Functionalization of an Antisense Small RNA

In order to explore the possibility of adding new functions to preexisting genes, we considered a framework of riboregulation. We created a new riboregulator consisting of the reverse complement of a known riboregulator. Using computational design, we engineered a cis-repressing 5′ untranslated regi...

Full description

Bibliographic Details
Main Authors:	Rodrigo, Guillermo, Prakash, Satya, Cordero, Teresa, Kushwaha, Manish, Jaramillo, Alfonso
Format:	Online
Language:	English
Published:	Elsevier 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4819895/

id	pubmed-4819895
recordtype	oai_dc
spelling	pubmed-48198952016-04-14 Functionalization of an Antisense Small RNA Rodrigo, Guillermo Prakash, Satya Cordero, Teresa Kushwaha, Manish Jaramillo, Alfonso Brevia In order to explore the possibility of adding new functions to preexisting genes, we considered a framework of riboregulation. We created a new riboregulator consisting of the reverse complement of a known riboregulator. Using computational design, we engineered a cis-repressing 5′ untranslated region that can be activated by this new riboregulator. As a result, both RNAs can orthogonally trans-activate translation of their cognate, independent targets. The two riboregulators can also repress each other by antisense interaction, although not symmetrically. Our work highlights that antisense small RNAs can work as regulatory agents beyond the antisense paradigm and that, hence, they could be interfaced with other circuits used in synthetic biology. Elsevier 2016-02-27 /pmc/articles/PMC4819895/ /pubmed/26756967 http://dx.doi.org/10.1016/j.jmb.2015.12.022 Text en © 2016 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Rodrigo, Guillermo Prakash, Satya Cordero, Teresa Kushwaha, Manish Jaramillo, Alfonso
spellingShingle	Rodrigo, Guillermo Prakash, Satya Cordero, Teresa Kushwaha, Manish Jaramillo, Alfonso Functionalization of an Antisense Small RNA
author_facet	Rodrigo, Guillermo Prakash, Satya Cordero, Teresa Kushwaha, Manish Jaramillo, Alfonso
author_sort	Rodrigo, Guillermo
title	Functionalization of an Antisense Small RNA
title_short	Functionalization of an Antisense Small RNA
title_full	Functionalization of an Antisense Small RNA
title_fullStr	Functionalization of an Antisense Small RNA
title_full_unstemmed	Functionalization of an Antisense Small RNA
title_sort	functionalization of an antisense small rna
description	In order to explore the possibility of adding new functions to preexisting genes, we considered a framework of riboregulation. We created a new riboregulator consisting of the reverse complement of a known riboregulator. Using computational design, we engineered a cis-repressing 5′ untranslated region that can be activated by this new riboregulator. As a result, both RNAs can orthogonally trans-activate translation of their cognate, independent targets. The two riboregulators can also repress each other by antisense interaction, although not symmetrically. Our work highlights that antisense small RNAs can work as regulatory agents beyond the antisense paradigm and that, hence, they could be interfaced with other circuits used in synthetic biology.
publisher	Elsevier
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4819895/
_version_	1613561874598592512

Functionalization of an Antisense Small RNA

Similar Items