Sequencing 16S rRNA gene fragments using the PacBio SMRT DNA sequencing system

Over the past 10 years, microbial ecologists have largely abandoned sequencing 16S rRNA genes by the Sanger sequencing method and have instead adopted highly parallelized sequencing platforms. These new platforms, such as 454 and Illumina’s MiSeq, have allowed researchers to obtain millions of high...

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Bibliographic Details
Main Authors: Schloss, Patrick D., Jenior, Matthew L., Koumpouras, Charles C., Westcott, Sarah L., Highlander, Sarah K.
Format: Online
Language:English
Published: PeerJ Inc. 2016
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4824876/