An improved smaller biotin ligase for BioID proximity labeling

A smaller promiscuous biotin ligase for proximity biotinylation called BioID2 enables more-selective targeting of fusion proteins, requires less biotin supplementation, exhibits enhanced labeling of proximate proteins, and demonstrates the use of a flexible linker to modulate the biotin-labeling rad...

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Bibliographic Details
Main Authors: Kim, Dae In, Jensen, Samuel C., Noble, Kyle A., KC, Birendra, Roux, Kenneth H., Motamedchaboki, Khatereh, Roux, Kyle J.
Format: Online
Language:English
Published: The American Society for Cell Biology 2016
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4831873/