Cytokine profile and lymphocyte subsets in type 2 diabetes

Type 2 diabetes mellitus (T2D) is a metabolic disease with inflammation as an important pathogenic background. However, the pattern of immune cell subsets and the cytokine profile associated with development of T2D are unclear. The objective of this study was to evaluate different components of the...

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Main Authors:	Francisco, C.O., Catai, A.M., Moura-Tonello, S.C.G., Arruda, L.C.M., Lopes, S.L.B., Benze, B.G., Del Vale, A.M., Malmegrim, K.C.R., Leal, A.M.O.
Format:	Online
Language:	English
Published:	Associação Brasileira de Divulgação Científica 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4819407/

id	pubmed-4819407
recordtype	oai_dc
spelling	pubmed-48194072016-04-25 Cytokine profile and lymphocyte subsets in type 2 diabetes Francisco, C.O. Catai, A.M. Moura-Tonello, S.C.G. Arruda, L.C.M. Lopes, S.L.B. Benze, B.G. Del Vale, A.M. Malmegrim, K.C.R. Leal, A.M.O. Clinical Investigation Type 2 diabetes mellitus (T2D) is a metabolic disease with inflammation as an important pathogenic background. However, the pattern of immune cell subsets and the cytokine profile associated with development of T2D are unclear. The objective of this study was to evaluate different components of the immune system in T2D patients' peripheral blood by quantifying the frequency of lymphocyte subsets and intracellular pro- and anti-inflammatory cytokine production by T cells. Clinical data and blood samples were collected from 22 men (51.6±6.3 years old) with T2D and 20 nonsmoking men (49.4±7.6 years old) who were matched for age and sex as control subjects. Glycated hemoglobin, high-sensitivity C-reactive protein concentrations, and the lipid profile were measured by a commercially available automated system. Frequencies of lymphocyte subsets in peripheral blood and intracellular production of interleukin (IL)-4, IL-10, IL-17, tumor necrosis factor-α, and interferon-γ cytokines by CD3+ T cells were assessed by flow cytometry. No differences were observed in the frequency of CD19+ B cells, CD3+CD8+ and CD3+CD4+ T cells, CD16+56+ NK cells, and CD4+CD25+Foxp3+ T regulatory cells in patients with T2D compared with controls. The numbers of IL-10- and IL-17-producing CD3+ T cells were significantly higher in patients with T2D than in controls (P<0.05). The frequency of interferon-γ-producing CD3+ T cells was positively correlated with body mass index (r=0.59; P=0.01). In conclusion, this study shows increased numbers of circulating IL-10- and IL-17-producing CD3+ T cells in patients with T2D, suggesting that these cytokines are involved in the immune pathology of this disease. Associação Brasileira de Divulgação Científica 2016-03-18 /pmc/articles/PMC4819407/ /pubmed/27007651 http://dx.doi.org/10.1590/1414-431X20155062 Text en http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Francisco, C.O. Catai, A.M. Moura-Tonello, S.C.G. Arruda, L.C.M. Lopes, S.L.B. Benze, B.G. Del Vale, A.M. Malmegrim, K.C.R. Leal, A.M.O.
spellingShingle	Francisco, C.O. Catai, A.M. Moura-Tonello, S.C.G. Arruda, L.C.M. Lopes, S.L.B. Benze, B.G. Del Vale, A.M. Malmegrim, K.C.R. Leal, A.M.O. Cytokine profile and lymphocyte subsets in type 2 diabetes
author_facet	Francisco, C.O. Catai, A.M. Moura-Tonello, S.C.G. Arruda, L.C.M. Lopes, S.L.B. Benze, B.G. Del Vale, A.M. Malmegrim, K.C.R. Leal, A.M.O.
author_sort	Francisco, C.O.
title	Cytokine profile and lymphocyte subsets in type 2 diabetes
title_short	Cytokine profile and lymphocyte subsets in type 2 diabetes
title_full	Cytokine profile and lymphocyte subsets in type 2 diabetes
title_fullStr	Cytokine profile and lymphocyte subsets in type 2 diabetes
title_full_unstemmed	Cytokine profile and lymphocyte subsets in type 2 diabetes
title_sort	cytokine profile and lymphocyte subsets in type 2 diabetes
description	Type 2 diabetes mellitus (T2D) is a metabolic disease with inflammation as an important pathogenic background. However, the pattern of immune cell subsets and the cytokine profile associated with development of T2D are unclear. The objective of this study was to evaluate different components of the immune system in T2D patients' peripheral blood by quantifying the frequency of lymphocyte subsets and intracellular pro- and anti-inflammatory cytokine production by T cells. Clinical data and blood samples were collected from 22 men (51.6±6.3 years old) with T2D and 20 nonsmoking men (49.4±7.6 years old) who were matched for age and sex as control subjects. Glycated hemoglobin, high-sensitivity C-reactive protein concentrations, and the lipid profile were measured by a commercially available automated system. Frequencies of lymphocyte subsets in peripheral blood and intracellular production of interleukin (IL)-4, IL-10, IL-17, tumor necrosis factor-α, and interferon-γ cytokines by CD3+ T cells were assessed by flow cytometry. No differences were observed in the frequency of CD19+ B cells, CD3+CD8+ and CD3+CD4+ T cells, CD16+56+ NK cells, and CD4+CD25+Foxp3+ T regulatory cells in patients with T2D compared with controls. The numbers of IL-10- and IL-17-producing CD3+ T cells were significantly higher in patients with T2D than in controls (P<0.05). The frequency of interferon-γ-producing CD3+ T cells was positively correlated with body mass index (r=0.59; P=0.01). In conclusion, this study shows increased numbers of circulating IL-10- and IL-17-producing CD3+ T cells in patients with T2D, suggesting that these cytokines are involved in the immune pathology of this disease.
publisher	Associação Brasileira de Divulgação Científica
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4819407/
_version_	1613561610472783872

Cytokine profile and lymphocyte subsets in type 2 diabetes

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