How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments
We have examined the imprecision in the estimation of PCR efficiency by means of standard curves based on strategic experimental design with large number of technical replicates. In particular, how robust this estimation is in terms of a commonly varying factors: the instrument used, the number of t...
| Main Authors: | , , , , |
|---|---|
| Format: | Online |
| Language: | English |
| Published: |
Elsevier
2015
|
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4822216/ |