A Carotenoid-Deficient Mutant in Pantoea sp. YR343, a Bacteria Isolated from the Rhizosphere of Populus deltoides, Is Defective in Root Colonization

The complex interactions between plants and their microbiome can have a profound effect on the health and productivity of the plant host. A better understanding of the microbial mechanisms that promote plant health and stress tolerance will enable strategies for improving the productivity of economi...

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Main Authors:	Bible, Amber N., Fletcher, Sarah J., Pelletier, Dale A., Schadt, Christopher W., Jawdy, Sara S., Weston, David J., Engle, Nancy L., Tschaplinski, Timothy, Masyuko, Rachel, Polisetti, Sneha, Bohn, Paul W., Coutinho, Teresa A., Doktycz, Mitchel J., Morrell-Falvey, Jennifer L.
Format:	Online
Language:	English
Published:	Frontiers Media S.A. 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4834302/

id	pubmed-4834302
recordtype	oai_dc
spelling	pubmed-48343022016-05-04 A Carotenoid-Deficient Mutant in Pantoea sp. YR343, a Bacteria Isolated from the Rhizosphere of Populus deltoides, Is Defective in Root Colonization Bible, Amber N. Fletcher, Sarah J. Pelletier, Dale A. Schadt, Christopher W. Jawdy, Sara S. Weston, David J. Engle, Nancy L. Tschaplinski, Timothy Masyuko, Rachel Polisetti, Sneha Bohn, Paul W. Coutinho, Teresa A. Doktycz, Mitchel J. Morrell-Falvey, Jennifer L. Microbiology The complex interactions between plants and their microbiome can have a profound effect on the health and productivity of the plant host. A better understanding of the microbial mechanisms that promote plant health and stress tolerance will enable strategies for improving the productivity of economically important plants. Pantoea sp. YR343 is a motile, rod-shaped bacterium isolated from the roots of Populus deltoides that possesses the ability to solubilize phosphate and produce the phytohormone indole-3-acetic acid (IAA). Pantoea sp. YR343 readily colonizes plant roots and does not appear to be pathogenic when applied to the leaves or roots of selected plant hosts. To better understand the molecular mechanisms involved in plant association and rhizosphere survival by Pantoea sp. YR343, we constructed a mutant in which the crtB gene encoding phytoene synthase was deleted. Phytoene synthase is responsible for converting geranylgeranyl pyrophosphate to phytoene, an important precursor to the production of carotenoids. As predicted, the ΔcrtB mutant is defective in carotenoid production, and shows increased sensitivity to oxidative stress. Moreover, we find that the ΔcrtB mutant is impaired in biofilm formation and production of IAA. Finally we demonstrate that the ΔcrtB mutant shows reduced colonization of plant roots. Taken together, these data suggest that carotenoids are important for plant association and/or rhizosphere survival in Pantoea sp. YR343. Frontiers Media S.A. 2016-04-18 /pmc/articles/PMC4834302/ /pubmed/27148182 http://dx.doi.org/10.3389/fmicb.2016.00491 Text en Copyright © 2016 Bible, Fletcher, Pelletier, Schadt, Jawdy, Weston, Engle, Tschaplinski, Masyuko, Polisetti, Bohn, Coutinho, Doktycz and Morrell-Falvey. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Bible, Amber N. Fletcher, Sarah J. Pelletier, Dale A. Schadt, Christopher W. Jawdy, Sara S. Weston, David J. Engle, Nancy L. Tschaplinski, Timothy Masyuko, Rachel Polisetti, Sneha Bohn, Paul W. Coutinho, Teresa A. Doktycz, Mitchel J. Morrell-Falvey, Jennifer L.
spellingShingle	Bible, Amber N. Fletcher, Sarah J. Pelletier, Dale A. Schadt, Christopher W. Jawdy, Sara S. Weston, David J. Engle, Nancy L. Tschaplinski, Timothy Masyuko, Rachel Polisetti, Sneha Bohn, Paul W. Coutinho, Teresa A. Doktycz, Mitchel J. Morrell-Falvey, Jennifer L. A Carotenoid-Deficient Mutant in Pantoea sp. YR343, a Bacteria Isolated from the Rhizosphere of Populus deltoides, Is Defective in Root Colonization
author_facet	Bible, Amber N. Fletcher, Sarah J. Pelletier, Dale A. Schadt, Christopher W. Jawdy, Sara S. Weston, David J. Engle, Nancy L. Tschaplinski, Timothy Masyuko, Rachel Polisetti, Sneha Bohn, Paul W. Coutinho, Teresa A. Doktycz, Mitchel J. Morrell-Falvey, Jennifer L.
author_sort	Bible, Amber N.
title	A Carotenoid-Deficient Mutant in Pantoea sp. YR343, a Bacteria Isolated from the Rhizosphere of Populus deltoides, Is Defective in Root Colonization
title_short	A Carotenoid-Deficient Mutant in Pantoea sp. YR343, a Bacteria Isolated from the Rhizosphere of Populus deltoides, Is Defective in Root Colonization
title_full	A Carotenoid-Deficient Mutant in Pantoea sp. YR343, a Bacteria Isolated from the Rhizosphere of Populus deltoides, Is Defective in Root Colonization
title_fullStr	A Carotenoid-Deficient Mutant in Pantoea sp. YR343, a Bacteria Isolated from the Rhizosphere of Populus deltoides, Is Defective in Root Colonization
title_full_unstemmed	A Carotenoid-Deficient Mutant in Pantoea sp. YR343, a Bacteria Isolated from the Rhizosphere of Populus deltoides, Is Defective in Root Colonization
title_sort	carotenoid-deficient mutant in pantoea sp. yr343, a bacteria isolated from the rhizosphere of populus deltoides, is defective in root colonization
description	The complex interactions between plants and their microbiome can have a profound effect on the health and productivity of the plant host. A better understanding of the microbial mechanisms that promote plant health and stress tolerance will enable strategies for improving the productivity of economically important plants. Pantoea sp. YR343 is a motile, rod-shaped bacterium isolated from the roots of Populus deltoides that possesses the ability to solubilize phosphate and produce the phytohormone indole-3-acetic acid (IAA). Pantoea sp. YR343 readily colonizes plant roots and does not appear to be pathogenic when applied to the leaves or roots of selected plant hosts. To better understand the molecular mechanisms involved in plant association and rhizosphere survival by Pantoea sp. YR343, we constructed a mutant in which the crtB gene encoding phytoene synthase was deleted. Phytoene synthase is responsible for converting geranylgeranyl pyrophosphate to phytoene, an important precursor to the production of carotenoids. As predicted, the ΔcrtB mutant is defective in carotenoid production, and shows increased sensitivity to oxidative stress. Moreover, we find that the ΔcrtB mutant is impaired in biofilm formation and production of IAA. Finally we demonstrate that the ΔcrtB mutant shows reduced colonization of plant roots. Taken together, these data suggest that carotenoids are important for plant association and/or rhizosphere survival in Pantoea sp. YR343.
publisher	Frontiers Media S.A.
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4834302/
_version_	1613567224538202112

A Carotenoid-Deficient Mutant in Pantoea sp. YR343, a Bacteria Isolated from the Rhizosphere of Populus deltoides, Is Defective in Root Colonization

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