line should be used with care. |
| publisher |
Nature Publishing Group |
| publishDate |
2016 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4834532/ |
| _version_ |
1613567326480760832 |