Discovery of piRNAs Pathway Associated with Early-Stage Spermatogenesis in Chicken
Piwi-interacting RNAs (piRNAs) play a key role in spermatogenesis. Here, we describe the piRNAs profiling of primordial germ cells (PGCs), spermatogonial stem cells (SSCs), and the spermatogonium (Sp) during early-stage spermatogenesis in chicken. We obtained 31,361,989 reads from PGCs, 31,757,666 r...
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| Format: | Online |
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Public Library of Science
2016
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4821617/ |
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pubmed-4821617 |
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pubmed-48216172016-04-22 Discovery of piRNAs Pathway Associated with Early-Stage Spermatogenesis in Chicken Xu, Lu Qiu, Lingling Chang, Guobin Guo, Qixin Liu, Xiangping Bi, Yulin Zhang, Yu Wang, Hongzhi Li, Zhiteng Guo, Xiaoming Wan, Fang Zhang, Yang Xu, Qi Chen, Guohong Research Article Piwi-interacting RNAs (piRNAs) play a key role in spermatogenesis. Here, we describe the piRNAs profiling of primordial germ cells (PGCs), spermatogonial stem cells (SSCs), and the spermatogonium (Sp) during early-stage spermatogenesis in chicken. We obtained 31,361,989 reads from PGCs, 31,757,666 reads from SSCs, and 46,448,327 reads from Sp cells. The length distribution of piRNAs in the three samples showed peaks at 33 nt. The resulting genes were subsequently annotated against the Gene Ontology (GO) database. Five genes (RPL7A, HSPA8, Pum1, CPXM2, and PRKCA) were found to be involved in cellular processes. Interactive pathway analysis (IPA) further revealed three important pathways in early-stage spermatogenesis including the FGF, Wnt, and EGF receptor signaling pathways. The gene Pum1 was found to promote germline stem cell proliferation, but it also plays a role in spermatogenesis. In conclusion, we revealed characteristics of piRNAs during early spermatogonial development in chicken and provided the basis for future research. Public Library of Science 2016-04-05 /pmc/articles/PMC4821617/ /pubmed/27045806 http://dx.doi.org/10.1371/journal.pone.0151780 Text en © 2016 Xu et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Xu, Lu Qiu, Lingling Chang, Guobin Guo, Qixin Liu, Xiangping Bi, Yulin Zhang, Yu Wang, Hongzhi Li, Zhiteng Guo, Xiaoming Wan, Fang Zhang, Yang Xu, Qi Chen, Guohong |
| spellingShingle |
Xu, Lu Qiu, Lingling Chang, Guobin Guo, Qixin Liu, Xiangping Bi, Yulin Zhang, Yu Wang, Hongzhi Li, Zhiteng Guo, Xiaoming Wan, Fang Zhang, Yang Xu, Qi Chen, Guohong Discovery of piRNAs Pathway Associated with Early-Stage Spermatogenesis in Chicken |
| author_facet |
Xu, Lu Qiu, Lingling Chang, Guobin Guo, Qixin Liu, Xiangping Bi, Yulin Zhang, Yu Wang, Hongzhi Li, Zhiteng Guo, Xiaoming Wan, Fang Zhang, Yang Xu, Qi Chen, Guohong |
| author_sort |
Xu, Lu |
| title |
Discovery of piRNAs Pathway Associated with Early-Stage Spermatogenesis in Chicken |
| title_short |
Discovery of piRNAs Pathway Associated with Early-Stage Spermatogenesis in Chicken |
| title_full |
Discovery of piRNAs Pathway Associated with Early-Stage Spermatogenesis in Chicken |
| title_fullStr |
Discovery of piRNAs Pathway Associated with Early-Stage Spermatogenesis in Chicken |
| title_full_unstemmed |
Discovery of piRNAs Pathway Associated with Early-Stage Spermatogenesis in Chicken |
| title_sort |
discovery of pirnas pathway associated with early-stage spermatogenesis in chicken |
| description |
Piwi-interacting RNAs (piRNAs) play a key role in spermatogenesis. Here, we describe the piRNAs profiling of primordial germ cells (PGCs), spermatogonial stem cells (SSCs), and the spermatogonium (Sp) during early-stage spermatogenesis in chicken. We obtained 31,361,989 reads from PGCs, 31,757,666 reads from SSCs, and 46,448,327 reads from Sp cells. The length distribution of piRNAs in the three samples showed peaks at 33 nt. The resulting genes were subsequently annotated against the Gene Ontology (GO) database. Five genes (RPL7A, HSPA8, Pum1, CPXM2, and PRKCA) were found to be involved in cellular processes. Interactive pathway analysis (IPA) further revealed three important pathways in early-stage spermatogenesis including the FGF, Wnt, and EGF receptor signaling pathways. The gene Pum1 was found to promote germline stem cell proliferation, but it also plays a role in spermatogenesis. In conclusion, we revealed characteristics of piRNAs during early spermatogonial development in chicken and provided the basis for future research. |
| publisher |
Public Library of Science |
| publishDate |
2016 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4821617/ |
| _version_ |
1613562597422923776 |